We narrowed to 336 results for: nam
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TypeBlog PostPublishedJuly 18, 2019, 12:55 p.m....articles published there as well. Worm Development Dynamics Database (WDDD) The WDDD aims to acquire quantitative... Kyoda, Koji, et al. "WDDD: worm developmental dynamics database." Nucleic acids research 41.D1 (2012)...
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CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog PostPublishedJuly 23, 2020, 1:20 p.m....anti-CRISPR nomenclature Acr family proteins are named for the type of CRISPR-Cas system they block and...light, or small molecules could achieve rapid, dynamic, and spatial control of CRISPR-Cas activity. For... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog PostPublishedMay 7, 2020, 1:15 p.m....components: a deaminase (TadA, originally from E.coli, named TadA7.10 in ABE7.10) a catalytically impaired Cas... ABE7.10 accordingly which resulted in variants named “ABE8.x”: “8” stands for the eighth round of ABE... -
Hot Plasmids: Winter 2025
TypeBlog PostPublishedMarch 11, 2025, 1:15 p.m....Mike Lacy In order to study the conformational dynamics of the motor protein kinesin-1, the Twelvetrees...single-molecule FRET to study the intramolecular dynamics of the proteins in solution and when associated... -
Antibodies 101: Flow Compensation
TypeBlog PostPublishedMay 16, 2024, 1:15 p.m....compensate between them. Another rule of thumb is if the names of the fluorescent markers are similar, you will...Chart. Pro tip! Some antibodies go by different names depending on your flow cytometer’s setup. For instance... -
PhD Applications After COVID
TypeBlog PostPublishedJan. 5, 2023, 2:15 p.m....program. Most applications will recommend that you name two professors in your application that you intend...reference your questions about the PI’s research or the name of the award you won for your conference presentation... -
Tips to Make the Most of a Scientific Conference
TypeBlog PostPublishedMay 23, 2019, 12:26 p.m....want to include your university and your advisor’s name for some context and maybe common ground. The people...also helpful to write your Twitter handle on your nametag for the tweep up (and for the entire conference... -
Polymerase Chain Reaction Overview and Applications
TypeBlog PostPublishedAug. 10, 2021, 1:15 p.m.... analyzed. End point PCR End point PCR, as the name implies, analyzes the end product of PCR temperature...Quantification.” Multiplex PCR Multiplex PCR, as the name implies, is a method in which multiple targets can... -
Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR
TypeBlog PostPublishedJuly 2, 2020, 12:40 p.m....differentiation. CAPTURE 2.0 captures chromatin's dynamic role in gene regulation The high resolution and... capture of spatial configuration and temporal dynamics of locus-specific 3D chromatin by biotinylated... -
Chromoproteins: Colorful Proteins For Molecular Biology Experiments
TypeBlog PostPublishedFeb. 4, 2020, 2:15 p.m....bioluminescent phytoplankton, and colorful corals, to name a few. What’s behind these sights are an abundance...important to note that despite their distinction by name, chromoproteins are actually a subset of the fluorescent... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog PostPublishedJune 22, 2017, 2:30 p.m....to your organelle of interest. Click on the gene names in the figure below to find plasmids containing ...Ruusuvuori, Pekka, et al. "Quantitative analysis of dynamic association in live biological fluorescent samples... -
Fluorescent Proteins 101: Fluorescent Protein Timers
TypeBlog PostPublishedMay 4, 2017, 2:30 p.m....protein timers FP timers have been used to: Monitor dynamics of vesicle fusion and release from the plasma ...overtime (Subach et al.) Monitor protein expression dynamics in a synthetic circuit (Saxena et al.) In a general... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog PostPublishedApril 19, 2016, 2:30 p.m....applications of FPs is to visualize the localization, dynamics, and behavior of a POI. As an investigator, you...Membrane proteins fused to FPs that dimerize can form dynamic contacts that bring apposing membranes together... -
AAVs in Retinal Gene Therapy
TypeBlog PostPublishedJuly 31, 2018, 12:55 p.m....recessive, severe, childhood-onset form of blindness name Leber’s Congenital Amaurosis (LCA). Shortly after...vectors to support trials for prevalent diseases - to name a few. CRISPR-based tools are also particularly... -
An Introduction to Adenovirus
TypeBlog PostPublishedOct. 3, 2023, 1:15 p.m....Kafri et al., 1998). In fact, adenovirus gets its name because it was first isolated from human adenoid...targeted cells is less likely for adenoviral vectors – namely due to the removal of certain adenoviral genes ... -
Tips for arabidopsis transformation
TypeBlog PostPublishedOct. 25, 2018, 1:23 p.m....from studying transcriptional and translational dynamics of genes and proteins in living plants, to complementing... plant selection markers (basta, hygromycin, kananamycin, tunicamycin) (Nakagawa et al., 2007). Alternatively... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog PostPublishedOct. 6, 2015, 2:30 p.m....majority of plasmids contain either an ampicillin or kanamycin resistance gene. Every step along the way involves...the container holding the tubes and use this ID to name the corresponding Excel sheet.3. Be specific - ... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog PostPublishedMay 15, 2014, 3:33 p.m....green portion of the visible spectrum (hence the name), which is due to a chromophore formed from a maturation...expressed in living cells, enabling the study of dynamic, physiological processes. Re-engineering GFP to... -
Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research
TypeBlog PostPublishedJuly 29, 2025, 1:15 p.m....Institute. When it comes to genome editing, CRISPR is a name that resonates with nearly every biologist, academic...resistance gene as the plant-selectable marker and a kanamycin resistance gene for bacterial selection. The process... -
CRISPR 101: Any Base Transversion Editors
TypeBlog PostPublishedApril 1, 2025, 1:15 p.m....track of which acronym is which. Acronym Full name Notes Base transition Base change between purines...same protein (or its homologs) has more than one name. Indel Insertion/deletion A type of unwanted DNA...