...it is essential to delve deeply into your chosen field, don't shy away from looking beyond its confines...exposes you to discussions beyond your immediate field, fostering a diverse range of perspectives and sparking...approaches Engaging in mindful meditation, taking field trips, and spending time outdoors in nature or visiting...

...expressed from a separate plasmid. This system amplifies the intensity of the fluorescent signal and enables...scaffold. To increase expression, Tanenbaum et al. modified the GCN4 sequence to increase its alpha-helical...chance of phototoxicity Reduced photobleaching Simplified, long-term single-molecule tracking Caveats ...

...Imperial College London for students in this emerging field. It has gained over 170 members in its first year...Synthetic Biology and exciting new technologies in the field. One of the main aims of the society is to bridge...hopes to help young scientists interested in this field excel and develop their career skills for positions...

...by three of the biggest names in the CRISPR-Cas9 field – Jennifer Doudna, Feng Zhang, and Keith Joung. ...community has clearly made a huge impact on this field. Advances in genome engineering & CRISPR technology... cutting edge science in increasingly important field.  Interested in Addgene's Keystone Poster?  We'll...

...brought out in a Kavli Foundation Round Table. In any field, good communication skills help when it comes to...everyone – not just to others working in your own field. Alternative careers are now the norm for those ...you are ready, tell your friends or others in the field where to find it. A good way to reach others in ...

...adeno-associated viral vector (AAV) samples and created a simplified process, termed viral genome sequencing (VGS)...viral genome sequencing workflow. Using this simplified approach, we sequenced over 100 AAV vector preparations...routinely producing AAV. We believe that this simplified sequencing and analysis approach can be easily...

... For an estimate on availability date or to be notified when a particular catalog item is available for... the vector name and serotype you’d like to be notified about. Controls Plasmid Serotype Name 114472...pAAV-hSyn-DIO-mCherry 59462  AAV2  pAAV-CAG-tdTomato (codon diversified) 37825  AAV8*, AAV9*  pAAV-CAG-GFP 50465 ...

...CRISPR-Cas9 gene editing, transformation may be identified by resistance to an antibiotic. Successful editing... of RUBY highlight its use in crop plants under field conditions or in sterile conditions where disturbing...therefore, are useful for research, and they are identified by a phenotype caused by the segregating mutation...

...strategy to determine the binding kinetics of a purified protein involves immobilization on a solid support...One possibility is covalent modification of the purified target protein with the vitamin biotin. The interaction...apparent affinity for the ligand, especially if the modified amines are located near the ligand binding site...

...has over 65,000 plasmids in the repository, each verified by sequencing, which makes the repository a convenient...simulations and machine learning, they created a classifier that predicts whether a gene was natural or synthetic...synthetic sequences. The researchers then used their classifier to identify synthetic and natural sequences in...

...quicker than larger fragments. These fragments are quantified over time using a fluorescent dye that intercalates... into the DNA such that smaller fragments are quantified first before larger fragments. Figure 1...conditions and forms a blue complex that can be quantified at 595 nm. This method is time consuming and ...

...that interact with the virus. As a result, we identified 332 human proteins that the virus interacts with... by a fairly strong EF1alpha promoter. We have verified expression of correctly-sized proteins by Western...alerts on the Krogan lab's Addgene page to get notified!) Attacking coronavirus, one FedEx package at ...

.... They also come from many different scientific fields, from social sciences to biology, mathematics and... to our volunteer list. We then collected and classified their responses, and used that information to...She is currently based in Spain and works in the field of genetics and cancer. You can follow her on Twitter...

...Editor Textile Technologist/Designer     Fieldwork and traveling Many people are attracted to science...time outdoors and with nature. Experience in the field or in a position that requires travel can show that...into a career, do your research Once you have identified possible careers, the next step is to research...

...fluorescent reporter. Lastly, once a mutant is identified, the PB transposase can easily and seamlessly...overcome these issues the Denham lab developed a modified monomeric red fluorescent protein (RFP), named...Chen M, Kjems J, Febbraro F, Denham M (2018) A Modified Monomeric Red Fluorescent Protein Reporter for...

...fully elucidated. Ron Weiss’s lab at MIT pushed the field further with the creation of the largest miRNA sensor...single-input reporters into multi-input cell classifiers in one assembly step. Find the miRNA reporter... analysis of immune cells has been a gap in the field. Arlene Sharpe’s group at the Harvard Medical School...

...is a certified public accountant (CPA), certified information systems auditor (CISA), and certified health...

...Cas13d proteins they identified have little sequence similarity to previously identified Cas13a-c orthologs...its low immunogenicity. But these studies also identified other advantages, including Cas13d-specific regulatory...

...how they felt when they read papers outside their field, the answers were primarily negative. Stupid, overwhelmed...highlight the technical details of collecting the purified AAV–that the interfaces between layers are blurred...virions are captured at the 40% layer. To collect purified AAV, insert a syringe at the interface of the ...

...targeting. Fast forward a decade, and the CRISPR field has exploded with dozens of Cas enzymes and variants...of the enzymes documented that should narrow the field for a candidate Cas for your experiment. Target...search option. For the former, you simply type in identifier keywords (Cas name, RefSeq ID, etc.) and you ...