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  3. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...pair change in DNA gyrase, CRISPR phagemids were cytotoxic only for resistant bacteria. Bikard et al. used...

  6. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...containing the ccdB gene. Since the ccdB product is toxic to the cell, Gateway cloning efficiency can reach...

  9. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...to spontaneously develop resistance to selection toxins. Auxotrophic selection is useful for producing ...

  14. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...Yeast models of PD, like those that overexpress the toxic protein α-synuclein (the most common protein found...

  15. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...imidazolinone (DFHBI). DFHBI is membrane permeable and non toxic to cells. Because it rapidly photobleaches, DFHBI...

  19. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...methyltransferase for epigenetic silencing, which can be toxic, CHARM uses a cofactor fused to a histone tail to...

  20. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ... can be used, however, these are generally more toxic to cells than their plasmid counterparts. Another...
Showing: 101 - 120 of 135 results