We narrowed to 740 results for: NSI

...A major limitation of APEX was its low sensitivity. Sensitivity could be increased with higher expression... avidin, proximity labelling can capture weak/transient protein interactions as well as be used for both...promiscuous biotin ligase (Kim et al 2016). BioID2 consists of a humanized biotin ligase from A. aeolicus,...cell.   Find BioID2 plasmids here APEX2: A more sensitive ascorbate peroxidase for proximity labeling A ...aggregation. The Ting lab hypothesized that the low sensitivity was due to suboptimal folding or stability. Thus...identified a mutant with significantly increased sensitivity that did not affect the function or efficiency... assemble when in close proximity. Split-BioID consists of a split BirA* molecule that can reassemble ...

... same considerations apply as for standard IF. However, there are a few specific considerations to keep...standard immunofluorescence, but with some extra considerations (Im et al., 2019; McLaughlin, 2019).    ...a good place to get started.  One important consideration when deciding whether you can do simultaneous...suitable antibody against your target protein, consider fusing a protein tag to the target and using an...epitope-based strategy such as Anti-6xHis. You could also consider strategies using other types of affinity reagents...your experiment, you might need to quantify the intensity of the different signals in a certain region or...optimizing your blocking and washing conditions or consider using a different antibody.  Prepare a positive...

... are considering a career at a small, teaching college you might want to consider less expensive, portable...low salary I might add). You have a right and responsibility to choose a lab where you can thrive and do...Former lab members can give you a fuller picture. Consider talking to people in the lab across the hall or... hope to grow.  Find mentors who take their responsibility to train you seriously. Why work for someone...whether or not a lab head is a good role model, consider questions like: How long do lab members stay ...allocation of meaningless tasks, removal of responsibility, repeated reminders of blunders, setting target...equipment quotes? Understand administrative responsibilities? Is the work (topic and/or technical aspects...

...postsynaptic receptors and transporters. The intensity-based Glutamate-Sensing Fluorescent Reporter (iGluSnFR) designed...this post: Glutamate indicators with increased sensitivity and tailored deactivation rates Addgene's own...interneurons Glutamate indicators with increased sensitivity and tailored deactivation rates By Mike LacyGlutamate...significantly improved brightness, kinetics, and sensitivity. iGluSnFR4f is well-suited for imaging rapid ... (2025). Glutamate indicators with increased sensitivity and tailored deactivation rates. bioRxiv, 2025.03.20.643984...vector that contains everything needed to replicate inside a host cell but with space to insert your genetic..., GPC3, and GPC4). All IPI antibodies undergo extensive characterization at IPI as well as community-led...

...variation between iPSC clones. Retroviruses are not considered safe for clinical applications.     Lentiviral...Like retroviruses, lentiviral delivery is not considered safe for clinical applications. Excisable Cre-lox...same as using a standard lentiviral vector, but transient expression of Cre recombinase allows for deletion... site of excision, this approach is still not considered safe for therapeutic applications. piggyBac (...insertional mutagenesis, adenoviral vectors are considered a safer way to express reprogramming factors ...many passages. Ban et al developed a temperature sensitive Sendai virus which is removed by culturing cells... regulate Sendai virus transgene expression. Transient episomal delivery Non-integrating methods have...

...capacity of stem cells. Yet, very few researchers consider the sex of their cells when doing experiments—...between male and female cells. The result of not considering the sex of the stem cells was that their male... in Norway, the team realized they should also consider the sex of the stem cells. In this case, they ... many researchers I’ve talked to who’ve never considered the sex of their cells. For one thing, cells ... there are numerous methodological articles to consider—we have loaded those we are aware of in our checklist...clue or potential place to start research is by considering sex disparities in disease severity. Such disparities...checklist. A common worry for researchers wanting to consider sex is cost. Especially in animal research, they...

...Cleavage Assay Not Provided 5% Inexpensive, simple Low-throughput, low sensitivity Sanger Sequencing Provided...inappropriate for heterogenous populations, low sensitivity Targeted Amplicon Sequencing Provided 0.01%...0.01% (variable) Sequence-level data, extremely sensitive Do not sequence all DSBs, may miss unpredicted ...information from targeted amplicon sequencing is highly sensitive with detection levels as low as .01% (Hendel et... with in silico prediction to create a more comprehensive list of off-target editing events. Two of the...laboratories. Nevertheless, unbiased detection isn’t as sensitive as targeted amplicon sequencing. For example, ...throughout the genome. However, their decreased sensitivity means that the best option moving forward may...

...available with improved kinetic and sensitivity parameters. Sensitivity and Localization The calcium concentrations...are used to monitor calcium concentrations and transients in living cells and organisms. Unlike chemical...kinetics: GCaMPf (fast) GCaMPm (medium), GCaMPs (sensitive but slower). GCaMPf may be suitable for very rapid...You want to use a calcium sensor that is most sensitive to your expected range. For example, calcium in... changes with Ca while another wavelength is insensitive. Additional red sensors based on mRuby (jRCaMP1...unnecessary calcium buffering and to increase sensitivity), and is a great FRET sensor option if you have...integration within the target cells, ensuring more consistent and reproducible results in calcium assays. There...

...and potential delivery methods.   General considerations for planning your CRISPR experiment As with...experiment, there are many factors that need to be considered during the planning process. For CRISPR experiments... method: Do you need stable integration or is transient expression sufficient? Which cell types will you...are compatible with all your components. Next, consider the best form for those CRISPR components based...selection marker to generate stable cell lines. Transient or stable expression of Cas9 and/or gRNA in a ...transfer vector and used to generate AAV particles. Transient or stable expression of SaCas9 and/or gRNA. Infects...cells using microinjection or electroporation. Transient expression of CRISPR components, expression decreases...

...showed greater photostability and also matured considerably faster, with expression levels comparable to...PILATeS, Pulling-Induced Luciferase Association Tension Sensor (Zhong et al., 2024). In this new variation...and only exposed when the module unfolds under tension. When this happens, BiT and the LgBiT luciferase... Figure 5: Left: Mechanism of the PILATeS tension sensor. The LgBiT (cyan) fragment of luciferase...protein (purple) only when Titin I10 unfolds under tension. Upon binding, LgBiT and BiT Tag catalyze a luminescent...PILATeS-functionalized coverslip creates mechanical tension, and the luminescence colocalizes with the focal...lower background signal and can be used in light-sensitive systems. Most excitingly, assembly of the split...