... expression. However, one caveat of FP-protein fusions (FP-chimeras) is that they undergo normal protein...their continuous excitation. By generating PA-FPs fused to intracellular proteins, scientists can track ...as organelle trafficking and dynamics (fission, fusion, etc). For example, by engineering PA-GFP tagged...Youle Lab was able to visualize and quantify the fusion dynamics of mitochondria in healthy and apoptotic... individual organelles shows that mitochondrial fusion is blocked during the Bax activation phase of apoptosis...

...generating GFP fusion proteins. From considering protein size and shape, linker sequences, and fusion location...they’re free. 3. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection by Joachim...control and flexibility to CRISPR editing. Using a fusion between Cas9 and reverse transcriptase and a prime...

...desired (Figure 1). The probe perfuses liquid, which causes the passive diffusion of DA across the semipermeable...extracellular fluid into the perfused liquid. The probe then collects the perfusion liquid for quantitation...

... of a quickly-degraded destabilized GFP variant fused to a 4X CDRE repeat. In addition to showing that...2Fmic2020.04.713 Truncated TALE-fluorescent protein fusions as a DNA staining dye Article contributed by Jennifer...Kyubong Jo's lab generated fluorescent protein fusions with tTALE to “stain” DNA. TALE proteins can bind...structural deformation or photo-induced damage. These fusions can also function in high salt conditions - up ...

...that bind to cobalamin-fused fluorescent dyes; binding of the cobalamin fusion to the riboswitch results...containing the growth promoting domain. Each half was fused with one of the LOVTRAP domains. When both halves...quantification of base editors that utilize dCas9 fused to cytidine deaminases like APOBEC. To achieve this...

...protein. The prime editor consists of a Cas9 nickase fused to a reverse transcriptase. The Cas9 nickase is ...strand. The first iteration of prime editor simply fused the wild-type Moloney Murine Leukemia Virus (M-MLV...thermostability. What came out on top? The Cas9 nickase fused to a pentamutant of M-MLV RT. They called this system...3′ tail of pegRNAs (Yan et al., 2024). The team fused La to the C-terminal end of PEmax to produce PE7...pegRNA stability ~ ~ ~ ~ ~ ~ ✖ Fuse RNA-binding protein La to the prime editor Improve...

...demonstrate that they can successfully create GFP fusion proteins with a variety of genes across the genome...dehydrogenation). MitoTimers are composed of a Timer protein fused to the mitochondrial targeting sequence of cytochrome...perform multiple site mutagenesis and create complex fusion or recombination vectors. The efficient and flexible... is a monomeric, 27 kD genetic tag which can be fused to proteins of interest for EM imaging, or targeted...colleagues, FokI-dCas9 expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain...single reaction, while retaining only a defined 4bp fusion site in between. The MoClo system is comprised...

...domain.  How do these components interact? When fused together to form an OptoNB, the AsLOV2 domain acts...Since Ras is a membrane localized GTPase, the team fused a mCherry OptoNB to the catalytic domain of the ...that activates Ras. They expressed this OptoNB-SOS fusion protein in cells that expressed membrane-localized...activity Read about RANbodies, reporter nanobody fusions Learn more about using the secondary nanobody toolbox...

... delivery into mammalian cells, especially when fused to effector proteins for gene activation or gene...the new system was able to activate expression by fusing dCas12f to VP64. Read the paper in Nature Biotechnology...combines an endogenous Gag homolog, cargo RNA, and fusogen in a modular system for delivering gene editing...active Cas9 enzyme. PE-Cas9 comprises an active Cas9 fused to the reverse transcriptase (RT), which induces...

...its target.  They name their most potent Acr-LOV fusion protein inhibitor CASANOVA for “CRISPR-Cas9 activity...works with the S. pyogenes Cas9, dCas9-effector fusions, and xCas9, and requires no additional chemicals...plasmid tools which includes plasmids encoding a fusion between cPYP and tgRFP (PLL7.0 tgRFP cPYP), and...or dark- state of the photoswitchable proteins) fused to mVenus linked to a mitochondrial localization...