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Showing: 121 - 140 of 440 results

  1. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...at this business and started things up for real: bank account, business cards, incorporation, laboratory...this transition (so far) and what we’ve learned.  Thank you to our guest bloggers! Nikolai Braun grew up...

  2. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...provide them with a CV or transcripts if requested Thank them, and eagerly await a reply. It’s a good idea...interest. Benefits of Online and Hybrid Options Thanks to the much more developed virtual environment ...

  3. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...cells. These plasmids use fluorescent proteins flanked by long regions of homology to the gene of interest...and protein-protein interactions. Additionally, thanks to the modular arrangement of vectors included ...

  4. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...plasmid that contains the Mobile CRISPRi cassette flanked by left and right Tn7 end sequences. Donor bacteria...in the recipient cell, the CRISPRi DNA (purple) flanked by left and right Tn7 end sequences (green) integrates...

  6. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...alterations are achieved through DNA degradation, thanks to the unique capabilities of Cas3 and its partner...CRISPR type I-E. Created with BioRender.com. Thanks to this complex, developing Cas3 tools has been...

  7. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...because of our CRISPR depositors and requestors. Thank you!  Want to dive deeper into Addgene’s CRISPR ... The CRISPR Journal (Pyhtila et al., 2023). Our thanks to Rodolphe Barrangou for his authorial contributions...

  8. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...transmitting and sensing electrical potential. Thankfully, there is a tool to measure these electrical ...specific types of cells, such as neuron subtypes, thanks to viral serotype specificity during delivery (...

  9. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ... spent organizing, an hour is earned (Benjamin Franklin)! It’s good practice to label your plates and/...autoclaved before being disposed Happy culturing! Many thanks to our guest blogger, Sana Khan Khilji! Sana Khan...

  10. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...depositors to provide plasmid maps whenever possible. GenBank files can also be uploaded onto our plasmid pages... resources provided by the depositor including genbank files and protocols here. Articles Citing this ...

  11. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...green fluorescent protein (GFP) (6).   The Dodt and Frank Bradke laboratories later developed the 3DISCO (... system under normal and diseased conditions.  Thank you to our guest bloggers, Jae Lee and Pantelis ...

  12. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ...and single guide RNAs (sgRNAs) with appropriate flanking homology into a germline cell can create an RNA-guided...drives can be found in the referenced publications. Thank you to our guest blogger! An inventor of technologies...

  13. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...capsid, as the engineered, recombinant genome is (thankfully) gutted of many essential viral genes. How it...blog was written by Addgenie Brian O'Neill, with thanks to Addgenie Rebecca Hutcheson for her assistance...

  15. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...know how it goes! Acknowledgements: I’d like to thank the PTA of Butler Elementary for trusting me with...with Math & Science Night, and a special thanks to Joanne Marks, Jonathan Marks, and Vanessa Bullard of...

  16. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...transfer plasmid encoded the desired transgene flanked by HIV-1 long terminal repeats (LTRs) that facilitate...Bernaudin F, Girot R, Dorazio R, Mulder GJ, Polack A, Bank A, Soulier J, Larghero J, Kabbara N, Dalle B, Gourmel...

  17. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...These primers are designed to anneal to sites that flank the insert site. A positive clone will produce a...this type of primer pair anneals to a sequence flanking the insert and one primer anneals to the insert...

  19. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...in-vivo biotinylation signaling peptide (Bio), flanked by hexahistidine motifs (6xHis) MBP Large Protein...Expression Vectors from the Berkeley QB3 MacroLab Eric thanks his wife, Annette Sievers, Ph.D., for her tips ...

  20. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...Cas13 enzymes require a single base protospacer flanking sequence (PFS) adjacent to the target, but many...018-1591-1 Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F (2017) RNA editing...
Showing: 121 - 140 of 440 results