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Showing: 121 - 140 of 327 results

  1. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    Published
    Jan. 21, 2015, 1:30 p.m.
    ... personality types is beyond the scope of this article, there are a few principles that will help you ...Management for Scientists" series More science career articles on our blog Read Joanne Kamen's Post on What ...

  2. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    .... Update (August 3rd, 2017) THE ORIGINAL NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN RETRACTED AND FOLLOW...complex in the cell. As proposed in the original article, to achieve greater efficacy, researchers will ...

  3. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ...research? Then we could get everyone to share their articles on social media and have their friends vote for...to focus on what the experience taught me. The article submitted was hilarious, full of interesting information...

  4. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...sequence all the DNA packaged inside the viral particles (Figure 1). Briefly, packaged DNA is extracted...recombinase-independent recombination occurs in 0.1-0.6% of AAV particles in a given viral prep. With this in mind, we recommend...

  5. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...methodologies have been extensively described in other articles; so if you are interested in learning more about...Plasmids 101 blog posts New to plasmids? Read our article on What is a Plasmid? Download the Plasmids 101...

  6. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ...provide the necessary components to make lentiviral particles. Stable, tunable expression of Cas9 and/or gRNA... AAV transfer vector and used to generate AAV particles. Transient or stable expression of SaCas9 and/...

  7. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...cytosol, which is slightly acidic, or synaptic vesicles). Some FPs have different ex/em spectra (e.g. ... Green Fluorescent Protein (GFP) Three Review Articles on the Different Types of FPs: Stepanenko et....

  9. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...cells released transgene-containing lentiviral particles into the media, which could be collected for experimental...Addgene’s most requested plasmids! Lentiviral particles with the VSV-G envelope have high infectivity ...

  10. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ...transferable skills? PLoS One recently published an article identifying gaps in scientists' transferrable skills...skills in that big job interview, check out this article from The Muse. Kai Strickland is a former Customer...

  11. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...including the E1 region). The space in the viral particle’s capacity that is made available by this deletion... et al., 1999).  The ability of the adenovirus particle to activate the immune system, and of the infection...

  12. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...Roberts, Prasad S. Sarangapani, Protein aggregation, particle formation, characterization & rheology, Current...vials causes cavitation, protein aggregation, and particle formation. J Pharm Sci. 2015 Feb;104(2):602-11...

  13. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...

  15. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...this way data is moved from a static and isolated vehicle and becomes part of the dynamic and connected record...repositories have been launched and are now a main vehicle for making research data available for public access...

  16. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ... resources on the Addgene blog: Browse CRISPR articles from our CRISPR topic overview Find other CRISPR...CRISPR methods for bacteria Find all articles about microbiology Resources on Addgene.org: Visit the CRISPR...

  18. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...cell will be transduced by no more than one viral particle, giving a low multiplicity of infection (MOI)....chances of a cell being infected by only one viral particle, but it also increases the chances that a cell...

  19. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...recent study investigated the injection of AAV8 particles expressing an activating chemogentic receptor ...activate the Drd1 neurons, they injected AAV2 particles into a Drd1-Cre transgenic mouse to deliver Cre-dependent...

  20. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ... that compose the secretory pathway, endocytic vesicles, mitochondria, lysosomes or they are secreted ...11. Jokitalo, Eija, et al. "Golgi clusters and vesicles mediate mitotic inheritance independently of the...
Showing: 121 - 140 of 327 results