...talk at the Cell Symposia: Gene- and Cell-based Therapies: CRISPR, Stem Cells, and Beyond meeting earlier...III. Gene III is under the control of a T7 RNA polymerase and cannot be expressed until the base editor...to an uninfected culture which results in less overall dilution and less stringent phage selection. ...

...Plasmids 101 will provide you with an overview of general molecular biology knowledge and techniques. If ...with only white colonies is very suspicious! Refrigerate your plates: Placing plates at 4C for few hours...care in making your plates: X-gal is light and temperature sensitive and needs to be added to media after...

...CRISPR-Cas9 and a custom gRNA is all you need to generate a frameshift mutation that will permanently ablate...package cDNAs for genomic delivery and have many therapeutic advantages, such as tissue-specific targeting... to temporarily increase expression. There are several flavors of activators which can be used with this...

...from peer-reviewed publishing in a journal.  Accelerating Scientific Communication Preprints have been...Fig. 1: Stand aside, CERN - it's a new age of accelerating science! Photo courtesy of under a CC license...attendees to read and comment online! Want the general public (say, policymakers or community organizations...

...exposed to 500 nm violet light, the Dronpa dimers interact and lock MEK1 in an “off” setting by blocking ...possible to create other photoswitchable kinases? The general design of psMEK1 also worked for creating three...all-optical psMEK1 + ERK KTR-mRuby2 system has several advantages over existing methods. First, it avoids...

...the photostability measurements reported in the literature are performed in different ways. We describe ...intensity remains. The first issue is that, in several cases, the fluorescence intensity decay of a fluorophore... be identified by a head-to-head comparison of several fluorescent proteins of the same spectral class...

...implementing consensus recommendations, even when a federal mandate encourages them to do so. Immediately after...There are institutions in Boston - Massachusetts General Hospital, Mass Eye and Ear Infirmary and Beth Israel...salaries when all others in Boston (some of them literally across the street from these institutions) are...

... of this is gospel, but we think it's worth consideration. Everything will go wrong Whenever an order ... lab, a similar situation could arise if a lab veteran who worked with a particular sample suddenly left...easily as needed. In this case, the physical considerations of the laboratory make object composition a...

... randomly. Therefore, inserting loxP sites at deliberate locations in a DNA sequence allows for very specific...reciprocal crossover event. This event can have three general outcomes based on the location and orientation ...separate DNA molecules, a translocation event is generated at the loxP sites. How can I use Cre-lox? The ...

...and think of ways to relate the proposal and deliverables to the aims requested in the call. Step 2: ...technologies. Therefore, take time to review the literature and offer educated insight into what the desirable...each aim. A funded proposal typically lists the deliverables, explains 1-2 potential pitfalls of the intended...