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We narrowed to 553 results for: ESP

Showing: 121 - 140 of 553 results

  1. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...facilitate binding and cleavage of target DNA respectively. LshCas13a, on the other hand, uses only a short...(RPA) or Reverse-transcription RPA (RT-RPA), respectively. Amplified nucleotides are combined with Cas...expression. Although the collateral RNA cleavage response is well described in bacterial cells, LwaCas13a...

  2. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...Antibiotic resistance is defined as microbial survival despite exposure to antibiotics designed to kill them or...health of the patient as quickly as possible, especially if the infection is serious or life-threatening...around the disk is measured in millimeters, and corresponds to the level of susceptibility you might expect...

  3. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...through video     Video abstracts about research, especially published research, have grown in popularity ...turning video views into online article downloads – especially if they are published in open access journals...studies on reducing antibiotic prescriptions for respiratory tract infections. The video abstracts provide...

  4. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type
    Blog Post
    Published
    July 23, 2019, 12:46 p.m.
    ... Wilson Lab also looked for the gene variant responsible for the PHP.B permissive phenotype in mice. They...transfer to the rat CNS: AAV PHP. EB vector dose–response and comparison to AAV PHP. B." Gene therapy 25.5...Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain." Nature biotechnology...

  5. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...Quiñones-Valles, C., Espíndola-Serna, L., Martínez-Antonio, A., Quiñones-Valles, C., Espíndola-Serna, L., & Martínez-Antonio...Solar, G., Giraldo, R., Ruiz-Echevarría, M. J., Espinosa, M., & Díaz-Orejas, R. (1998). Replication and...

  6. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...exhibit slow head growth, develop seizures, cardio-respiratory dysfunction, distinctive hand movements, and ...MECP2 duplication syndrome or Rett Syndrome, respectively. One of the main challenges facing Rett researchers...Gray’s work, are each expected to advance their respective gene therapies to clinical trials in 2021. ...

  7. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...training It’s hard to recall a lot of details, especially when you are in a new place, learning something...normal and very helpful; don’t be afraid to ask! Especially for those new to the bench, remember to let your... expect them to work around your schedule, be respectful of theirs! Show up prepared and take training...

  8. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ..., but they did so without a substantial immune response or toxicity. More AAV-based CRISPR systems Smaller...with AAV, at just 1,108 and 986 amino acids, respectively, the size of both is within AAV’s packaging ...A multifunctional AAV–CRISPR–Cas9 and its host response. Nat Methods 13:868–874 . https://doi.org/10.1038...

  9. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    Published
    Feb. 18, 2020, 2:15 p.m.
    ...when bacterial cells regulate gene expression in response to changes in cell density. At the mixer, they...reviewing and commenting. Binning Singletons is especially grateful to its mentors and supporters during...was that kid who used recycled notebook paper, despite the fact that it was gray. He has worked in molecular...

  10. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...but simply whether its concentration changes in response to experimental parameters. This is a job for ... Figure 5: An example gel (left) with bands corresponding to points on the plot of protein abundance versus... Indeed, the quantification in panel B is not especially linear. In panel C, two different methods of ...

  11. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...it is for some people, especially women), visibility (how to get it, especially if introverted ), and ...fits in the bigger science picture. Scientists especially like to learn new things, so rehash common knowledge...

  12. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...release factor 1, the protein responsible for terminating translation in response to the presence of TAG sequences...organisms lacked a classic cysteinyl tRNA synthetase, despite abundant incorporation of cysteine in their proteomes...

  13. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...similar levels of fluorescence when bound to DFHBI despite being approximately half the length (Warner et ...and fluoresce in the red and orange channels, respectively. Both Red Broccoli and Orange Broccoli are derived...Broccoli, and share similar properties to Broccoli despite binding to a different small molecule. Notably,...

  14. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...extra wet lab work, this is still a great option, especially if the options outlined above aren’t realistic...reduced off target editing, such as SpCas9-HF1, eSpCas9(1.1), or HypaCas9. Read more about Cas9 options... your editing experiment not go to plan? Don’t despair, you have options! If you think delivery of Cas9...

  15. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...even see different sets of bands on the gel corresponding to each type of multimer present in your sample...dimer band can sometimes overlap with the band corresponding to the nicked or relaxed conformation of the...Uncut DNA (lane 2) has a band at <3 kb, which corresponds to the supercoiled plasmid monomer, and at <6...

  16. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    Published
    Nov. 6, 2015, 3:30 p.m.
    ...connecting international experts and students. In response to such needs, the International Mentorship Program...progress video conferences and had real-time correspondence via e-mail throughout the entire year. In addition...program, evaluated survey results. Participants’ responses to the mid and post-program self-assessment revealed...

  17. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    Published
    Aug. 5, 2025, 1:15 p.m.
    ...defined environmental conditions. These methods are especially powerful when coupled with iterative rounds of... antibodies with verifiable data is critical, especially when sharing with other scientists. Addgene partners...synthetic antibody products and development methods. Despite recent advances, there is still always room to ...

  18. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...position, what will you need? Multiple first or corresponding author publications (published, not in preparation...audiences and if you pay attention to how the audience responds to your presentations, you will become a more ...experience and learn about modern pedagogy techniques, especially active learning. Serve as a daily mentor for ...

  19. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...Create a monoclonal Cas9-expression cell line. Especially if you’re doing a screen. I made a few monoclonal..., so some of the cells will still appear green despite having active Cas9 (see tip 2). Pro-Tips 1. To ...about 1 month, which I’d say is pretty amazing. Despite the shifting landscape of research opportunities...

  20. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...data can be acceptable. However, it is your responsibility to determine what counts as adequate validation...the liver (A). In general, RNA levels tend to correspond to protein levels, so you perform immunohistochemistry...to be present and recognizable by your antibody despite the manipulation. Additionally, not all sample ...
Showing: 121 - 140 of 553 results