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We narrowed to 361 results for: HRE

Showing: 121 - 140 of 361 results
  1. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ... vectors expressing wt humanized Cas9 and up to three gRNAs. Entry vectors containing the U6 promoter ...fragments are PCR amplified for cloning into one of three Zeamays codon-optimized Cas9-containing binary vectors...
  2. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ... editing work? Base transition editing requires three elements. They are, broadly: A Cas protein (catalytically...Cas9n-UGI linkers to improve product purity, and these three improvements represent the fourth generation of ...
  3. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...fluorescence, brightness, and proper maturation. Three new RFPs were isolated and characterized: mScarlet...lifetime of 1.3 ns, quantum yield of 0.20). All three mScarlet variants have shown great performance in...
  4. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...Drosophila cells, targets 13,501 fly genes with at least three independent gRNAs, each chosen strategically to ...79748) enhances bacterial expression of Serine/Threonine kinases while YopH (residues 164-468, Plasmid ...
  5. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    Published
    Nov. 26, 2024, 2:15 p.m.
    ...Heisig, who shared his flow cytometry expertise in three posts. An Introduction to Gating in Flow Cytometry...
  6. Deck the Lab 2022!

    Type
    Blog Post
    Published
    Jan. 17, 2023, 2:15 p.m.
    ...Addgenies will vote on the entries, select up to three winners, and reveal the winning entries on Twitter...
  7. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ... Post: When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes...
  8. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    Published
    Jan. 14, 2021, 1:30 p.m.
    ...one for home. The Addgenies voted, and selected three winners in each category. Congratulations to the...
  9. Addgene's Website Update

    Type
    Blog Post
    Published
    April 8, 2024, 2:15 p.m.
    ...educational resources page, and previews for our three most recent blog posts. Finally, you’ll find everyone...
  10. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ... presented the first detailed picture of Cas9's three-dimensional shape via x-ray crystallography, an ...
  11. Science Communication for Everyone

    Type
    Blog Post
    Published
    April 16, 2019, 1:05 p.m.
    ...changed” to scientists. Keep your message to the main three points of your story. Interested in learning more...
  12. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ...screening approach, the Jennifer Doudna lab identified three different Cas12a inhibitors. Some of these anti-...
Showing: 121 - 140 of 361 results