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We narrowed to 274 results for: sars

Showing: 101 - 120 of 274 results

  1. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...prevent cutting at mismatched guide sites, but not necessarily binding to these sites. Thus, these enzyme swaps...perform whole genome sequencing. While this may be unnecessary for many applications, it is the only comprehensive...

  2. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...Systems Together You may be wondering, “why is it necessary to have all 3 of the different binary systems ...similarly?” The simple answer is that sometimes it is necessary or desirable to control or look at multiple genes...

  3. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...knockdown or overexpression experiment, generate the necessary constructs, and complete screening in just 3 days...This toolkit reduces the expense and workload necessary to produce vectors for genome editing in worms...the initiation of the T. gondii infection, and necessary for the asexual cycle of malaria parasites. Find...

  4. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...packaging plasmid containing all the structural genes necessary for the production of a viral particle (Gag, Pol...encoding the insert of interest as well as sequences necessary to encapsulate that insert into a viral particle...

  5. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...missing? For example, are additional controls necessary? As you build your story, are additional data ...important to have a plan to get to mechanism. Do the necessary tools exist to tackle the problem and if so, do...

  6. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...represented below are those of the writers and do not necessarily represent the views of Addgene. Boston, MA, ...breast cancer and infertility or to argue against unnecessary clinic regulations, evidence-based research is...

  7. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...sections and 3D cultures These samples do not necessarily require optical sectioning, but open the door...by pixel are unable to provide the frame-rates necessary for imaging tissues of this size. For example,...

  8. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...the antibody you expect it to produce is not necessarily what you are getting. Learn more about monoclonal... Because the entire antibody molecule is not necessary for antigen binding, the variable regions alone...

  9. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...work? What are the various tools and components necessary to run it? And, most importantly, what are the...lab focuses primarily on behavior, you don’t necessarily need to use electrophysiology to prove that your...

  10. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...includes a lot of acronyms, so we’ve included a glossary at the end if you ever need to double-check your... closer than ever. References and Resources Glossary We introduced a lot of terminology in this post...

  11. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...conditions were generated in your lab are necessary for use of a plasmid you are depositing can ...the submitted material under the terms deemed necessary by my institution or other third party rights ...

  14. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...under the stringently low expression conditions necessary to avoid biological perturbation in a variety ... additional iterations of Level 1 assembly if necessary. Combining multiple Level 2 vectors in the final...

  15. Twenty Years of Sharing Science!

    Type
    Blog Post
    Published
    Jan. 8, 2024, 3:25 p.m.
    ...Today marks Addgene's 20th anniversary! We've done so much in the past two (2!) decades that it's hard...

  16. AAV Packaged on Request is Here!

    Type
    Blog Post
    Published
    Jan. 10, 2025, 1:01 p.m.
    ...After an order is placed, we’ll facilitate the necessary MTA, then start production and quality control...
Showing: 101 - 120 of 274 results