We narrowed to 932 results for: Gene

...began working remotely recently? Most of us at Addgene began working from home last week to flatten the...new. We usually have yoga class twice a week at Addgene HQ, but now we’re sharing links to online yoga ...before bed will help you prepare for sleep. At Addgene, our mindfulness group is sticking with their Headspace...virtually connected with the people in your life. At Addgene, we’ve started virtual activities like team lunches...being safe. @GHP_HarvardChan — Shekhar Saxena (@ssaxenageneva) March 16, 2020 Give back to your community...societies hosting virtual networking events. Download Addgene’s Science Career guide to learn more about career...framework to design your future experiments.  Use Addgene’s AAV Data Hub. You can search the AAV Data Hub ...

... pAAV.hSynap.iGABASnFR (112159-AAV1) Optogenetics Optogenetics continues to be a useful method for manipulating...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...serotype testing Calcium sensors and GABA sensors Optogenetics Recombinases And more! Control AAV For many...Recombinase AAV Targeting and manipulating specific, genetically-defined neuronal populations with intersectional...pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE (104496-AAVrg) Optogenetics pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (55645-...55645-AAV5)  Additional resources on the Addgene blog Read all of our viral vectors blog posts Find tips...

...more. Being that this is the Addgene Blog, I’ll, of course, mention Addgene as a source of sequenced and...event, I gave my own talk on reproducibility at Addgene and here I summarize what I learned both in preparation...31 - 49:45 - Reagent Sharing - Susanna Bachle, Addgene the nonprofit plasmid repository 49:45 0 1:18:45...new ways of understanding how the body works, generating new therapeutics, and sometimes formulating new...viral vectors. When working with a plasmid from Addgene, you can be confident that you’re using the actual...hosted by individuals and by organizations like Addgene (a good example is our series of blog posts on ...Initial positive results and the excitement they generate can, however, bias us. We’ve all heard stories...

...https://www.addgene.org/browse/article/28248224/","href_with_scheme":"https://www.addgene.org/browse/article...University of Calgary. Over the past few decades, genetically encoded calcium indicators (GECIs) have become...Several OCaMP constructs are now available through Addgene. Whether you're working with cell cultures, slice..." %}{% raw %}"Find OCaMP plasmids available at Addgene!"{% endraw %}{% end_module_attribute %}{% module_attribute.../article/28248224/","type":"EXTERNAL"},"user_generated_content":false}{% endraw %}{% end_module_attribute...2025.07.28.667269. Additional Resources on the Addgene Blog Viral Vectors 101: Calcium Sensors New Neuroscience...Fluorescent Protein Additional Resources on addgene.org Fluorescent Proteins: Biosensor Plasmids In-...

...and Jennifer Doudna Labs used targeted mutagenesis to generate hyper accurate Cas9 (HypaCas9). This nuclease...frequently used Cas9, spCas9 from Streptococcus pyogenes, this sequence is 3’-NGG. But Cas9s found in other...exhibit enhanced site accessibility In order to generate a genomic alteration at a specific location, it... sites. Using a combined random and directed mutagenesis approach, the Anna Cereseto Lab created pX-evoCas9...the Jungjoon Lee Lab used directed evolution to generate Sniper-Cas9 resulting in a Cas9 with improved ...PMCID: PMC6255809.  Additional resources on the Addgene blog Read our CRISPR 101 blog posts Find more ... Download the CRISPR 101 eBook Resources on Addgene.org Visit our CRISPR plasmids and resources page...

... on the Addgene blog An Overview of AAV Encoded Calcium Biosensors Illuminating Epigenetics with a ... systems that measure biological phenomena by generating an output which is directly proportionate to ...antibody, enzyme, DNA, etc. The receptors job is to generate a quantitative signal upon recognizing the analyte... (fluorescence, pH, etc.). The transducer will generate a numerical value which represents the amount ...: Almost all of the biosensors discussed are genetically encoded, and luckily, we have pre-designed plasmids...disciplines! Most of the sensors designed for calcium generate a fluorescent output, which will turn on, change.... DOI: 10.3389/fncel.2019.00474 Resources on Addgene.org Fluorescent Proteins: Biosensors Viral Service...

...developed a dual virus system using both optogenetics and chemogenetics to study the activity of parvalbumin...in the lab. He has recently developed a third-generation intensiometric glutamate sensing fluorescent ...catalyzes the desulfuration of 3-mercaptopyruvate and generates an enzyme-bound hydropersulfide that is then transferred...withdrawal before they occur.  First, she used a genetic method to tag neurons activated by opioid withdrawal...light activates the neurons (via oChIEF) and a chemogenetic agonist inhibits the neuron (via hM4D). She ...postdoc at Harvard University where she engineers genetically encoded voltage indicators. Tian developed a ...optimize a red‑shifted archaerhodopsin‑derived genetically encoded voltage indicator which had improved ...

...are tumor microenvironments made of?  A tumor’s genesis is divided into 3 phases: initiation, progression...that can be transferred to other cells, inducing epigenetic changes in targeted cells. In this case, exosomes...formation by inhibiting cell growth, invasion, and angiogenesis (Allinen et al., 2004). In fact, Sanford H. ...influence tumor microenvironments: immunology and angiogenesis (Witz et al., 2009). Then, scientists began ...Fernando Schmitt. "Microenvironment in breast tumorigenesis: Friend or foe?." Histology and histopathology...PubMed PMID: 20036813. Additional resources on the Addgene blog Read our cancer related blog posts Learn ...about the tumor microenvironment Resources on Addgene.org Visit our cancer research plasmids and resources...

...and learn more about Addgene’s viral service. Additional resources on the Addgene blog Download our Viral...service, and now we’re “going viral” again! #AddgeneGoesViral on Twitter Starting today, our Twitter feed... in the graphic below. Follow the hashtag #AddgeneGoesViral to find information on the basics of viral...applications, and some new resources available from Addgene. There’s a lot to talk about! And by the way, there...reagents and data, we’re also publishing research. Addgene’s research team also investigates new strategies... posts Learn more about AAVs Resources on the Addgene website Visit the AAV Data Hub Learn more about...

...mouse inner ear. Fourth-generation cytosine base editors The fourth-generation base editors, BE4, reduce...activity.  Find CRISPR base editing plasmids at Addgene Improving cytosine base editing scope and efficiency...18 bases upstream of the PAM.  David Liu’s lab generated BE3 variants with other deaminases: AID, CDA1,...these three improvements represent the fourth generation of base editors. Compared to BE3, BE4 offers ... phage-assisted evolution selection systems to generate ABE8e(TadA-8e V106W), which edits ~590-fold faster...., 2020). This is important because ABEs have generally been slow, meaning that the Cas9 domain often ... N. M. (2023). Improved cytosine base editors generated from TadA variants. Nature Biotechnology, 41(5...