Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 362 results for: Numb

Showing: 121 - 140 of 362 results

  1. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...Deciding which type of injection to use depends on a number of factors. You have to weigh the pros and cons...what’s being injected, the type of experiment, the number of mice, the potential pain or discomfort of the...

  2. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ...including gene expression profiling, studying copy number variation, and molecular diagnostics.  Digital ...concentration Gene expression profiling Copy number variations Quantify RNA levels Molecular Diagnostics...

  4. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...applications require teaching experience or high numbers of publications, which may take a longer time to...billion dollars and at least a decade of work. The number of steps, decisions and people involved is staggering...

  6. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ... need. Unlike lentivirus, AAV requires a higher number of viral particles for efficient gene transfer....qPCR Overview: AAV titering by qPCR quantifies the number of genome-containing viral particles that are present...

  7. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...is contaminated? No - what really matters is the number of hits to the same sequence (gene). In a clean... this isn’t cause for concern. However, a large number of hits to one sequence/gene - we set our threshold...

  8. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...that you’ve printed your poster and practiced a number of times, it’s time to expertly show off your science...thirty-minute-long presentations, which constrains the number of posters you can see. If you are pressed for ...

  9. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...and how the culture of the university impacts the number of women reaching senior positions. There has been...for that too. I’m more in touch with a remarkable number of female scientists whose tweets I find really...

  10. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...Interestingly, he also adds to the disproportionately large number of honorees to have trained under a previous laureate...undergraduate education in the sciences by making a greater number of students active participants in the research...

  11. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...Kim and Morrison method, you can just adjust the number of glass filters you use depending on the size ... DNA purification kit, you can still reduce the number of spin columns you need to complete your research...

  12. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...inflorescences begin to grow. This will also increase the number of inflorescences overall, which may improve transformation...transgene. You can optionally remove them to reduce the number of non-transformed seeds from the pool you will...

  13. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...Rev and one encoding Gag and Pol, increasing the number of recombination events necessary to create a replication-competent...lead to lower viral titers due to higher plasmid number. For more information on the key differences between...

  14. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...-10 colonies in total to test, depending on the number of background colonies on your no ligation control... insert DNA. Colony PCR will greatly reduce the number of clones you’ll need to send for sequencing, but...

  15. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...If you feel like you may have low participation numbers for an event you are planning, dangle the carrot...  4. Utilize your office space too Balance point number 3 with doing fun things within your office. This...

  16. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...drugs against intractable diseases. Currently, a number of labs are using enChIP, and I'm very happy to...very difficult for us to respond to such a large number of requests by ourselves without Addgene. So, when...

  17. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...disciplines and scientists employ GFP for a wide number of functions, including: tagging genes for elucidating...general epitope tag for protein purification and a number of commercial antibodies to GFP are available. ...

  18. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...and state that it would help you manage a large number of files as part of the job. Structure of a cover...adjective that describes you] scientist with over [number] years of experience in [field of research], and...

  19. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...arabinose-inducible promoter pBAD and a low-copy number origin of replication (p15A) worked best under ...Stabilizing the gvpA transcript with WPRE reduced the number of copies of gvpA needed to increase expression...

  20. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ... be unstable and lack precise control over copy number. MUCICAT system contains 4 plasmids: pDonor, pTnsABC...transposition-inducing agar lead to higher cargo copy numbers. (D) The effect of gene dosage on N-acetylglucosamine...
Showing: 121 - 140 of 362 results