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We narrowed to 602 results for: ACE

Showing: 301 - 350 of 602 results

  1. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ...to me. What’s on the fiber horizon? The brain interfaces with the entire body, so the obvious next applications...Flexible fiber-based optoelectronics for neural interfaces. Chemical Society Reviews, 48(6), 1826–1852. ...

  2. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...Incubate 5 Minutes at Room Temperature: You can place your reaction on ice if you are planning to transform...polymerases with proofreading functionality are used in place of Taq to reduce error rates; however, proofreading...

  4. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ...professorship. But recently a downward trend in funding and space in academic research labs has more students looking...industry, and government on social media (Twitter, Facebook, etc.) to learn about available jobs, fields, ...

  5. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ... our mindfulness group is sticking with their Headspace practice even while working remotely. Social distancing...reproducibility best practices in your research. For a place to start, check out Reproducibility for Everyone...

  9. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...movement. When the ribosome moves along an mRNA, it displaces other RNA-binding proteins to allow translation...translation to occur. For a TRICK reporter mRNA this displacement leads to a change in the fluorescent signal:...

  10. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ... hires initially based on those who notice and embrace the “odd and extreme openness” of the lab (i.e ...are engrossed in a task. Things seem to fall into place frequently and you sometimes feel confident in your...

  11. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ...legends who not only survive, but thrive, in the face of some of Earth’s harshest environments: corrosive...out rapidly in warm weather. Psychrophilic enzymes face very similar issues when forced to express in mesophilic...

  12. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ...information, however, did not improve at the same pace as the quantity and quality of the information. ...down, and zooming out — with an intuitive user interface.   The team approached the issue from multiple...

  14. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ...adjustments in the physical organization of your workspace and your workflow can have a huge impact. Test... experiments at odd hours, used more secluded workspaces, or politely asked to not be disturbed when they...

  15. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...endeavor. Due to the COVID-19 pandemic, students will face a reduced supply of internships as a result of the...narrative that is positive, and forward-looking. Embrace it, and let your personality leap out of the page...

  16. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ...amplified to add 5′ and 3′ homology regions to the adjacent fragment or vector. 5′ overhangs are generated...higher temperature at which Gibson assembly takes place may limit formation of secondary structures at the...

  17. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    Published
    Aug. 20, 2024, 1:15 p.m.
    ...can see from Figure 2 that determining where to place your gate based on the dot plot would be complicated...Conventional vs Spectral Flow Cytometry Beyond Surface Labeling in Flow Cytometry Resource on addgene....

  18. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...also sometimes called unnatural amino acids) are placed in a protein site-specifically to alter its function...using GCE in your experiments, GCE4All is a great place to start. They host monthly webinars, workshops,...

  19. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...focused on an emerging scientific field, BioBuilder embraces new opportunities for scientific exploration, ... opened in August 2017.  This flexible teaching space offers STEM programming, workshops, and customized...

  20. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    Published
    Feb. 4, 2014, 3:50 p.m.
    ...outside your organization. The mentor in your own workplace is able to: Help you identify specific skills...can tap for email discussions. It is also a great place to practice your mentoring skills. Online mentoring...

  22. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...understand so that we could possibly develop pharmaceutical interventions that would inhibit IL-36 release...intensity-based signaling biosensors to monitor intracellular signaling,” says Hébert. Previous research has...

  23. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ...communication, be it in your academic lab or your workplace, is an ongoing process. It is never really done...you try any of these approaches in your lab or workplace, let me know. If you have come upon any other ...

  25. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...Lancaster et al. (2018) Of course, preprints don’t replace journals and other modes of scientific communication...journal and share it more widely!  Not everyone embraces preprints as part of their scientific communication...

  26. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...entails will help keep you motivated when you’re faced with yet another hurdle in your research journey...will always be room to improve. The next time you face a problem, try to be mindful of how you work through...

  28. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...in recombinant AAVs (rAAVs) with maximum “trunk” space for genetic cargo. Typically, when researchers speak...dependent switches control gene expression via switches placed in your cell type of interest. Careful combination...

  29. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ... RNA editing. Fast facts – PAM requirement: protospacer flanking sequence – A, U, or C. Best for: targeting...development and use. Fast facts – PAM requirement: protospacer flanking sequence – none. Best for: targeting...

  31. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ... Viruses are intracellular parasites and natural vehicles for genetic information. Therefore they make....9 kb stuffer sequence that can be removed and replaced with an shRNA sequence of your choice. More information...

  33. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...SARS-CoV-2 virus depends on its spike protein to bind ACE2, the host cellular receptor. To more easily study...SARS outbreak, Hyeryun Choe’s lab identified that ACE2 is the functional receptor for SARS-CoV. The lab...

  35. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...from the drop-down and add an explanation in the adjacent Patent/License Explanation field. Filling ...in the format: labname_addgene_batch_upload.xls Replace ‘labname’ with the name of your PI and send the...

  36. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...co-founder of www.assaygenie.com. No matter what facet of academia you are in, grant writing can be the...clear that you are staying because it is the best place in the world for your research to rapidly progress...

  37. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...Google it...Or Yahoo! it, or Bing it, but, let’s face it, there’s a reason nearly 70% of all internet ...experiment, but a picture of a gel or a sequencing trace file can go a long way in helping us understand ...

  38. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...gene cassettes. Thse vectors are engineered to replace the drug resistantance genes in the bacteria thus...known antibotics. These might be used on hospital surfaces and in hand sanitizers.  There are many clever...

  39. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ... scatters a great deal once it enters tissue, placement above the site of stimulation guarantees that ...brain tissue a short distance so ideal implant placement should be about 0.4-0.5mm above the site of your...

  40. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    Published
    Aug. 6, 2015, 2:30 p.m.
    ...teamwork, manage day to day operations within their workplace, and pursue leadership opportunities at the local...postdoc). I soon realized that many of the issues faced by postdocs and institutions are not local but are...

  41. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...conferences, but after receiving her degree she was faced with a similar conundrum – she had the skills, training...color changing flower project was inspired by a Facebook comment.  In our day job, we worked on plants ...

  43. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...so many technologies currently available, you now face the challenge of trying to figure out what system...Scientific Inc., Origene, and NEB® are all good places to look for some helpful kits! Efficiency: Wouldn...

  44. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ...comfortable with awkward silences but I’ve learned to embrace them after one ended with the person in front of...your manager for advice if you’re not sure how to face a particular situation. But keep in mind that a ...

  45. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ..., but those outside of your field can point out places where you gloss over essential details or omit ...avoid rushing out the door without your poster, place it by your keys a day in advance. Dress for the ...

  46. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...is typically purified using methods like ultracentrifugation, while lentivirus supernatants are centrifuged...collection 2–4 days 5–10 days Purification Ultracentrifugation (AAV); centrifugation (lenti) Centrifugation...

  47. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...applications across a wide range of topics. He embraces open science, self-publishing directly on his .... R., Evans, E. W., Déjean, V., Dodson, C. A., Wallace, M. I., Mackenzie, S. R., Timmel, C. R., & Hore...

  48. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...simply as it seems laid out above, there are many places where the PhD application cycle becomes much more...program will allow for you to have the time and space to delve into what matters to you. It can also be...

  50. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...track protein localization, cell structure, intracellular trafficking, and protein turnover rates. Additionally...overexpressed fluorescent protein fusions is to replace the genomic copy of your gene of interest with ...
Showing: 301 - 350 of 602 results