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We narrowed to 842 results for: Eras

Showing: 591 - 600 of 842 results
  1. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    Published
    July 21, 2020, 1:15 p.m.
    ...reading, you can calculate the DNA concentration. Generally, A260 of 1.0 is equivalent to 50 ug/ml pure dsDNA...samples and are often used to quantify DNA for next generation sequencing. Unlike absorbance-based methods, .... This method is often used preceding next-generation sequencing or microarray studies and is not used...
  2. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ... need a second GFP antibody?  Similarities:  Generated against full length GFP and converted to recombinant... also withstands denaturing conditions, high temperatures, and other chaotropic conditions better than...fluorescence in high molarity GdnHCl, at high temperatures, and in the presence of oxidizing agents. Adapted...
  3. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    Published
    Nov. 7, 2019, 2:18 p.m.
    ...minor variation in experimental conditions will derail a research program (think a subtle difference in...to help your fellow researchers advance science overall. Communication is the issue Much of the problem...President. Her current responsibilities encompass overall leadership of the Cell Press editorial department...
  4. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ..., why it affects our emotions, and even what therapeutic properties it has. As with other works of Dr....of characters from five different families over several millennia as their paths cross with each other ...Nicholson Baker’s The Anthologist. The book ended up literally changing my life. Though I have an undergraduate...
  5. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...landmark Cell paper showing that it was possible to generate induced pluripotent stem cells (iPSCs) capable...has spent years studying how that one protein interacts with that one promoter can tell you, it can be...clinical trial patients with advanced macular degeneration; Laurenz Studer discussed his work with Parkinson's...
  6. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ... with a simple question: how do Cas9 and sgRNA interact with and dissociate from a DNA target? The surprising...et al., 2016. We next wondered if we could leverage this model (built from in vitro studies) to boost.... Figure 2: Single-stranded donor DNA interaction with the Cas9-DNA duplex. Image from Richardson...
  7. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    Published
    July 28, 2017, 1:30 p.m.
    ...and UAS plasmids at Addgene Choosing a vector to generate a transgenic fly There are a few important points... when choosing the vector you’d like to use to generate your new fly line: 1. Where would you like the...  et al. Cytokine/Jak/Stat signaling mediates regeneration and homeostasis in the Drosophila midgut. Cell...
  8. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ... the “Analyze Sequence” button to pull up an interactive plasmid map powered by SnapGene and gain access...the static maps discussed above, but also have interactive functionalities. You can now hover over any feature...maps, the linear sequences hosted here are now interactive and have similar hover over features. As with...
  9. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ...protein is based on the properties found in the literature or tabulated data that summarizes properties ...). However, the theoretical brightness ignores several important experimental conditions that are related...detector sensitivity) and the biological system (temperature, prokaryote versus eukaryote, background fluorescence...
  10. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ...out of these interactions, ask yourself how every piece of feedback you receive generalizes.  If someone...skillset rather than a permanent fix. Treat every interaction in your work as an opportunity to practice communicating...
Showing: 591 - 600 of 842 results