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We narrowed to 389 results for: control

Showing: 101 - 120 of 389 results

  1. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...qPCR assays, samples, standard curves, and normal controls must be run in triplicate.  The nice thing about... of DNA in your sample relative to your normal control - you can set up your calculations so that your...so forth. This also serves as a light technical control - an RQ value of 1.5 means you have either made... does not require a standard curve or a normal control, it can take a few hours longer than qPCR to run...

  2. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ... Transcription Reporter: Placing GFP under the control of a promoter of interest can be used to effectively...Scientists can use light to detect, measure, and control molecular signals, cells, and groups of cells in...Plasmids of this type may have the GFP under the control of an additional promoter from that of the gene...fate studies. It can also be used, when put under control of promoters of interest, to visualize the developmental...

  3. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...tips on probe chemistries, primer optimization, controls, and analysis.  As a rule of thumb, you can assume...the fold change of each sample compared to your control. But as we said previously, just because it’s used...assumes the amplification kinetics of the target and control genes are fairly equal, an assumption that is not... Pfaffl et al No Standard Curve sample=control Gentle, et. al No Raw Data researcher defines...between 0 and 1. Flipping your experimental and control values during analysis can allow you to see this...differences between your experimental values and your controls. ANOVA, t-test, multiple regression analysis, ...-018-26707-8Suzuki T; Higgins PJ; Crawford DR. Control selection for RNA quantitation. Biotechniques. ...

  4. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    Published
    April 23, 2020, 1:15 p.m.
    ...One of the main tenets of our quality control (QC) mantra is to do our utmost to ensure that scientists...Gene Therapy. Existing methods for AAV quality control Historically, AAV vector QC has been sparse including...number of AAV preparations this level of quality control is sufficient but for viral vector core facilities...

  5. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...the origin of replication (ori) and all of its control elements. The ori is the place where DNA replication...depending on how they are regulated. Generally, control of replication is referred to as "relaxed" or "...Vectors Copy Number+ ori Incompatibility Group Control pUC ~500-700 pMB1 (derivative) A Relaxed pBR322...

  6. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...placing FP timer DsRed-E5 under the control of Neurog3 (a gene controlling pancreatic differentiation) in ...synthesized or long-lived transcription factors control gross changes in gene expression as new body sections...Pratik, et al. "A programmable synthetic lineage-control network that differentiates human IPSCs into glucose-sensitive...

  7. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...target genes. The sequence of the promoter region controls the binding of the RNA polymerase and transcription... all the time while others are more carefully controlled. Regulated promoters might act only in certain... light. In the cell, promoters themselves are controlled by still other regulatory factors: enhancers,...induced. Requires T7 RNA polymerase, which is also controlled by lac operator. Can be induced by IPTG. Commonly...

  8. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...streamline identification. While the Zhang lab controlled their multiplicity of infection so that a single...disease depends on both genetic and expression control systems to mimic illness on a molecular level. ...either with or without mCherry and GFP fluorescent controls. Alternatively, you can order individual plasmids...

  9. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...Promoters control the binding of RNA polymerase and transcription factors. Since the promoter region ...promoters can be regulated by positive or negative control. Positive inducible In the OFF state, the promoter...system, the activator rtTA (reverse tetracycline-controlled transactivator) is normally inactive and cannot...

  10. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    Published
    Dec. 18, 2018, 1:17 p.m.
    ...plasmids Anti-CRISPR proteins for optogenetic control of CRISPR-Cas9 Anti-CRISPR proteins that inhibit...has adapted anti-CRISPR proteins for optogenetic control of CRISPR-Cas9. By combining AcrIIA4, a Streptococcus...

  11. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...(4; brighter than control, 3; same as control (Cetn1/sgRNA1), 2; darker than control, 1; very dark). Brighter...mammalian cells was being explored and it allowed control over each individual component of the system. However...

  12. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...opposite chamber) [2]. Laser activation is usually controlled automatically using motion capture software such...targeted brain region after stimulation compared to controls without Chr2. c-Fos is a reliable and widely used...PMC4181379. 3. Kheirbek, Mazen A., et al. "Differential control of learning and anxiety along the dorsoventral ...silencer: development and application to optogenetic control of non-human primate cortex." Frontiers in systems...

  13. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...experiment. Research applications Gene expression control Researchers are always wanting to manipulate gene...depending on the virus used. All of these expression control mechanisms require a specific modification of the...that the copies of library cargo delivered are controllable. For many screening applications, it is essential...

  14. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type
    Blog Post
    Published
    Feb. 19, 2019, 1:36 p.m.
    ...” and making sure we have adequate process and controls in place to manage those scenarios. What is the...the effectiveness of the Plan's risk management, control, and governance processes. Prior to Tufts Health...Auditors and the Information Systems Audit and Control Association. Lastly, Carl also serves on the Board...

  15. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...of using loxP sites is the inability to tightly control which loxP sites recombine if more than two are...allowing for two separate recombination events to be controlled in parallel. Have you used the Cre-lox system...PubMed Central PMCID: PMC49604. 4. Independent control of immunoglobulin switch recombination at individual...

  16. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...reproduction. Both physiological processes are controlled by deliberate and compulsory actions instigated...complex can serve as a rate-limiting conduit to control the actions of the whole complex.  For example,...N. J.and O'Malley, B. W. (2002). Combinatorial control of gene expression by nuclear receptors and coregulators...

  17. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    Published
    Jan. 28, 2014, 3:10 p.m.
    ...competent cells, don’t forget to do a negative control (plate your cells without adding plasmid) to make...have contamination.  Also conduct a positive control (test a plasmid that transforms well in other competent...

  18. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ... under the control of the cI857 temperature-sensitive repressor. The temperature-controlled expression... at a dose and duration sufficient to kill all control cells and surviving “mutant” colonies were deep...GTPases involved in cell signaling pathways that control many cellular processes such as differentiation...gRNA backbone plasmids with Addgene. Additional control plasmids for the TKO library will be available ...luciferase activity in the Co-IP compared of a control Co-IP indicates stronger interaction. The system...Furthermore, dCAS9-VPR expression is under the control of the TRE promoter, which allows for doxycycline-inducible...result is genomic integration of dCas9-VPR and controlled expression of the dCas9-VPR transgene by doxycycline...

  19. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...to hook up your lasers and not have the proper controls or a proper behavioral readout that allows you...not be an appropriate test.  Of course, proper controls are required and include, for example, confirmation...Carolina) that offer his constructs. Importantly, control viruses (not expressing the opsin) are also available...into the correct direction and expressed (under control of a generic promoter). A DIO opsin allows cell...the fluorescent proteins are used as a critical control. Depending on the AAV serotype, promoter of choice...

  20. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...native regulatory elements. Controls for RaPID experiments RaPID negative controls include both scrambled ...enrichment) when compared to a scrambled RNA motif control). BioID2 had no detectable signal at the same time...
Showing: 101 - 120 of 389 results