We narrowed to 312 results for: Mut

1. New Tool for Lineage Tracing: The ClonTracer Library

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   Blog Post

   Published

   Sept. 22, 2015, 2:30 p.m.

   ...lung cancer cell line containing an activating mutation in epidermal growth factor receptor (EGFR) with...

2. Plasmids 101: Golden Gate Cloning

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   Blog Post

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   Aug. 27, 2015, 2:30 p.m.

   ...amplification can be used to create silent point mutations at internal recognition site(s) thus eliminating...

3. Plasmids 101: Cre-lox

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   Blog Post

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   Jan. 13, 2015, 3:47 p.m.

   ...potentially recombine. To account for this, alternate mutant versions of the loxP site have been created, which...

4. Plasmids for Endogenous Gene Tagging in Human Cells

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   Blog Post

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   April 6, 2017, 1:02 p.m.

   ...it’s also good to make a few single base pair mutations in crRNA target sites found within the homology...

5. Overcoming the Challenges of Lentiviral Production

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   Blog Post

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   Aug. 7, 2018, 12:31 p.m.

   ...promoter of the 5’ long terminal repeat (LTR) is mutated in third generation systems such that it no longer...

6. Popular Retroviral Vectors and Their Uses in Scientific Research

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   Blog Post

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   July 24, 2018, 12:51 p.m.

   ...overexpression, knockdown (shRNA, miR-30-shRNA), mutation/editing (CRISPR-Cas9) or deletion (Cre), together...

7. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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   Blog Post

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   May 15, 2018, 12:43 p.m.

   ... assays. ShadowG is darker than other dark YFP mutants, it reduces the risk of spectral contamination ...

8. Mouse Modeling, Part 1: Genetically Engineered Mice

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   Blog Post

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   July 11, 2019, 1:26 p.m.

   ...deletions, but CRISPR/Cas9 can also introduce point mutations. While using targeted nucleases to generate an...

9. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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   Blog Post

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   July 12, 2021, 1:15 p.m.

   ...now circulating and plasmids containing these mutations have been deposited at Addgene. Plasmids deposited...

10. Tips for Titering Your Lentiviral Preps

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    Blog Post

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    March 15, 2016, 2:30 p.m.

    ...titration consider using a reverse transcriptase mutant in a separate control reaction. This will allow...

11. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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    Blog Post

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    Dec. 1, 2015, 3:30 p.m.

    ...seeing tRNAs because they contain “hard stop” mutations. New techniques used by the Lowe lab have allowed...

12. The Materials Science of Optogenetics Experiments

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    Blog Post

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    Sept. 17, 2015, 2:30 p.m.

    ...to give the animal the full range of motion, a commutator allows the patch cable to connect to the laser...

13. 3D Printing Meets CRISPR Cas9

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    Blog Post

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    Oct. 17, 2017, 12:55 p.m.

    ... this model also displays the several Lys/Ala mutations reported by Ian Slaymaker and colleagues to reduce...

14. Truncated gRNAs for Regulating Gene Expression

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    Blog Post

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    Jan. 10, 2017, 3:37 p.m.

    ... gene expression. Its catalytic domains can be mutated to inactivate the protein’s nucleolytic capability...

15. Plasmids 101: Knockout/Knock-In Plasmids

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    Blog Post

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    Dec. 1, 2016, 3:30 p.m.

    ...possible to insert, or knock-in, any gene, tag, or mutated exon into the genome. For this purpose, the sequence...

16. Degrading DNA with Cascade-Cas3

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    Blog Post

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    Feb. 11, 2025, 2:15 p.m.

    ...chance of a chromosomal inversion, or just random mutations if the chromosome is repaired with all the original...

17. Pooled Library Amplifications

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    Blog Post

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    May 30, 2024, 1:30 p.m.

    ...Screening pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM) Pooled Library 80,962 1866 bp, plus...

18. Google Forums Round Up: First Impressions of NgAgo

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    Blog Post

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    Aug. 4, 2016, 2:30 p.m.

    ...wouldn’t be a surprise if someone came up with a mutant NgAgo (or a novel endonuclease) protein that can...

19. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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    Blog Post

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    April 19, 2016, 2:30 p.m.

    ... and Citrine) can be monomerized with an A206K mutation [2]. Addgene has many of these constructs and ...

20. Anatomy of a Plasmid Page at Addgene

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    Blog Post

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    Feb. 4, 2016, 3:30 p.m.

