... and biological. Even the most high-fidelity polymerases can make mistakes and lead to the accumulation...populations. All of these topics require additional considerations to resolve. Sequencing and assembly issues ...acquired from the MiSeq for assembly or with enough coverage over plasmid regions to confirm their presence...

...select the components they want for each module, generate the vector file, and quickly clone their vector... Step 4: Naming your assembled plasmid and generating the vector file on the Fragmid website. ...4, is to give the assembled plasmid a name and generate the vector file. Ta-da! Most (180/181) Fragmid...

... the right choice. I spoke with our Financial Operations Specialist, Lucy Hammer, about why she did not...question came from Lucy Hammer, our Financial Operations Specialist. She said, “I’ve found the most purpose...the office. Main takeaways from Meet an Addgenie Overall, the Meet an Addgenie project was one of the biggest...

...format that was stable for up to a year at room temperature (Pardee et al, 2014). As a demonstration of the...the project by initially designing and ordering several dozen versions of the toehold switches and amplification...Addgene As we report, we have embedded this next generation of toehold switch-based RNA sensors within an...

...in AT-rich genomes. Additionally, whereas Cas9 generates blunt ends 3 nt upstream of the PAM site, Cpf1...with Kleinstiver et al. reporting that Cpf1 can tolerate mismatches at gRNA positions 1, 8, 9, and 19-23...sticky end” transgene insertion mediated by Cpf1-generated overhangs. Exploring the efficiency of these two...

...and mutant loxP sites has allowed scientists to leverage this system in new, creative ways. Today’s post...potential to express the transduced reporter gene. Generating a FLEx switch to control expression of an optogenetic...Double-floxed inverse Orientation), FLEx systems are generally considered "Cre-On" meaning that your gene of ...

... modulators, and chimeric effectors. Nucleases generate a classic double-stranded break, while modulators...with researchers all over the world. That’s an average of 27,272 plasmids distributed per year, or approximately...it clear that it’s also a story of community, cooperation, and sharing. Sharing this story is a truly special...

...specific recombination outcome using PCR and then generate a monoclonal cell line with the floxed exon. You...B, A Limaye, & AB Kulkarni. (2009) "Overview: Generation of Gene Knockout Mice". Curr Protoc Cell Biol...Basics of Homology Directed Repair Read about Generating Knockouts with CRISPR through Non-Homologous ...

...drawn to the idea of working as a team to develop therapies that could directly impact patients. And, to be...beginning of March, and the jobs are mixed into the general careers section of their web site. Each position... and is applying this towards researching neurodegenerative diseases.   Additional resources on the Addgene...

...needed for EM is not something you find in the average cell and molecular biology lab, and EM is limited... is able to expand evenly in all directions to several times its original size. Depending on the protocol...depend upon the specific gel recipe used, ambient temperatures, incubation times, and the physical properties...