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We narrowed to 837 results for: Eras

Showing: 591 - 600 of 837 results

  1. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    Published
    July 21, 2020, 1:15 p.m.
    ...reading, you can calculate the DNA concentration. Generally, A260 of 1.0 is equivalent to 50 ug/ml pure dsDNA...samples and are often used to quantify DNA for next generation sequencing. Unlike absorbance-based methods, .... This method is often used preceding next-generation sequencing or microarray studies and is not used...

  2. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ... with a simple question: how do Cas9 and sgRNA interact with and dissociate from a DNA target? The surprising...et al., 2016. We next wondered if we could leverage this model (built from in vitro studies) to boost.... Figure 2: Single-stranded donor DNA interaction with the Cas9-DNA duplex. Image from Richardson...

  3. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    Published
    Aug. 19, 2025, 1:15 p.m.
    ... the trace; asterisk indicates a single AP. B) Averaged fluorescence responses to increasing numbers of...International license. Using OCaMP in your lab Several OCaMP constructs are now available through Addgene.../article/28248224/","type":"EXTERNAL"},"user_generated_content":false}{% endraw %}{% end_module_attribute...

  4. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    Published
    June 25, 2019, 12:54 p.m.
    ...Transfection is the process by which foreign DNA is deliberately introduced into a eukaryotic cell through non-viral...diethylaminoehtyl (DEAE)-dextra neutralize or even impart an overall positive charge on DNA molecules so that it can.... "Chitin induces natural competence in Vibrio cholerae." Science 310.5755 (2005): 1824-1827. PubMed PMID...

  5. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    Published
    July 28, 2017, 1:30 p.m.
    ...and UAS plasmids at Addgene Choosing a vector to generate a transgenic fly There are a few important points... when choosing the vector you’d like to use to generate your new fly line: 1. Where would you like the...  et al. Cytokine/Jak/Stat signaling mediates regeneration and homeostasis in the Drosophila midgut. Cell...

  6. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ... the “Analyze Sequence” button to pull up an interactive plasmid map powered by SnapGene and gain access...the static maps discussed above, but also have interactive functionalities. You can now hover over any feature...maps, the linear sequences hosted here are now interactive and have similar hover over features. As with...

  7. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ...protein is based on the properties found in the literature or tabulated data that summarizes properties ...). However, the theoretical brightness ignores several important experimental conditions that are related...detector sensitivity) and the biological system (temperature, prokaryote versus eukaryote, background fluorescence...

  8. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ...out of these interactions, ask yourself how every piece of feedback you receive generalizes.  If someone...skillset rather than a permanent fix. Treat every interaction in your work as an opportunity to practice communicating...

  9. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ... how stretches of DNA on different chromosomes interact with each other. Chromatin regulation is complex...in the right direction! CAPTURE-ing Chromatin Interactions: Using CRISPR-dCas9 to Study Gene Regulation... An Improved Method for Studying Protein-DNA Interactions CRISPRainbow and Genome Visualization CRISPR...

  10. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...closely associated and function as one unit: Gβγ. In general, GPCRs show a propensity to associate with certain...receptor, which can then activate expression of a luciferase reporter in the nucleus. The Roth lab showed ...Chemogenetics uses genetically engineered receptors to interact with small molecules to elicit a response in a...
Showing: 591 - 600 of 837 results