We narrowed to 842 results for: Eras

...Second-generation vectors are likely sufficient for most experiments; however, the 3rd-generation packaging... 2nd vs. 3rd Generation Lentiviral Plasmids Q3: How can you tell 2nd- and 3rd-generation transfer vectors...considered safer. Second-generation transfer plasmids MUST be packaged with a 2nd-generation system because the... Third-generation plasmids can be packaged with either system. Please note that the generation used to...limiting the interaction between viral LTR enhancers and the transgene; such interactions can alter expression...production (3 or 4). Third-generation vectors contain gag, pol, and rev. Second-generation contains those three...and the plasmid coding for an envelope. Three generations of lentiviral packaging systems have been developed...

...protein-protein interactions, RNA-protein interactions, and even more recently cell-cell interactions. If you’... binary protein-protein interaction as well as identify additional interacting proteins in one simple ...RNA-protein interaction detection So far, we’ve covered ways to study protein-protein interactions, but scientists...RNA-protein interactions that can regulate multiple biological functions. RapID (RNA-protein interaction detection... mammalian cells (Milo, 2013). The number of interactions between these proteins is hard to imagine yet...yet alone study. To elucidate these interactions scientists have long used antibody based affinity purification...proximity-based labeling to better capture these interactions in a vast array of biological contexts.  Proximity...

...sequences. Degenerate primers Using degenerate primers is similar to error-prone PCR. Degenerate primers ...benefits antibody-based therapeutics. Whether antibodies are used directly as therapeutic agents or serve as...the same general cyclical process (Figure 2). The differences typically come in when generating the library... 2: General process of antibody directed evolution. Created with BioRender.com.   Generating mutant...high-fidelity polymerase to ensure accuracy. In this case, you would want a low-fidelity polymerase, like Taq...antibody engineering, to improve reagents and therapeutics. What is antibody engineering (and why do we...achieve this goal, including error-prone PCR, degenerate primers, gene shuffling, and structure-guided...

...Apart from fibroblasts, human keratinocytes from hair pluck, peripheral blood cells, and renal epithelial...Efficient generation of transgene-free human induced pluripotent stem cells (iPSCs) by temperature-sensitive...Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes. Nat Biotechnol,...Mukherjee. The ultimate goal in the field of regenerative medicine is to replace lost or damaged cells...cell is converted to a different cell type for regeneration and repair and situations where one process ...reverts back to a pluripotent state, which then proliferates and is redifferentiated to a different cell ...stage (iPSC formation) [2, 3]. The iPSCs then proliferate and redifferentiate to another cell type of choice...

...molecular interactions in the cell, including protein-protein, protein-DNA and protein-RNA interactions. The...case a reporter gene (e.g. luciferase or GFP), regardless of whether it interacts with a transcriptional ... the split-luciferase system, intein-mediated splicing reconstitutes functional luciferase only when fusions... fusions of the two halves of the luciferase protein interact and enable intein splicing (5). Screening...Umezawa, Y.l. Split luciferase as an optical probe for detecting protein-protein interactions in mammalian cells...create a new system for screening protein-protein interactions. In later years, improvements to yeast transformation...the classic two hybrid system (also called the interaction trap), genes encoding proteins of interest(POI...

...her shirt. She rolled several long takes, and we cut it up in post.  Test: Overall, it worked. We pulled...someone to be comfortable on camera while thinking about things like lighting, camera angle, and shot composition...and wearing their mask. But while the camera is here, the operator is in a different room.   Design... best possible videos for free. We’re a small operation, so sometimes it can be a big challenge. But ask...had a couple options. The first was to put me on camera, filming myself. While easiest from a production...protocol and can guide viewers through. I know cameras, but we needed someone else. Design: Jennifer thankfully... the office, we use a Black Magic Pocket Cinema Camera 4K. It’s wonderful and simple to use, but when ...

...treatments, such as surgery, radiation, and chemotherapy, immunotherapy is coming to the fore as a cutting edge...performs a heated chemotherapy wash throughout the abdominal cavity. This targeted chemotherapy approach has...susceptible to up and coming immunotherapies. The standard definition of immunotherapy is the use of drugs (or...research opportunities are being provided to immunotherapy than ever before. Although different types of...great promise in up and coming treatments like immunotherapy. Mesothelioma Mesothelioma is a terrible disease...first mesothelioma symptoms in their body. The average latency period (time from first asbestos exposure... certain other naturally occurring asbestiform minerals such as erionite, are the only scientifically ...

...best). The moderator takes control It is the moderator's job to prevent anyone from derailing the show....Choose a moderator with a backbone. It is nice to have a subject area expert, but the moderator is not ...LinkedIn profiles of all panelists to the moderator. The moderator and organizers should develop a list of... the interaction is not interesting to the audience. It is a bit of an art, but active moderation gets...effortless to the audience, but the organizers and moderator should have a well-planned approach to making ... of 4-6 pre-event interview questions. The moderator (possibly with an organizer) should set up a 30 minute...to schedule. In the pre-event interviews, the moderator should be taking good notes and discovering interesting...

...you need a high-fidelity polymerase. There are many high fidelty polymerases on the market; you need one...a DNA polymerase that produces blunt-ended PCR products (e.g. Phusion, Pfu, and Vent polymerases). Note...Note that certain polymerases such as Taq generate A-overhangs (a feature used in TA-cloning). Such non-complementary...site in the coding region (preferably near the 5’-end) and that also generates 4 bp overhangs. You can then... then use the Klenow fragment of DNA polymerase I to generate blunt ends from these overhangs and ligate... analyzing the role of renaturation temperature in the polymerase chain reaction." Analytical Biochemistry...this age of CRISPR; site-directed mutagenesis generally applies to plasmids, but may also facilitate genome...

...far, with safety increasing in each generation. The 1st generation of lentiviral vectors consisted of ...third-generation lentiviral vector system offers the best safety profile in terms of RCL generation because...2nd and the 3rd generation of vectors have been shown. However, with the 3rd generation you get lower viral...-create a functional virus. Indeed, by testing several vectors and packaging cell line combinations, Miller...were produced in high-titer retroviral stocks generated by the transfer of a retrovirus into the packaging...vectors Although retroviruses are still in use, considerable efforts have been devoted to develop efficient...infect non-dividing cells. Thus three different generations of lentiviral vectors have been established so...