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We narrowed to 243 results for: virus

Showing: 101 - 120 of 243 results

  1. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    .... The TOPO technique specifically uses Vaccinia virus-isolated topoisomerase I as this enzyme recognizes.... Shuman S. "Recombination mediated by vaccinia virus DNA topoisomerase I in Escherichia coli is sequence...

  2. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...full-fledged pandemic. Containing the spread of this virus has become an utmost priority for countries around...method has been optimized to detect the SARS-CoV-2 virus directly using saliva without having to perform ... not give a positive result for highly similar viruses. Unlike Cas12a, Cas13a does not require strict ... methods.   Rapid detection of 2019 novel coronavirus SARS-CoV-2 using a CRISPR based DETECTR assay...in the saliva (Lucia et al., 2020). Detecting coronavirus using CRISPR-based SHERLOCK (Specific ​H​igh ​...ultrasensitive and visual detection of novel coronavirus SARS-CoV-2 Sensitivity: 4.6-11 copies/microliter...2020). An ultrasensitive, rapid, and portable coronavirus SARS-CoV-2 sequence detection method based on...

  3. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    Published
    June 1, 2021, 1:15 p.m.
    ...mammal called alpaca could neutralize the SARS-CoV-2 virus. But while the early results were promising, the...projects to a halt. None of us works directly with viruses or transmissible diseases, so we were left with...origins trace back to March 2020, when the first coronavirus lockdown forced us to put our research projects...

  4. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ... transfection efficiency when delivering these viruses via retro-orbital vs. tail vein systemic injections...Additional Resources on the Addgene Blog Using virus in your research - a primer for beginners Adenoviral...user Resources on Addgene.org Find Adenovirus Plasmids Adenovirus Guide Page Subscribe to Viral Vectors...

  6. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...familiar, ready-to-use adeno associated virus (AAV) and lentivirus are now available from Addgene. While...repository, you can instead have Addgene make the virus for you. Neuroscientists are particularly interested...

  7. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ...now important for accelerating research into this virus. One such example is cell lines. The American Type...Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-...Infected with Severe Acute Respiratory Syndrome Coronavirus. JVI 81:813–821 . https://doi.org/10.1128/jvi...

  8. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...Santana et al., 2020) and plants viruses (barley yellow striate mosaic virus (Gao et al., 2019) or sonchus... the ubiquitously expressing cauliflower mosaic virus 35S promoter (CaMV 35S) or ubiquitin promoters (...sonchus yellow net rhabdovirus (Ma et al., 2020) have already been shown to be efficient ways to deliver CRISPR-Cas9...Z, Wang Y, Wang X (2019) Rescue of a plant cytorhabdovirus as versatile expression platforms for planthopper...

  9. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...have a resource like Addgene to all get the same virus.” — Grad student visiting Addgene’s booth at the...Addgene pivoted to support researchers combatting the virus. Our COVID-19 collection, comprised of 1302 plasmids...

  10. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...for improvement of optogenetic adeno-associated virus (AAV) vectors. Listed below are a few additional...Sabatini BL. 2012. Novel recombinant adeno-associated viruses for Cre activated and inactivated transgene expression...

  11. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...neural pathways was 8.5 x 10e10 GC/mL. The lot of viruses used (100798-AAV, lot #v15287, titer 1.7x10e13 ... (2018) An Alternate Route for Adeno-associated Virus (AAV) Entry Independent of AAV Receptor. J Virol...Monosynaptic Tracing Success Depends Critically on Helper Virus Concentrations. Front Synaptic Neurosci 12. https...

  14. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    Published
    Jan. 10, 2018, 4:13 p.m.
    ...proteins are a key component of lentiviruses. These proteins coat the virus and help determine the types ... of 2017 is the lentivirus envelope plasmid pMD2.G from Didier Trono’s lab! Lentivirus envelope vector... producing our ready-to-use lentivirus preps. You can find our lentivirus production protocol on our website...titering your lentivirus in another blog post. If you need a quick primer on how lentiviruses work, check...widely used and requested because it enables lentiviruses to infect many different types of cells. This...check out our lentivirus guide page. For more protocols and information, be sure to check out the Trono Lab...Additional Resources Quick Guide to All Things Lentivirus 5 Tips for Troubleshooting Viral Transductions...

  16. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ... enable the virus to enter host cells. To do this, they injected the spike protein from two different ...spike proteins in cell culture, and inhibit these viruses.  But llama immunization has its drawbacks: it...bind to coronavirus spike proteins (Wrapp et al., 2020), which sit on the surface of coronaviruses and enable...different coronaviruses into a llama over several days and isolated special camelid antibodies that bind to the...Structural Basis for Potent Neutralization of Betacoronaviruses by Single-Domain Camelid Antibodies. Cell ... carriers collection Find other COVID-19 and Coronavirus Plasmids & Resources ...

  17. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ... that allow the bacteria to target and destroy viruses that infect them. Commonly referred to as a bacterial...

  18. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...improve the catalytic efficiency of tobacco etch virus protease (TEV), a highly sequence-specific protease... from SARS-CoV-2 sequences and live influenza A virus. It was demonstrated to work in human lung epithelial...

  19. Hot Plasmids: Summer 2025

    Type
    Blog Post
    Published
    Aug. 28, 2025, 1:15 p.m.
    ...labs engineered the tobacco rattle virus (TRV), a bipartite RNA virus, to deliver a TnpB (named ISYmu1)...

  20. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...almost three decades, recombinant Adeno-associated virus (AAV) has been widely used to deliver genetic tools..., myelocytomatosis viral oncogene (myc), simian virus 5-derived epitope (V5), the synthetic peptide FLAG...
Showing: 101 - 120 of 243 results