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We narrowed to 609 results for: CaS;

Showing: 301 - 350 of 609 results

  1. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...will be blocked during protein folding, in which case you should try tagging the opposite end.  If neither...disrupted by denaturants (Hochuli, 1988). In some cases, proteins can be bound under denaturing conditions... aren’t any good antibodies available. In these cases, having a tagged version of the protein is hugely...

  2. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...cytometry, secondary antibodies may be indicated on occasion, usually after other signal amplification techniques...indiscriminately, giving them the same output signal. In these cases, it is best to use the direct method when possible...confidently, collect a broader array of data, and occasionally ask themselves and others that most perplexing...

  3. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ...' decisions to alter postdoc salaries - in some cases reversing these decisions entirely. At Future of...or are being compelled at the state level, in the case of some public institutions) to cancel plans to ...communication of their plans (many postdocs - and in some cases, faculty - have found out about what their institution...

  4. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ...entirely, and our software needs to handle these cases. The following code examples show how software can...sample and get it ready for shipping. In the first case, we naively assume that everything will go perfectly...we can interchange them easily as needed. In this case, the physical considerations of the laboratory make...

  5. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ...single 72h harvest is best for larger genes such as Cas9. See figure 1 for an example of how changing transfection...enough to get a sufficient titer; this is often the case with large genes. This is due to inefficient packaging...inserts; a study by Kumar et al. found that in some cases, titers can drop 10-fold for every 2kb increase ...

  6. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    Published
    Aug. 6, 2019, 12:55 p.m.
    ...those used for breeding heterozygous mice. In this case, your parental mouse should be hemizygous at the...similar epigenetic modifications. Special breeding cases Whether your mouse colony consists of genetically...explained more in Part 1 of this series). In any case, Cre is a dominant phenotype, while you’ll want ...

  7. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...your gene of interest.  SapTrap Tool Kit for CRISPR Cas9 edits At Addgene, we have the entire SapTrap toolkit.... "SapTrap, a toolkit for high-throughput CRISPR/Cas9 gene modification in Caenorhabditis elegans." Genetics...blog Learn more about SapTrap Read about CRISPR/Cas9 in C. elegans Browse our Plasmids 101 blog posts...

  8. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...other things, science communication comprises broadcasting, science writing, and certain aspects of art...audio or visual person. Why don’t you record a podcast or put some effort into making a video? Use your...Communication Posts Browse our Career Page Listen to Our Podcast Episode on Science Communication Additional Resources...

  9. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...they’re knowingly selling a bad product (in which case, you should probably stop using that company). Frequently...frequently asked by customers (and I expect this is the case at most companies). Always go back to the source...that could take weeks to resolve adequately. Occasionally there are some really off-the-wall questions...

  10. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...endoplasmic reticulum localization, Munro and Pelham casually introduced the Myc-tag (Munro, et al. 1986; Munro...developed almost 40 years ago? Well…kinda. In some cases, antibody providers are distributing monoclonal ...same hybridoma developed by Evans, et al. In other cases, providers have moved to producing anti-Myc [9E10...

  11. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ... matches the copy number of your sample. In that case, an RQ value of one (or, more realistically, between...ddPCR may be a better option for more challenging cases.  Price point If budget is a concern, look for...number and it may not be able to identify trends in cases where the copy number variation is two or less (...

  12. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...always maintain a backup replicates of each clone in case of contamination. We do not normally use antibiotics...well plate for further analysis. To assess CRISPR/Cas9 knockout efficacy, we have used both Western blotting...potentially large number of single clones obtained (on occasion we have prepared two 48-well plates of clones)...

  13. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...individual expression cassettes, having the genes expressed from the same cassette is sometimes advantageous...and you can then insert the whole multicistronic cassette into a backbone as a single unit. Although it ...

  14. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...compared the number of plasmid orders and new COVID-19 cases for each week by location. We observed that as expected...drop dramatically when there are many new COVID-19 cases being reported (right, red), likely due to quarantining...map showing the plasmid orders and new COVID-19 cases each week. Statistical Modeling Note -- If statistical...