    ...depositor has indicated that there is a particular mutation found within the gene/insert, this information...

21. Plasmids 101: Multicistronic Vectors

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    Blog Post

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    Sept. 9, 2014, 8:20 p.m.

    ...should also note that IRES and 2A peptides are not mutually exclusive elements. Labs have successfully utilized...

22. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    Blog Post

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    June 12, 2014, 3:30 p.m.

    ...tools, double dimerization GFPs, and circular permutation GFPs. Hopefully we will have new tools for plant...

23. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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    Blog Post

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    Dec. 12, 2013, 3:35 p.m.

    ...gates." Science. 340, 599-603 (3 May 2013). Vivek Mutalik et al. "Precise and reliable gene expression via...

24. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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    Blog Post

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    July 16, 2020, 1:15 p.m.

    ...autoplay=False, hidden_controls=False, loop=False, muted=False, width='600', height='189', player_id='31668202421...

25. Transferable Skills Guide: Identifying Your Transferable Skills

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    Blog Post

    Published

    Jan. 11, 2018, 3:29 p.m.

    ...lucky socks, persevered through a nerve wracking commute complete with a disabled train, and now you’re ...

26. AAVs CREATed for Gene Delivery to the CNS and PNS

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    Blog Post

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    Sept. 28, 2017, 2:01 p.m.

    ...Targeted Evolution. In this technique, a pool of mutant AAV capsid (cap) expressing plasmids is generated...

27. Tips for arabidopsis transformation

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    Blog Post

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    Oct. 25, 2018, 1:23 p.m.

    ...and proteins in living plants, to complementing mutant phenotypes. Arabidopsis is amenable to the floral...

28. Viral Vectors 101: The Retroviral Lifecycle

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    Blog Post

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    July 27, 2023, 1:15 p.m.

    ...namely that they can cause cancer via insertional mutagenesis (which HIV does not do). Furthermore, molecular...

29. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    Blog Post

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    Dec. 7, 2020, 2:53 p.m.

    ...adapted for a wide variety of applications - as mutagenesis tools for genetic studies (e.g. using mariner...

30. Lentiviral Vector Uses and Overview

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    Blog Post

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    May 19, 2016, 2:30 p.m.

    ...transfer plasmids. The packaging plasmid carried a mutated HIV-1 provirus that couldn’t package itself due...

31. CRISPR Antimicrobials

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    Blog Post

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    May 3, 2016, 2:30 p.m.

    ...defective phagemid; Bikard et al. did not observe any mutations at the target site. In a mouse skin colonization...

32. Plasmids 101: Protein tags

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    Blog Post

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    Dec. 11, 2014, 4:26 p.m.

    ...column runs. The TEV protease itself, with various mutations used to increase its stability activity, can be...

33. Plasmids 101: Plasmid Incompatibility

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    Blog Post

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    March 31, 2020, 1:15 p.m.

    ...plasmid replication control that are not necessarily mutually exclusive. In the first, the replication initiation...

34. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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    Blog Post

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    Dec. 14, 2017, 2:08 p.m.

    ... This is to avoid the chances of insertional mutagenesis, which is the integration of transgenes into ...

35. Lab to Office Culture Shock

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    Blog Post

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    Feb. 5, 2019, 1:04 p.m.

    ...everyone has a voice and the group can come to a mutual agreement is an amazing thing to be a part of and...

36. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    Blog Post

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    Nov. 1, 2018, 12:35 p.m.

    ... a GFP cassette, and introduced site-directed mutations, all without introducing unwanted selection markers...

37. Plasmids 101: Codon usage bias

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    Blog Post

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    Sept. 27, 2018, 1:09 p.m.

    ...sometimes (but not always) be noted in in the “mutation” field on the plasmid page (see plasmid 87904 ...

38. Transferable Skills Guide: External Collaborations

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    Blog Post

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    April 19, 2018, 1:25 p.m.

    ...leverage another organization’s expertise to our mutual benefit.  Our partnership with SnapGene, for example...

39. Hot Plasmids - August 2020

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    Blog Post

    Published

    Aug. 18, 2020, 1:15 p.m.

    ...) and GRAB_NE1m (medium affinity), as well as GRAB_NEmut as a negative control. Find the sensors at Addgene...

40. Plasmids 101: Shuttle Vectors

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    Blog Post

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    May 20, 2025, 1:15 p.m.

    ...the researcher, Zeocin has been shown to cause mutations even in cells with the resistance gene, so it ...