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We narrowed to 148 results for: Coli

Showing: 121 - 140 of 148 results

  2. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...construct can be easily expressed and purified from E. coli, retains important functional properties, and is...

  4. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...PCR reaction, no ligation is required since the E. coli you transform your PCR products into will efficiently...

  5. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...post: Synthetic Photobiology: Optogenetics for E. coli. Schmidl, et al. ACS Synth Biol. 2014 Nov 21...aggregation propensity in four expression systems: E. coli, wheat germ, HeLa, and Leishmania (Gagoski, et al... replication increases plasmid copy number in E. coli. The authors used pXPG to study the human GM-CSF... F30-2xdBroccoli are available, as well as 5S RNA F30-2xdBroccoli fusion and pET28c-F30-Broccoli controls...mammalian cells with Broccoli Vegetable tags, including Spinach, Spinach2, and Broccoli, can benefit your...scaffold allows insertion of two dimeric Broccoli tags (2xdBroccoli) to further enhance fluorescence of the...controls. Don’t just eat more broccoli, use Broccoli tags in your experiments too! Filonov, et al. Chem...

  8. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...Engineering production of functional scFv antibody in E. coli by co-expressing the molecule chaperone Skp. Front...

  11. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...Bacterial Expression Construct SpCas9 Inhibition (E. coli) Mammalian Expression Construct SpCas9 Inhibition...

  12. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ... respectively. Both Red Broccoli and Orange Broccoli are derived from Broccoli, and share similar properties...properties to Broccoli despite binding to a different small molecule. Notably, like Broccoli, Red Broccoli and ...was described in the same study as Red Broccoli and Orange Broccoli, two more aptamers that bind DFHO and...collection. Green fluorescent aptamers Spinach and Broccoli and their derivatives are aptamers able to bind...Cas9 and track the localization of the complex. Broccoli, the other green fluorescent aptamer, was identified...brightest fluorescence (Filonov et al., 2014). Broccoli is twice as bright as Spinach2 when bound to DFHBI...useful tool for in vivo studies. Dimerization of Broccoli increases its fluorescence by about 1.8-fold, ...

  13. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...of the E.coli BPL, BirA. If you recall, BirA biotinylates only one lysine in the entire E.coli proteome...in the entire proteome that are biotinylated. In E.coli for example, the BPL, BirA, is responsible for ...

  15. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...PubMed PMID: 24882160. Creed, Meaghan, Vincent Jean Pascoli, and Christian Lüscher. "Refining deep brain stimulation... Glutamatergic synaptic plasticity in the mesocorticolimbic system in addiction. Front Cell Neurosci. ...

  16. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    Published
    Dec. 31, 2019, 2:26 p.m.
    ...small molecule, are often named after vegetables; Broccoli, Spinach, and Corn, for example. Learn more about...

  20. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...Assembled plasmids can be directly transformed into E.coli for plating, screening and generation of sgRNA....
Showing: 121 - 140 of 148 results