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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 316 results for: Mut

Showing: 121 - 140 of 316 results

  1. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    Published
    Dec. 10, 2020, 1 p.m.
    ...tissues with Cas9 and a gRNA designed to target a mutated gene. In a Scientific Reports paper introducing...-transformed cells yielded rates of targeted mutagenesis similar to those achieved by isogenic AdVs encoding...CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array of human cells. Sci Rep 4:...

  2. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...this process accounts for a percentage of the mutations seen with TALENs and CRISPR. MMEJ is active during...and Ling Lu. (2016). “Highly efficient CRISPR mutagenesis by microhomology-mediated end joining in Aspergillus...Other Genome Engineering Tools like Sleeping Beauty Mutase Find the Ideal CRISPR Software for Your Experiment...

  3. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...TBD domains, part of the HB domain, and several mutations to improve solubility and stability (Figure 2)...in natural DNA repair pathways and spontaneous mutations.  Here, the researchers were able to target multiple...abscessus either simultaneously or stepwise, achieving mutation efficiencies of up to about 9% of colonies (102...

  4. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...transfer’. Resistance can also arise from random mutations in the genetic sequence that happen to be beneficial... if some didn’t develop, either through random mutation or horizontal gene transfer, means to defend themselves...effective drug targets (ones that are less likely to mutate or change in order for the bacterium to survive...

  5. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...one of the FLP-FRT systems in your experiments. Mutated FRT and loxP sites in Flex Vectors – inversion ...common means to achieve ligand control is to fuse a mutant estrogen receptor (ER) ligand binding domain (LDB...Properties of FLP Recombinase Evolved by Cycling Mutagenesis.” Nature biotechnology 16(7): 657–62. PubMed ...

  7. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...fluorescent tag or the introduction of a specific mutation, one generally relies on homology directed repair...experimental conditions used, led to no detectable mutations (Veres et al., 2014). When working with single-cell...Musunuru K (2014) Low Incidence of Off-Target Mutations in Individual CRISPR-Cas9 and TALEN Targeted Human...

  8. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...Electrochemiluminescent assay for detection of extremely rare mutations based on ligase reaction and bead enrichment."...direct identification of allelic composition of mutated DNA in a mixed population of excess wild‐type DNA...probe for the detection of low-abundance point mutations." Analytical chemistry 86.1 (2013): 900-906. PubMed...

  9. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...vasculature to feed themselves, use the blood stream to commute to and invade multiple organs, and most importantly..., tumor penetration, inadequate concentration, mutation of cell surface targets by tumor cells, are a ...the most important: How will cancers evolve and mutate based on the immunotherapeutics we use on them?...

  11. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...being able to specify and correct single-base mutations without introducing DSBs at all. Base editing ...properties of the individual Cas9. They also mutagenized the cytidine deaminase portion of the base editor...are smaller and less likely to cause off-target mutations than those derived from naturally occurring cytidine...

  12. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ... V2.0 from it's predecessor, which had a point mutation in the puromycin resistance gene that made it ...less effective in certain cell lines. This point mutation has been corrected in V2.0. pSpCas9(BB)-2A-Puro...

  14. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ...disease typically caused by a single R206H point mutation on the receptor ACVR1. ACVR1 triggers appropriate...regeneration in healthy patients. However, the R206H mutation makes ACVR1 responsive to members of the ubiquitous...

  15. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ...repaired by base excision repair, but can lead to mutations in single stranded DNA undergoing replication....endA negative, meaning the gene has been removed/mutated to neutralize the nuclease activity. For strains...

  16. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...inactivates the gene and acts as a marker for the mutation. When designing these types of experiments it ...not to use the same resistance cassette for the mutation and for plasmid selection. Additionally, scientists...

  17. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ... Blog Perform Site Directed Mutagenesis by PCR Use REPLACR Mutatgenesis to Easily Generate Insertion ...

  18. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...Other labs have used PCR-based screening to detect mutants, but this method limits screening to a few hundred...other genome modifications, including targeted mutations. SEC-based systems may prove effective in other...

  19. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...toxin, indicating a 70% frequency of biallelic mutations. Importantly, hESCs retained their stemness, indicating... remove the risk of viral integration causing mutations and gene expression changes elsewhere in the genome...

  20. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...the preferred pathway for generating knock-in mutations, and NHEJ is an error-prone pathway frequently...frequently introducing frameshift knockout mutations. Thus, if genetic manipulation one way or the other (knock-in...
Showing: 121 - 140 of 316 results