We narrowed to 836 results for: Eras
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TypeBlog PostPublishedJune 20, 2023, 1:15 p.m....needed for EM is not something you find in the average cell and molecular biology lab, and EM is limited... is able to expand evenly in all directions to several times its original size. Depending on the protocol...depend upon the specific gel recipe used, ambient temperatures, incubation times, and the physical properties...
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What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry
TypeBlog PostPublishedJuly 1, 2025, 1:15 p.m....Figure 1B-D). These other molecules can alter the overall 260 nm reading, distorting the final concentration...with dilute samples: Take multiple readings to average out noisy measurements. Be cautious when interpreting...these long enough? There are, of course, more considerations and other approaches for measuring DNA quantity... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog PostPublishedNov. 29, 2022, 2:15 p.m....deprioritize genes that could be good targets for therapeutic development, particularly in cancers that are... ancestry which will reduce the likelihood of generating genetic dependency data with an ancestry-based...researching ancestry groups. And, of course, it’s imperative for scientists to be aware of and actively use... -
Cpf1: A New Tool for CRISPR Genome Editing
TypeBlog PostPublishedOct. 14, 2015, 2:30 p.m.... Prevotella and Francisella), was annotated in several genomes and is classified as a type V CRISPR system...Again, they were in for a surprise! Whereas Cas9 generates blunt ends 3 nt upstream of the PAM site, Cpf1...with Kleinstiver et al. reporting that Cpf1 can tolerate mismatches at gRNA positions 1, 8, 9, and 19-23... -
A Career in Grants: How To Become a Grant Professional
TypeBlog PostPublishedAug. 20, 2015, 2:30 p.m....the grantor’s requirements as well as state and federal regulations. A grant professional’s job duties ...Dr. Cook served as a principal investigator for several research grants. One of the benefits of being a...management, and program evaluation are highly transferable. Use your experience and your passion(s) to ... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog PostPublishedOct. 25, 2016, 2:30 p.m....are below the new exemption level (the current average postdoc salary is estimated at around $45,000, ...tests must be met. In the Department of Labor’s general guide to the effect on higher education, you can...Perez, Fair Pay for Postdocs: Why We Support New Federal Overtime Rules, made clear that institutional arguments... -
How to Be an Excellent Trainee
TypeBlog PostPublishedSept. 26, 2023, 1:15 p.m....scientific mentor is typically a professor who operates a research group. Within that research group, ...everything! The bare minimum should be taking notes on general protocol steps and other tips and tricks your mentor...my dissections go smoother next time?” or more generally “How am I doing? Can you tell me what’s going ... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog PostPublishedOct. 19, 2017, 1:54 p.m....sites differ at the nucleotide level but share an overall structure of 13 base pair palindromic repeats separated...efficient The first version of FLP discovered has a temperature optimum of 30 °C and is therefore inefficient...led to the identification of FLPe which has a temperature optimum of 37 °C (Buchholz, Angrand, and Stewart... -
An Integrin Antibody Toolkit from IPI
TypeBlog PostPublishedMarch 28, 2024, 1:15 p.m.... with low affinity for ligands. When integrins interact with extracellular ligands, their ectodomains ...sites and do not interfere with integrin function. Overall, the synthetic rAbs demonstrate remarkable specificity...cells. What’s next IPI is working with Addgene to accelerate biomedical research by distributing antibodies... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog PostPublishedSept. 1, 2015, 2:30 p.m....still go horribly awry when several factors aren’t taken into consideration. 1. Always focus on the science...equipment that you use in your current lab first. Generally, major equipment can be defined as equipment with...