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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 365 results for: erg

Showing: 101 - 120 of 365 results

  1. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...protect against parasites but also play a role in allergies. They are found bound to IgE receptors on the ...cells, two types of white blood cells involved in allergic reactions. There are no IgE subclasses and they...

  2. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ...this year with a fresh team of twenty excited undergrads. Team IISER-B’s idea for the iGEM 2019 is to ...Bhopal which received the Silver Medal in the Undergrad category at the iGEM Competition 2019. He has ...

  3. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ... products are then transformed into E. coli to undergo nick repair. This method can work well, but poor... starts out in familiar territory, but quickly diverges from previously discussed methods. As described...

  4. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ... Dept. of Biochemistry and Biophysics and the Lineberger Comprehensive Cancer Center at UNC Chapel Hill...j.molcel.2014.08.002 Ran FA, Hsu PD, Lin C-Y, Gootenberg JS, Konermann S, Trevino AE, Scott DA, Inoue ...

  5. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ... by ion channels and pumps in the membrane. An emerging interdisciplinary field of biology known as bioelectricity...Developmental bioelectricity is an extremely exciting emerging field, with many implications for regenerative...

  6. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...FUNCAT and PLA PLA was originally published by the Söderberg lab in Sweden as a method for the visualization...14166766. PubMed Central PMCID: PMC300121. 3. Söderberg, Ola, et al. "Direct observation of individual...

  9. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...batch to batch variability. Once an animal has undergone a terminal bleed to acquire all of its serum, ...https://doi.org/10.2144/btn-2018-0065 Fishman JB, Berg EA (2019) Antibody Purification and Storage. Cold...

  10. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    Published
    April 20, 2023, 4 p.m.
    ...learned along the way. Own your time When I was an undergrad, I was assigned a paper to give as a journal club... of a very confused group of visiting Chinese undergrads. You get nervous and freeze, or forget a point...

  11. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...and lox511 (GtATACAT). These variant lox sites undergo recombination with other sites of the same type...recombination. I. Recombination between loxP sites. Sternberg, N. and Hamilton, D. 1981. PubMed PMID: 6276558...

  12. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...the Auxin Inducible Degradation System (AID) The Dernberg lab in 2015 adapted the AID system discovered ...  Plasmids for cGAL and split cGAL systems The Sternberg lab in 2016 created cGAL, a GAL-4 based bipartite...

  14. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ...The stringent stripping solution uses heat and detergent to denature and remove antibodies but is also ...Tris HCl 62.5 mM Buffer SDS 2% (w/v) Detergent Β-mercaptoethanol (βME) 100–115 mM = 0.7%...

  15. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ... only hypothetical at this point. More time and energy must be devoted to studying Cas3 and its Cascade...S., Lin, C., Owen, A. L. G., Künne, T., . . . Sternberg, S. H. (2019). Harnessing type I CRISPR–Cas systems...

  17. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...tags into the genome of the pathogenic fungus Aspergillus fumigatus, which has been difficult to modify...mutagenesis by microhomology-mediated end joining in Aspergillus fumigatus.” Fungal Genet Biol. 86: 47-57. PMID...

  18. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...fluorescence [or Förster] resonance energy transfer). FRET occurs when energy transfer from one FP (e.g. CFP...

  19. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ... Pubmed PMID: 19363495. 4. Josse J, Kaiser AD, Kornberg A. Enzymatic synthesis of deoxyribonucleic acid... PMCID: PMC4409215. 7. Swartz MN, Trautner TA, Kornberg A. Enzymatic synthesis of deoxyribonucleic acid...
Showing: 101 - 120 of 365 results