We narrowed to 151 results for: cycs

1. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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   Blog Post

   Published

   May 11, 2021, 1:15 p.m.

   ...1988) Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of...

2. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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   Blog Post

   Published

   Jan. 31, 2019, 1:32 p.m.

   ...CAV 1. Usually, caveolin 1 promotes the cell life cycle progression and enhances tumor growth, so it would...

3. All in a Twist: dsRNA

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   Blog Post

   Published

   Feb. 2, 2023, 2:15 p.m.

   ...acting on specific substrates and in distinct cell cycles. When RNA:DNA hybrids levels are allowed to reach...

4. Choosing the B(right)est Fluorescent Protein: Photostability

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   Blog Post

   Published

   June 8, 2017, 2:30 p.m.

   ...light reduces the number of excitation/emission cycles per unit of time, decreasing the likelihood that...

5. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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   Blog Post

   Published

   April 13, 2017, 2:30 p.m.

   ...organelles to the vacuole/lysosome for degradation and recycling. The key to Rosella’s autophagy-sensing abilities...

6. CRISPRainbow and Genome Visualization

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   Blog Post

   Published

   Feb. 28, 2017, 3:30 p.m.

   ... to track telomere dynamics throughout the cell cycle. To optimize the signal to noise ratio of the system...

7. Popular Retroviral Vectors and Their Uses in Scientific Research

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   Blog Post

   Published

   July 24, 2018, 12:51 p.m.

   ...You can clone your gene of interest into this tetracycline inducible, 3rd generation lentiviral vector ...

8. Adenoviral Vector Production and Troubleshooting

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   Blog Post

   Published

   June 14, 2018, 11:44 a.m.

   ... are scraped, and lysed by multiple freeze-thaw cycles. The resultant lysate is your primary low titer...

9. Rabies and Neuronal Tracing

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   Blog Post

   Published

   May 29, 2018, 1:51 p.m.

   ...Once this first order infection occurs, subsequent cycles of viral replication and retrograde transmission...

10. Antibodies 101: Stripping and Reprobing Western Blots

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    Blog Post

    Published

    Aug. 1, 2024, 1:15 p.m.

    ...several times — some researchers report up to ten cycles! Just remember that a bit of sample is lost in ...

11. Viral Vectors 101: Virus Safety

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    Blog Post

    Published

    June 22, 2023, 1:15 p.m.

    ...meaning it can’t replicate and continue the infection cycle once inside the host. While the virus can still ...

12. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    Blog Post

    Published

    March 8, 2017, 3:15 p.m.

    ...nourseothricin (clonNat), spectinomycin, streptomycin, tetracycline, combinations of the above, or other. High...

13. 6 Tips for Grant Writing

    Type

    Blog Post

    Published

    Oct. 18, 2016, 2:30 p.m.

    ... to improve your application for the next grant cycle.  Many thanks to our Guest Blogger, Sean Mac Fhearraigh...

14. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type

    Blog Post

    Published

    Dec. 1, 2015, 3:30 p.m.

    ...Using the Tet-on system, his lab has created a tetracycline cas9 inducible knock-out system. His lab is ...

15. Hot Plasmids and Viral Preps - May 2021

    Type

    Blog Post

    Published

    May 18, 2021, 1:15 p.m.

    ...where gRNA expression is induced by anhydrous tetracycline. Three new anti-CRISPRs were identified that...

16. Academic vs. Industry Postdocs

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    Blog Post

    Published

    Nov. 13, 2018, 1:22 p.m.

    ...industry postdoc will free you of the never-ending cycle of grant-writing that is academic life. Of course...

17. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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    Blog Post

    Published

    July 13, 2017, 2:30 p.m.

    ...stop emerging at night. An incubator with a light cycle makes for very predictable work! When flies first...

18. Simplify Cloning with in vivo Assembly

    Type

    Blog Post

    Published

    Oct. 18, 2018, 12:37 p.m.

    ... different templates. All PCRs are run as an 18 cycle, 25 μl single-tube reaction. If you are synthesizing...

19. Course-Based Undergraduate Research Experiences (CUREs)

    Type

    Blog Post

    Published

    Feb. 9, 2023, 2:15 p.m.

    ...work on malate dehydrogenase (MDH), a citric acid cycle enzyme he studied during graduate school. As a stable...

20. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type

    Blog Post

    Published

    Sept. 8, 2016, 2:30 p.m.

    ... tag (Figure 1c). Click chemistry (e.g., transcyclooctene–tetrazine)– Nonnatural amino acids can be ...