Addgene

We narrowed to 705 results for: Tat

Showing: 621 - 630 of 705 results

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Optimizing Donor DNA for Enhanced CRISPR Genome Editing

Type

Blog Post

Published

March 24, 2016, 2:30 p.m.

...if breaking the gene is worse than leaving the mutation alone). CRISPR delivery by nucleofection The in...
Addgene Welcomes John Doench to the Advisory Board

Type

Blog Post

Published

Aug. 13, 2015, 2:30 p.m.

...during a lecture given by Phil Sharp. After a few rotations, Doench decided to complete his PhD work in Sharp...
Plasmids 101: Mammalian Vectors

Type

Blog Post

Published

March 25, 2014, 3:15 p.m.

...Resistance Cell Types* Mode of Action** Working Concentation*** Blasticidin bsd HeLa, NIH3T3, CHO, COS...
GCE4All: Making Genetic Code Expansion Accessible

Type

Blog Post

Published

June 15, 2023, 1:15 p.m.

...GCE4All, an NIH-funded center located at Oregon State University focused on turning GCE tools developed...
Viral Vectors 101: Viral Applications

Type

Blog Post

Published

April 13, 2023, 1:15 p.m.

... have been engineered to introduce frameshift mutations in genes of interest, turning expression off permanently...
Viral Vectors 101: Gamma-Retroviral Packaging Systems

Type

Blog Post

Published

Aug. 6, 2024, 1:15 p.m.

...by long terminal repeat (LTR) sequences, which facilitate integration of the transfer plasmid sequences...
Developing a cold tolerant E. coli using specialized chaperones

Type

Blog Post

Published

Jan. 28, 2020, 2:35 p.m.

... means recombinant proteins can aggregate or precipitate out as insoluble granules. We overcame this problem...
Behind the scenes: Addgene’s new search engine and more

Type

Blog Post

Published

Dec. 12, 2019, 2:22 p.m.

...engine unique. Most importantly, with this new implementation, we laid the foundations for many improvements...
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

Type

Blog Post

Published

Nov. 5, 2019, 1:28 p.m.

...approaches, knowledge of both the advantages and limitations of each Cas enzyme can help determine which enzyme...
Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

Type

Blog Post

Published

June 25, 2019, 12:54 p.m.

...discovered in 1946 by Joshua Lederberg and Edward Tatum when they observed genetic recombination between...

Showing: 621 - 630 of 705 results

Results per page:

Optimizing Donor DNA for Enhanced CRISPR Genome Editing

Addgene Welcomes John Doench to the Advisory Board

Plasmids 101: Mammalian Vectors

GCE4All: Making Genetic Code Expansion Accessible

Viral Vectors 101: Viral Applications

Viral Vectors 101: Gamma-Retroviral Packaging Systems

Developing a cold tolerant E. coli using specialized chaperones

Behind the scenes: Addgene’s new search engine and more

Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

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