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Showing: 301 - 350 of 398 results

  1. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...you take the plunge – plants are the foundation of life on earth, and opportunities abound to build better...

  3. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...commonly rely on RNA isolation kits to make their life easier. Recently, we published a blog on DNA purification...

  5. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...being Addgene. You guys rock." — Andrew York, Calico Life Sciences. Most of all, we’re incredibly thankful...

  6. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ...the lack of an Fc region. The lack of Fc also simplifies analyses when used in structural studies and ...

  7. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...These properties allow for efficient signal amplification, making biotin conjugated antibodies good options...

  12. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...uses these components as part of its natural viral life-cycle, and researchers have adapted the components...

  13. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...website, the new version of which will feature a simplified user interface, expanded numbers of curated molecules...

  21. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    .... In short, the Fragmid system takes the heavy lifting out of CRISPR vector design, making it easier to...

  25. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...quantify the amount of PCR product that comes from amplification of the original plasmid which can over-inflate...

  37. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ... an enhanced pU6cm promoter, viral replicon amplification of cargo, and heat treatment.  Figure...

  38. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ...a manuscript? And that’s only in your scientific life! I’m certain that you also negotiate with friends...

  40. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...and not another genetic disease more prevalent or life-threatening (cystic fibrosis, hemophilia, muscular...

  43. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...directly into a PCR buffer, where DNA or RNA can be amplified. Compared to a commercial magnetic bead-based ...

  47. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...plant genome using CRISPR/Cas9 Using pSiM24 for simplifying plant genetic engineering Using arabidopsis in...
Showing: 301 - 350 of 398 results