We narrowed to 836 results for: Eras

1. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

   Type

   Blog Post

   Published

   Aug. 13, 2014, 3:49 p.m.

   ...lab at the University of Oxford is dedicated to generating new tools to manipulate biology based on molecular...together, to produce irreversible protein-peptide interactions. In a study published in Proceedings of the ...

2. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

   Type

   Blog Post

   Published

   Feb. 20, 2014, 4:57 p.m.

   ...Genome wide mouse CRISPR library The Yusa lab has leveraged the CRISPR system to target all 19,150 mouse protein...Koike-Yusa H, Li Y, Tan E-P, Del Castillo Velasco-Herrera M, Yusa K. Nature Biotechnology. 2013 Dec 23. (...

3. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

   Type

   Blog Post

   Published

   June 11, 2019, 1:16 p.m.

   ...science in my blog posts. This post highlights several of these free tools which will hopefully also help...body, cellular biology, medical specialities, and general items. Individual images as well as collections...

4. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

   Type

   Blog Post

   Published

   March 15, 2018, 12:59 p.m.

   ...vary. In the PAM-out configuration, D10A tends to generate small deletions, while H840A is biased towards... likely due to the distinct overhang patterns generated by the nickases; D10A creates 5’ overhangs and...

5. Oh, The Places You Can Go: Careers in Science Communication - Product Development

   Type

   Blog Post

   Published

   Oct. 18, 2017, 1 p.m.

   ..., writing won out. “I considered teaching at a liberal arts school but I’ve always enjoyed writing.” In...measure, here’s a few other posts about developing transferable skills while in grad school Resources on Addgene.org...

6. SunTag and Fluorescent Imaging

   Type

   Blog Post

   Published

   March 28, 2017, 2:30 p.m.

   ...mitochondria without organelle toxicity. First generation (v1) SunTag is expressed at very low levels due...Visit Our Fluorescent Protein Guide Page Find Luciferase Plasmids ...

7. Addgene at the ASM Microbe 2016 Conference

   Type

   Blog Post

   Published

   July 7, 2016, 2:30 p.m.

   ... a learn a lot from these ASM pros and hope to leverage their expertise as we advance our mission of sharing...well-optimized CRISPR screening technology. Addgene got several shout-outs for making popular screening libraries...

8. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

   Type

   Blog Post

   Published

   Sept. 12, 2023, 1:15 p.m.

   ...tools, but here are a few stand-alone sites for general primer design, as well as primer design for different...cloning applications. Primer3 and Primer3Plus: For general primer design Design primers for Goldengate cloning...

9. Build Your CRISPR Vocabulary

   Type

   Blog Post

   Published

   July 2, 2024, 1:15 p.m.

   ...sequence as the scaffold. gRNA: "Guide RNA" is a general term referring to the RNAs used by Cas proteins...order to cut DNA, whereas some mismatches can be tolerated outside this region. Conclusion Ready for some...

10. Bricking Science: Portraying Scientific Reality Through LEGO

    Type

    Blog Post

    Published

    Nov. 29, 2016, 3:30 p.m.

    ... Oliveira. Bricking Science is an idea built, literally, 'brick-by-brick' to introduce people all around... video where he/she/it/something is captured on camera: Unfortunately, unless they’ve worked in a lab...