CRISPR 101: Cas9 Nickase Design and Homology Directed Repair
Type
Blog Post
Published
March 15, 2018, 12:59 p.m.
...vary. In the PAM-out configuration, D10A tends to generate small deletions, while H840A is biased towards... likely due to the distinct overhang patterns generated by the nickases; D10A creates 5’ overhangs and...