We narrowed to 318 results for: Mut

...engineering goals expand beyond generating frameshift mutations or single base pair edits, these enzymes are worth...

...fragments. However, rigidity can be introduced through mutation, allowing diabodies to be used for assembling ...

... gRNA is all you need to generate a frameshift mutation that will permanently ablate protein expression...

...inflammatory response. However, if dsRNA sensors are mutated or mechanisms which suppress endogenous dsRNA formation...

... the protein. To monitor autophagy, the Pho8Δ60 mutant protein is expressed. Pho8Δ60 localizes to the ...

...lung cancer cell line containing an activating mutation in epidermal growth factor receptor (EGFR) with...

...amplification can be used to create silent point mutations at internal recognition site(s) thus eliminating...

...potentially recombine. To account for this, alternate mutant versions of the loxP site have been created, which...

...now circulating and plasmids containing these mutations have been deposited at Addgene. Plasmids deposited...

...deletions, but CRISPR/Cas9 can also introduce point mutations. While using targeted nucleases to generate an...

...promoter of the 5’ long terminal repeat (LTR) is mutated in third generation systems such that it no longer...

...overexpression, knockdown (shRNA, miR-30-shRNA), mutation/editing (CRISPR-Cas9) or deletion (Cre), together...

... assays. ShadowG is darker than other dark YFP mutants, it reduces the risk of spectral contamination ...

...it’s also good to make a few single base pair mutations in crRNA target sites found within the homology...

...titration consider using a reverse transcriptase mutant in a separate control reaction. This will allow...

...seeing tRNAs because they contain “hard stop” mutations. New techniques used by the Lowe lab have allowed...

...to give the animal the full range of motion, a commutator allows the patch cable to connect to the laser...

... this model also displays the several Lys/Ala mutations reported by Ian Slaymaker and colleagues to reduce...

...chance of a chromosomal inversion, or just random mutations if the chromosome is repaired with all the original...

...Screening pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM) Pooled Library 80,962 1866 bp, plus...

... gene expression. Its catalytic domains can be mutated to inactivate the protein’s nucleolytic capability...

...possible to insert, or knock-in, any gene, tag, or mutated exon into the genome. For this purpose, the sequence...

...wouldn’t be a surprise if someone came up with a mutant NgAgo (or a novel endonuclease) protein that can...

... and Citrine) can be monomerized with an A206K mutation [2]. Addgene has many of these constructs and ...

...depositor has indicated that there is a particular mutation found within the gene/insert, this information...

...should also note that IRES and 2A peptides are not mutually exclusive elements. Labs have successfully utilized...

...tools, double dimerization GFPs, and circular permutation GFPs. Hopefully we will have new tools for plant...

...gates." Science. 340, 599-603 (3 May 2013). Vivek Mutalik et al. "Precise and reliable gene expression via...

...autoplay=False, hidden_controls=False, loop=False, muted=False, width='600', height='189', player_id='31668202421...

...and proteins in living plants, to complementing mutant phenotypes. Arabidopsis is amenable to the floral...

...adapted for a wide variety of applications - as mutagenesis tools for genetic studies (e.g. using mariner...

...Targeted Evolution. In this technique, a pool of mutant AAV capsid (cap) expressing plasmids is generated...

...namely that they can cause cancer via insertional mutagenesis (which HIV does not do). Furthermore, molecular...

...lucky socks, persevered through a nerve wracking commute complete with a disabled train, and now you’re ...

...transfer plasmids. The packaging plasmid carried a mutated HIV-1 provirus that couldn’t package itself due...

...defective phagemid; Bikard et al. did not observe any mutations at the target site. In a mouse skin colonization...

...column runs. The TEV protease itself, with various mutations used to increase its stability activity, can be...

...) and GRAB_NE1m (medium affinity), as well as GRAB_NEmut as a negative control. Find the sensors at Addgene...

...plasmid replication control that are not necessarily mutually exclusive. In the first, the replication initiation...

...Gather the resources you’ll need ahead of time and mute your notifications during. (Boyes, 2019) If you’...