...there are multiple amplicons, or primer dimers forming, the curve will show multiple peaks.    (a...Addgene frown upon statistical laxness in all its forms. Reporting your data  Once you’ve done the work ...share it so others can provide you with the highest form of flattery - imitation! Use the MIQE guidelines...most machines have a protocol that automatically performs the normalization step.  Machines Most general-use...machine. (Teo, et al, 2002; see paper for more information on optimizing for LightCycler machines.) Multiplexing...ensure you’re recording and reporting the right information so others can reproduce your efforts. And if ... J; Wittwer CT. The MIQE guidelines: minimum information for publication of quantitative real-time PCR...

...Morphology is a simple and fast to evaluate. iPSCs should form compact colonies that are comprised of cells with...functional tests. Cells can be differentiated in vitro to form embryoid bodies which are compact balls of loosely...offspring demonstrates the ability of the iPSCs to form functional germ cells. The highest stringency test...after differentiation has been linked to tumor formation in iPSC-derived and iPSC-transplanted mice. Cre-deletable...to purify the reprogramming proteins needed to perform these experiments. Assays for iPSCs Characterization...teratomas is the gold standard instead. Teratoma formation requires injecting iPSCs into immunodeficient ...

...for someone with HR expertise to join their newly formed compensation committee. The compensation committee...

... a variety of mammalian cell lines. Alternative forms of this vector contain GFP-tagged and un-tagged ...confusing to the uninitiated, contains much of the information needed to understand how to use it. TLDR: This... Zhang Lab's Nature Protocols Paper for more information). V2.0 This designation separates pSpCas9(BB)...

...under blue light CRY2-CIB1 heterodimerizes or forms clusters under blue light Check out our Optogenetics ... Rab11, and Rab35 to understand their roles in forming the cilia that help establish left-right organization...heterodimerizes under blue light BcLOV4 undergoes a conformational change and binds the plasma membrane under ...leading to behaviors like lamellipodia or filopodia formation, cell contraction, or other morphological changes...publications, they described tools to control formation of lamellipodia and contractile stress fibers,..., Rab11, and Rab35 coordinate lumen and cilia formation during zebrafish left-right organizer development...

...actionability on the Addgene blog and how SITN short-form blog posts help readers think through current events...a source of scientific information for the general public as well as a platform for scientists to be viewed... for listeners to understand the dynamics of information flow. Some of the topics they've recently covered...Mani is a graduate student at Harvard University, former Co-director of Science in the News, and member ... received her PhD from Harvard University, is a former Co-director of Science in the News, and is a member...

...exception. For the past 10+ years, Addgenies have formed teams, chosen a theme for the costume, and did ... the snail, the larvae reach the eyestalk and transform them into a pulsating display imitating a moving...lethal, associated with defective exoskeleton formations. These genes were appropriately named as spook...grasshoppers and crickets. When it’s ready to transform into an aquatic adult, it forces its insect host...

...et al., 2016). It’s generally accepted that some form of homologous recombination (HR) occurs via this...genomes encoding part A and part B of the transgene form a heterodimer, then splicing of the pre-mRNA of ...vector had an improvement in muscle physiological performance.  2. Trans-splicing Overview: For trans-plicing...et al., 2005). Trans-splicing does not always outperform single oversized AAVs (Hirsch et al, 2013). ...

... However, if repair material is provided (in the form of an oligonucleotide or plasmid), precise changes...still have much to learn from biology in all its forms! Many thanks to our guest bloggers, Wenning Qin...concept, straightforward to use, and robust in performance. In its natural setting, CRISPR-Cas9 is an acquired...

...production.   MANOVA The MANOVA is a multivariate form of ANOVA. Isn’t that what a two-way ANOVA test is...distribution. If your data is normally distributed, it will form (more or less) a straight line. If your data does... enough information. A post hoc test, done after your initial analysis can provide information on specifically...ANCOVA analysis. If you have performed a MANOVA/MANCOVA and want this information, multiple ANOVAs/ANCOVASs...play by the rules” and which we have certain information about. The assumptions and requirements that ...than one another, a two-tailed test should be performed. This test will assess if the mean of an experimental...tests, referred to as post-hoc analysis, must be performed. These will be discussed later. One and two-way...

...Overexpressed proteins also have the potential to form aggregates and may be toxic to the cell. Here comes...What can you do with SunTag? Tanenbaum et al. performed a variety of different experiments in their paper...

...engineered gene clusters that encode proteins that form bacterial gas vesicles. These ~200nm long vesicles...by an inexpensive and widely available imaging platform: ultrasound. Challenges of in vivo imaging with...ultrasound imaging. Ultrasound is an attractive imaging platform for in vivo imaging experiments because  ultrasound...Following in vitro characterization, Bourdeau et al performed a proof-of-concept experiment to demonstrate the...

... collection by filling out our Suggest a Plasmid Form. We look forward to helping microbiologists from...sharing and was also a great place to catch up with former colleagues! I ran into past ASM President Tim Donohue...

...technologies on our blog and encourage you to fill out this form if you’re ever interested in writing your own blog... upon you, our depositors, giving us as much information as possible during the deposit process. Special...

...While in Grad School How to Lead a Great Meeting Form Your Own Peer Mentoring Group...interdisciplinary seminars, such as those in bioinformatics or chemistry, even if your primary focus lies...

...to the biology and chemistry societies that will form the core of their scientific networks in their many...society also organises PhD information events and summer internship information sessions to help undergraduate...the answer is probably no, but these fun and informative events are one of the main ways budding young...intro sessions, and give out useful items and information to students. Freshers fairs are also where many...

...cationic liposome) or electrical (electric current forming membrane pores) transfection. These techniques ...sequence to be placed on the packaging plasmid forming a vector that could create replication competent... See the NIH’s biosafety guidelines for more information and always be sure to consult your institution...transduction for instance. I hope you will find this information useful for your experiments. Don’t hesitate to...check our lentiviral vector guide to get more information and to get access to additional protocols and...

...antibodies are produced from hybridomas. Hybridomas are formed by fusing B cells that have been extracted from... of the heavy and light chains fused together to form a single protein that can recognize the target protein...sequence of amino acids, or only in their 3D conformation. Types of antibodies and production In the immune...Addgene blog: Read about another reagent sharing platform for antibodies, the Developmental Studies Hybridoma...

...Internal Revenue Code 501(c)(3). That means we were formed to benefit the public, not private interests. On...improving access to useful research materials and information.” Addgene is here to help scientists accomplish...the world to gain access to new materials and information. Core values drive decisions at Addgene If you...customer service that is consistently timely, informative, friendly, and professional Addgene is a thought...

...can also find several other constructs in plasmid form. Fig. 1: Summary of the approach and outcomes...-2P. Using a custom, high-throughput screening platform to apply saturating mutagenesis on 21 positions...experiments with mouse cortical neurons, far out-performing previous attempts to engineer potassium-selective...engineering to shift the spectra and improve the performance of HcKCRs. Find HcKCR plasmids here!   Govorunova...