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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 836 results for: Eras

Showing: 631 - 640 of 836 results

  1. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...with a 2:1 BSc in Molecular Biology (roughly a B average in the American system), in 2009 (at the beginning...structured and controlled. I worked from Standard Operating Procedures (SOP’s) and every item I tested was...sustainable. After working at this company for several years, I was ready for a change.  Working abroad...

  2. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...resonance energy transfer (FRET) is a biophysical interaction between two fluorophores. When the first fluorophore...& Zhang, 2006), quantifying antibody/antigen interactions (Saraheimo et al., 2013), and visualizing structural...different biomolecules you’d like to test for interaction. In fact, targeting a FRET fluorophore to a biomolecule...

  3. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...multiplex gRNAs, some being: Using dual nickases to generate a knockout or edit. This can help reduce off-target...gRNAs, with four being the ideal number. First you generate four unique kanamycin-resistant plasmids, each...each unused promoter. This step is necessary to generate all of the overhangs needed for the final ligation...

  5. DIY DNA Ladders from Penn State University

    Type
    Blog Post
    Published
    July 14, 2017, 2:30 p.m.
    ...restriction digested with PstI or EcoRV, these plasmids generate 100 bp or 1 kb DNA ladders, respectively. Unlike.... L., & Tan, S. (2017). The pPSU Plasmids for Generating DNA Molecular Weight Markers. Scientific Reports...

  6. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    ...hydroelectric in nature. The operation of -80C and -20C freezer temperatures, biosafety cabinet ventilation...

  7. Addgene Protocols Go Viral: AAV Purification and Titration

    Type
    Blog Post
    Published
    June 13, 2019, 12:19 p.m.
    ...viral lots. Aligning with Addgene’s mission to “accelerate research and discovery by improving access to...calculations on how to prepare a plasmid stock used to generate a standard curve, visit the AAV Titration by qPCR...

  8. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ...outlets meaning you’ll have the opportunity to interact with and discuss your work with many different...applying CRISPR for mouse genome editing. Post: Generating Mouse Models Using CRISPR/Cas9   Erik L. Snapp...

  10. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    Published
    April 13, 2018, 12:51 p.m.
    ...Genetics Institute in Cambridge, MA and Sequana Therapeutics in San Diego, CA. She earned a B.S in Biology...Addgene as the Chief Financial Officer and Chief Operating Officer. Prior to Addgene, Kenneth was an investment...
Showing: 631 - 640 of 836 results