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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 836 results for: Eras

Showing: 601 - 610 of 836 results

  1. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...Plasmids 101 will provide you with an overview of general molecular biology knowledge and techniques. If ...with only white colonies is very suspicious! Refrigerate your plates: Placing plates at 4C for few hours...care in making your plates: X-gal is light and temperature sensitive and needs to be added to media after...

  2. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    ...exposed to 500 nm violet light, the Dronpa dimers interact and lock MEK1 in an “off” setting by blocking ...possible to create other photoswitchable kinases? The general design of psMEK1 also worked for creating three...all-optical psMEK1 + ERK KTR-mRuby2 system has several advantages over existing methods. First, it avoids...

  3. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...the photostability measurements reported in the literature are performed in different ways. We describe ...intensity remains. The first issue is that, in several cases, the fluorescence intensity decay of a fluorophore... be identified by a head-to-head comparison of several fluorescent proteins of the same spectral class...

  4. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ...implementing consensus recommendations, even when a federal mandate encourages them to do so. Immediately after...There are institutions in Boston - Massachusetts General Hospital, Mass Eye and Ear Infirmary and Beth Israel...salaries when all others in Boston (some of them literally across the street from these institutions) are...

  5. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    Published
    Feb. 16, 2023, 7:05 p.m.
    ...CRISPR-Cas9 and a custom gRNA is all you need to generate a frameshift mutation that will permanently ablate...package cDNAs for genomic delivery and have many therapeutic advantages, such as tissue-specific targeting... to temporarily increase expression. There are several flavors of activators which can be used with this...

  6. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...from peer-reviewed publishing in a journal.  Accelerating scientific communication Preprints have been...Figure 1: Stand aside, CERN — it's a new age of accelerating science! Photo courtesy of Henry Mühlpfordt,...attendees to read and comment online! Want the general public (say, policymakers or community organizations...

  7. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ... of this is gospel, but we think it's worth consideration. Everything will go wrong Whenever an order ... lab, a similar situation could arise if a lab veteran who worked with a particular sample suddenly left...easily as needed. In this case, the physical considerations of the laboratory make object composition a...

  8. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ... randomly. Therefore, inserting loxP sites at deliberate locations in a DNA sequence allows for very specific...reciprocal crossover event. This event can have three general outcomes based on the location and orientation ...separate DNA molecules, a translocation event is generated at the loxP sites. How can I use Cre-lox? The ...

  9. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...and think of ways to relate the proposal and deliverables to the aims requested in the call. Step 2: ...technologies. Therefore, take time to review the literature and offer educated insight into what the desirable...each aim. A funded proposal typically lists the deliverables, explains 1-2 potential pitfalls of the intended...

  10. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ... and biological. Even the most high-fidelity polymerases can make mistakes and lead to the accumulation...populations. All of these topics require additional considerations to resolve. Sequencing and assembly issues ...acquired from the MiSeq for assembly or with enough coverage over plasmid regions to confirm their presence...
Showing: 601 - 610 of 836 results