CRISPR 101: Multiplex Expression of gRNAs
Type
Blog Post
Published
Sept. 10, 2020, 11:45 a.m.
... vectors expressing wt humanized Cas9 and up to three gRNAs. Entry vectors containing the U6 promoter ...fragments are PCR amplified for cloning into one of three Zeamays codon-optimized Cas9-containing binary vectors...