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  3. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ...). Bacteria need special fertility genes to be capable of conjugation, and these are encoded by certain...conserve energy, these specialized degradative capabilities are often tightly regulated to be active only...

  4. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...you’re simply attending, but uncertain how to capitalize on your experience. No matter the reason for ...bullet points and elaborate if your audience seems captivated. Expertly navigate questions Answering questions...

  5. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...thin sheet to illuminate a fluorescent sample and captured the reflected light using a different objective...Ultramicroscope was developed using a stereomicroscope to capture large field-of-views (6). For more comprehensive...

  6. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...would permit point mutations in RNA which could recapitulate or rescue known pathogenic alleles, or introduce...REPAIRv1 to merely 20. Expanding RNA Editing Capabilities through RESCUE Two years later, the Zhang lab...

  7. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...rough "COVID-19 effect" on order data, it does not capture the fact that different parts of the world encountered... each paper, respectively. The fitted model captures weekend, Christmas and New Years, and COVID-19...

  8. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...hydrophobic glass filter. Each glass filter can capture up to 150 ug of DNA, and filters can be stacked...stacked together in series to increase binding capacity. Figure 1: Purify DNA using glass filters. Image...

  9. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...likely do! Give our summary table a look for a quick recap of the pros and cons of each antibiotic and happy...novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins ...

  10. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...Advantages of lentivirus include a large genetic capacity and the ability to transduce both dividing and...for applications that require the large genetic capacity of a lentiviral vector but for which transient...

  12. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    .... Since target specificity is determined by the capsid of the AAV, many of the vectors in Addgene’s catalog...including the retrograde AAVrg and the systemic capsid PHP.eB.   Figure 4: A) Cumulative AAV preps...

  13. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...integration of cargo without using screening markers. Capability to construct a strain library with different ... that same year. Her research is focused on the escape mechanism of genome editing in microorganisms which...

  14. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...areas associated) compared to an animal running to escape a threat. Locomotion can thus be used as a readout...behaviors are possible: active swimming in attempts to escape, or passive floating on the water surface. The ...

  15. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...of drugs, and developing internal operational capabilities. In just two years as a healthcare consultant...equity research firms, biotech start-ups, venture capital firms, and more. Essentially any role in the broad...

  16. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...with streptavidin, the lateral flow strip first captures the streptavidin portion of the probe via biotin...probe that is cut will continue to flow and be captured by an antibody further along on the strip. Highlights...

  17. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...variability, and in some cases, counter-selection escape can occur: single crossover colonies can acquire... the toxin itself (leading to counter-selection escape). The rhamnose induction system is well-characterized...

  18. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...study diseases such as cancer since mice better recapitulate the complex interactions between cancer cells...studied in other model organisms that better recapitulate in vivo conditions similar to humans. For example...

  19. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...major equipment can be defined as equipment with capital costs greater than $5,000 but this can depend on...like pipets, or mini-centrifuges, etc. Be sure to capture as many of these tools as possible, this even includes...

  20. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...refers to the different protein compositions of AAV capsids, which determine how a virus enters the cell. Different...Manfredsson, F. P. (2017). Rationally Engineered AAV Capsids Improve Transduction and Volumetric Spread in the...
Showing: 121 - 140 of 332 results