We narrowed to 332 results for: ICL
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TypeBlog PostPublishedOct. 13, 2020, 1:15 p.m....the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...
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7 Simple Strategies to Resolve Conflicts with Difficult Supervisors
TypeBlog PostPublishedJan. 21, 2015, 1:30 p.m.... personality types is beyond the scope of this article, there are a few principles that will help you ...Management for Scientists" series More science career articles on our blog Read Joanne Kamen's Post on What ... -
Hot Plasmids: Summer 2025
TypeBlog PostPublishedAug. 28, 2025, 1:15 p.m....more in the repository through our Hot Plasmids articles. Here's what you'll find in this post: Bright..., et al., 2025). AF was fused to a presynaptic vesicle protein (VAMP2) and an epitope tag (ALFA-tag, or... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog PostPublishedAug. 4, 2016, 2:30 p.m..... Update (August 3rd, 2017) THE ORIGINAL NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN RETRACTED AND FOLLOW...complex in the cell. As proposed in the original article, to achieve greater efficacy, researchers will ... -
AAV Vector Quality Control: Going the Extra Mile with NGS
TypeBlog PostPublishedSept. 12, 2017, 1:44 p.m....sequence all the DNA packaged inside the viral particles (Figure 1). Briefly, packaged DNA is extracted...recombinase-independent recombination occurs in 0.1-0.6% of AAV particles in a given viral prep. With this in mind, we recommend... -
Plasmids 101: 5 factors to help you choose the right cloning method
TypeBlog PostPublishedAug. 21, 2018, 12:31 p.m....methodologies have been extensively described in other articles; so if you are interested in learning more about...Plasmids 101 blog posts New to plasmids? Read our article on What is a Plasmid? Download the Plasmids 101... -
Experimenting with New Careers while in Grad School
TypeBlog PostPublishedFeb. 25, 2016, 3:30 p.m....research? Then we could get everyone to share their articles on social media and have their friends vote for...to focus on what the experience taught me. The article submitted was hilarious, full of interesting information... -
CRISPR 101: Mammalian Expression Systems and Delivery Methods
TypeBlog PostPublishedSept. 24, 2015, 2:30 p.m....provide the necessary components to make lentiviral particles. Stable, tunable expression of Cas9 and/or gRNA... AAV transfer vector and used to generate AAV particles. Transient or stable expression of SaCas9 and/... -
Which Fluorescent Protein Should I Use?
TypeBlog PostPublishedMay 20, 2014, 2:06 p.m....cytosol, which is slightly acidic, or synaptic vesicles). Some FPs have different ex/em spectra (e.g. ... Green Fluorescent Protein (GFP) Three Review Articles on the Different Types of FPs: Stepanenko et.... -
Transferable Skills Guide: Identifying Your Transferable Skills
TypeBlog PostPublishedJan. 11, 2018, 3:29 p.m....transferable skills? PLoS One recently published an article identifying gaps in scientists' transferrable skills...skills in that big job interview, check out this article from The Muse. Kai Strickland is a former Customer... -
An Introduction to Adenovirus
TypeBlog PostPublishedOct. 3, 2023, 1:15 p.m....including the E1 region). The space in the viral particle’s capacity that is made available by this deletion... et al., 1999). The ability of the adenovirus particle to activate the immune system, and of the infection... -
Antibodies 101: Producing Recombinant Antibodies
TypeBlog PostPublishedAug. 13, 2024, 1:15 p.m....Roberts, Prasad S. Sarangapani, Protein aggregation, particle formation, characterization & rheology, Current...vials causes cavitation, protein aggregation, and particle formation. J Pharm Sci. 2015 Feb;104(2):602-11... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog PostPublishedDec. 3, 2019, 11:38 a.m....search tool lets you access plasmids from journal articles. From paper to plasmid Authors regularly indicate...also find the associated plasmids through the article’s PubMed ID (PMID) ...or DOI Or get some keywords... -
Lentiviral Vector Uses and Overview
TypeBlog PostPublishedMay 19, 2016, 2:30 p.m....cells released transgene-containing lentiviral particles into the media, which could be collected for experimental...Addgene’s most requested plasmids! Lentiviral particles with the VSV-G envelope have high infectivity ... -
Plasmids 101: Shuttle Vectors
TypeBlog PostPublishedMay 20, 2025, 1:15 p.m....machinery to manufacture infectious viral particles. Those particles are collected to deliver the gene to ... -
Analyzing CRISPR Editing Results with ICE from Synthego
TypeBlog PostPublishedMay 8, 2018, 1 p.m....This article was contributed by Jessica Roginsky, Scientific Support Lead at Synthego. Article source... -
Viral Vectors 101: Preparing Pooled Libraries
TypeBlog PostPublishedJuly 13, 2023, 1:15 p.m....cell will be transduced by no more than one viral particle, giving a low multiplicity of infection (MOI)....chances of a cell being infected by only one viral particle, but it also increases the chances that a cell... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog PostPublishedSept. 17, 2019, 12:40 p.m....recent study investigated the injection of AAV8 particles expressing an activating chemogentic receptor ...activate the Drd1 neurons, they injected AAV2 particles into a Drd1-Cre transgenic mouse to deliver Cre-dependent... -
CRISPR-based Genome Editing Systems For Editing in E. coli
TypeBlog PostPublishedApril 13, 2021, 1:15 p.m.... resources on the Addgene blog: Browse CRISPR articles from our CRISPR topic overview Find other CRISPR...CRISPR methods for bacteria Find all articles about microbiology Resources on Addgene.org: Visit the CRISPR... -
Hot Plasmids - August 2020
TypeBlog PostPublishedAug. 18, 2020, 1:15 p.m....the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...