We narrowed to 149 results for: CO
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TypeBlog PostPublishedOct. 13, 2022, 1:45 p.m.... activation domains to the same genetic locus via co-expression of an epitope tagged dCas9 and antibody-activator...
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Hot Plasmids: Fall 2024
TypeBlog PostPublishedNov. 21, 2024, 1:30 p.m.... fluorescence markers like GFP (510 nm), allowing co-imaging. And because bioluminescent markers do not... -
Tips for CRISPR Gene Editing in Mice
TypeBlog PostPublishedJune 28, 2016, 10:59 a.m....BbsI site. (c) The pCAG-EGxxFP target plasmid was co-transfected with pX330 plasmids containing sgRNA ... -
Harnessing Bacterial Toxins for Allelic Exchange
TypeBlog PostPublishedAug. 15, 2019, 12:30 p.m.... allow stronger counter-selection than one merely co-opted toward that aim (i.e. sacB). Importantly, to... -
Five Popular Model Organisms
TypeBlog PostPublishedApril 11, 2019, 12:39 p.m....Saccharomyces cerevisiae to human: The important gene co‑expression modules." Biomedical Reports 7.2 (2017... -
Plasmids 101: Biotinylation
TypeBlog PostPublishedNov. 15, 2018, 1:50 p.m....biotinylated (more on this below) it can be incubated or co-expressed with avidin or a structurally similar protein... -
Quick Guide to Near-Infrared Fluorescent Proteins
TypeBlog PostPublishedOct. 5, 2021, 1:15 p.m....exogenous BV and after normalization to fluorescence of co-transfected EGFP. e Originally reported as a monomer16... -
CRISPR 101: Any Base Transversion Editors
TypeBlog PostPublishedApril 1, 2025, 1:15 p.m.... activity. To drive selective A → T editing, they co-expressed Polη, a translesion synthesis polymerase... -
Antibodies 101: Beyond Surface Labeling
TypeBlog PostPublishedJune 27, 2024, 1:15 p.m....killer”) cells. Typically, you would label the CD8 co-receptor protein on the surface of the CD8+ T cells... -
Hot Biosensors 2022: Year-End Roundup
TypeBlog PostPublishedDec. 6, 2022, 2:15 p.m.... (ITAM). By fusing ITAM to an RTK of interest and co-expressing a cytosolic ZtSH2 reporter, they observe... -
Using Phosphoserine to Study Protein Phosphorylation
TypeBlog PostPublishedJune 23, 2016, 2:30 p.m....type’ control can be made one of two ways. First, co-expression of a suppressor tRNA (SupD) instead of... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog PostPublishedApril 24, 2015, 2:39 p.m....each due to size limitations associated with AAV; co-infection with both constructs produces a minimum... -
Mycoplasma Contamination: Where Does It Come From and How to Prevent It
TypeBlog PostPublishedOct. 8, 2020, 1:15 p.m....doi.org/10.1290/1071-2690(2002)038<0086:caeomi>2.0.co;2 Uphoff CC, Drexler HG (2011) Detecting Mycoplasma... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog PostPublishedJune 24, 2020, 5:45 p.m....targeted cytosine methylation in mammalian cells. Co-expression markers EGFP and PuroR enable sorting ... -
Technologies Enabled by NanoLuc® Luciferase
TypeBlog PostPublishedFeb. 8, 2018, 12:17 p.m....design a better fluorescent protein that could be co-imaged with GFP using the same wavelength for excitation... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog PostPublishedMay 23, 2023, 1:15 p.m....interesting protein targets and you want to know if they co-localize at the same place in the cell? Or you know... -
Recombinase-based State Machines Enable Order-dependent Logic in vivo
TypeBlog PostPublishedJuly 28, 2016, 2:30 p.m....below). In the absence of extra recombinase-specific co-factors (which should be excluded from the system... -
Antibodies 101: Introduction to Antibodies
TypeBlog PostPublishedJan. 19, 2021, 2:15 p.m....production of functional scFv antibody in E. coli by co-expressing the molecule chaperone Skp. Front Cell... -
Transferable Skills Guide: Teamwork
TypeBlog PostPublishedNov. 28, 2017, 2 p.m....joined Addgene’s Green Team, a like-minded group of co-workers who want to make our organization as environmentally... -
Viral Vectors 101: AAV Variables That Matter
TypeBlog PostPublishedJune 1, 2023, 1:15 p.m....serotypes utilize a number of different receptors, co-receptors, and other mechanisms to gain entry into...