  15. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...options available to today's science communications: podcasts, blogging, videos, lectures, exhibits, social ...content.  What are you interested in? Math, sarcasm, romance, and spacecraft! Image courtesy of xkcd.com...can increase exponentially with complexity. Podcasts Medium Easy for audience to engage while multitasking...

  16. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...worry about vector replication at all. In these cases, transient transfection may be sufficient for your...transfection experiments. Reporters Finally, in some cases a researcher may choose not impose selection on ...organism your experiment takes place in. In many cases, you won’t need to worry about the expression of...

  17. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    Published
    Aug. 13, 2019, 12:58 p.m.
    ...zinc-finger nucleases, TALENs, and more recently CRISPR/Cas9. Another popular targeted gene deactivation method..., relying on established reagents such as CRISPR/Cas9, Tol2, and the high-quality sequenced zebrafish ..., and their resistance (so far) to facile CRISPR/Cas9-mediated insertion of foreign DNA. Despite these...

  18. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ...Alongside there is a second-line team that takes on cases requiring in-depth analysis, data review, in-house...knowledge and judgement. For particularly tricky cases, team discussions can often lead to otherwise unobtainable...patient, attentive, empathetic, and tactful. Occasionally it can also be necessary to take the lead and...

  19. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...applications, such as protein isolation from serum. In this case, Protein A and Protein G will bind both the primary...indiscriminately, causing competition for binding sites. In cases like these, antibodies can be directly conjugated...simply isn’t available for a specific target. In cases such as this, an epitope such as c-Myc, GFP or V5...

  20. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...approach known as multimodal therapy. Sadly, in many cases the cancer is diagnosed so late that these treatments...success in treating mesothelioma, such as in the case of Paul Zygielbaum. A different approach to immunotherapy...and used throughout the world. New mesothelioma cases could be greatly decreased through a ban on asbestos...

  21. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...their data months or years before others. In some cases, typically consortia involving genomic screens, ...the originating researchers, even if they are occasionally beaten to press, as there is so much analysis...track the motion of multiple animals on different occasions via video, generating huge amounts of data. When...

  22. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...in lentiviral or retroviral backbones. On some occasions, shRNAs are transiently expressed using either...promoters with no tissue specificity, so in some cases they can lead to toxicity and off target effects... an Old Favorite CRISPR 101: Targeting RNA with Cas13a (C2c2) Plasmids 101: The Promoter Region – Let'...

  23. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    Published
    Sept. 1, 2020, 1:15 p.m.
    ...complement each other to restore the full-expression cassette. AAV’s natural ability to reassemble fragmented...sequential homology directed repairs (HDR) with CRISPR/Cas9. Donor A contains: 400bp homology arms to the site...2017) CRISPR-Mediated Integration of Large Gene Cassettes Using AAV Donor Vectors. Cell Reports 20:750–756...

  24. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ... an intact sample, a cell in the case of ICC or a tissue in the case of IHC. IF is used specifically when...interest within an intact cell, tissue, or, in some cases, a whole organism. But, as with any antibody-based...

  25. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...(Mouse IgG2a) Goat anti-mouse IgG2a CASPR anti-CASPR mAb K65/35 (Mouse IgG1) Goat anti-mouse...Neocortex labeled with anti-pan-Nav (magenta), anti-CASPR (green), and anti-Kv2.1 (cyan). (B) Cerebellum labeled...

  26. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...plasmid with repeating sequences. In some specific cases, multimerization has been observed to occur more...reduce the frequency of multimerization, and in some cases completely prevent the formation of plasmid multimers...Sharratt, 1984; Summers et al., 1993). In these cases, screening multiple bacterial colonies using one...

  27. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...environmentally friendly organization on the planet, in which case whoa! Mission accomplished! One example of a mission-based... a little bit about everything or in Jefferson’s case, even a lot about everything. Fast forward a few...received 104 million results (Here’s the top one, in case you’re interested). “But I’m an introvert!” is not...

  28. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...can alter the structure of the protein. In some cases this can lead to changes or a complete loss of protein...lipids including those of the cell membrane. In some cases, this can mask epitopes such that immune complexes...only enter cells with disrupted membranes as is the case with dead or dying cells. DNA binding dyes are not...

  30. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    Published
    Feb. 4, 2015, 3:58 p.m.
    ...the dramatic spread of the Cas9 technology: "The rapid adoption of the Cas9 technology was also greatly...

  31. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ..., and bidirectional transport for AAV1, -8, -9 (Castle et al., 2014). Differences between AAV serotypes...space along an axon, this does not seem to be the case for either AAV1 or AAV9. It should be noted that...PMID: 25555356. PubMed Central PMCID: PMC4324159. Castle, M.J., Gershenson, Z.T., Giles, A.R., Holzbaur,...

  32. The Twelve Days of CRISPR

    Type
    Blog Post
    Published
    Dec. 22, 2020, 2:15 p.m.
    ...of CRISPR, Addgene gave to me: RNA editing with Cas13.  On the tenth day of CRISPR, Addgene gave to me...to me: methods for adenoviral delivery of CRISPR/Cas9.  On the twelfth day of CRISPR, Addgene gave to ...

  34. Editor's Choice, October 2016

    Type
    Blog Post
    Published
    Nov. 4, 2016, 2:30 p.m.
    ...Community.” This post leads readers to our latest podcast all about graduate student life and how establishing...Community Engineering the Plant Genome Using CRISPR/Cas9 Healthcare Consulting: A Door to the Business of...

  35. Grad School Advice Part 1: Picking a Lab and a Project

    Type
    Blog Post
    Published
    Sept. 1, 2016, 2:30 p.m.
    ...and a project. Listen to the interview here! Podcast Breakdown (Time : Topic) 0:00 - 0:28 : Niroshi ...the audio engineer and producer for the Addgene Podcast.     Additional Resources on the Addgene Blog ...

  36. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    Published
    Feb. 13, 2014, 1 p.m.
    ...contains and other important information. Tip: A lowercase "p" is often used as the first letter of a plasmid...pBACKBONE-hGene Tip: Often researchers will add a lower case letter to the beginning of their insert abbreviation...

  37. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ...World Health Organization says the number of new cases could rise to 10,000 a week within two months. ...has been fatal in 70 percent of the most recent cases, primarily in West African countries. Even that ...

  40. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    Published
    Jan. 17, 2018, 2:57 p.m.
    ...injection site and choosing a specific serotype, but occasionally you may want to further control where your gene...mouse line that contains a Cre-dependent mCherry cassette under the control of an otherwise ubiquitous promoter...

  41. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...uses catalytically inactive CRISPR endonuclease (dCas9) fused to a deaminase to make the transitions at...Viral Transductions Single Base Editing with CRISPR Cas9 Activators: A Practical Guide 10 Steps to a Perfect...

  43. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    Published
    Nov. 14, 2016, 3:30 p.m.
    ...positively affect scientific research. As Maria Castro of University of Michigan says, “Addgene has transformed... helped accelerate scientific discoveries.” Occasionally, a plasmid tool is so influential that it is...

  44. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...genes at the site of insertion. In both of these cases, transposons combine the advantages of viruses and...2) a transposon containing the gene-expression cassette that can translocate within the genome. The Sleeping...

  45. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    Published
    March 31, 2015, 4:08 p.m.
    ... the other posts in this series. Listen to our podcast interview with Connie Cepko to learn about her ...leaders rarely give orders (although they must occasionally). Good leaders exert influence in other ways...

  46. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ... a PI who is a good mentor is important in both cases. As a PhD student you need to learn how to be a ...you pursue any career alternative? Listen to our podcast interview with Harvard Medical School researcher...

  47. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ... is not suitable for use in apoptotic cells as Caspase3/7 both cleave after the DVPD sequence. Check out...protein functionality (Arribere, et al). In rare cases, tags can also affect protein localization and solubility...

  50. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...transgene free individual. For example, with CRISPR-Cas9 gene editing, transformation may be identified by...CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9 Tips for Arabidopsis Transformation CRISPR 101: ...
Showing: 301 - 350 of 609 results