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We narrowed to 548 results for: PPL

Showing: 121 - 140 of 548 results

  1. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...plasmid. Although only wt hCas9 entry vectors are supplied with the kit, you can clone your own entry vectors...vector does not contain Cas9, so you’ll need to supply it with another plasmid.   Figure 3: Comparison...genomic modification. In this system, Cas9 must be supplied on a separate plasmid. Multiplexing in Drosophila...from two Drosophila U6 promoters. Cas9 must be supplied on a separate plasmid. Multiplexing in E. coli...

  2. Plasmid Grant to Aid Cervical Cancer Screening in Ghana

    Type
    Blog Post
    Published
    Nov. 8, 2016, 3:30 p.m.
    ...expected when we originally sent out the call for applications. Cervical cancer screening in Ghana Gladys is...Papillomavirus (HPV). As Gladys wrote in her application, “Cervical Cancer is the leading cause of cancer...Seeding Labs Plasmid Grant to a third round of applicants at the start of 2017 and would like to thank ...

  3. Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs

    Type
    Blog Post
    Published
    June 15, 2016, 1:30 p.m.
    ...concluded, Addgene and Seeding Labs will be opening applications for a second round of plasmid awards on June...excited to discover what new and creative ideas applicants will propose for plasmids from the Addgene community...regions in the near future. Additional Resources Apply to Recieve Equipment through Seeding Labs' Instrumental...

  4. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...that the antigen being detected in the intended application — here, flow cytometry — is the desired target...antibodies. This is the most usual level of evidence supplied to support the suitability of an antibody for ...quantification of antibody performance in major research applications. eLife, 12, RP91645. https://doi.org/10.7554...of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72. eLife...

  5. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    Published
    Sept. 29, 2015, 2:30 p.m.
    ...for both transient and stable gene expression applications in plants. This smaller, easily customizable...these properties of pSiM24 are suitable for many applications, the plasmid can also be customized due to its...only for plant engineering and biotechnology applications, but also for foundational studies of promoter...

  6. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...Early CRISPR applications were often limited by the low editing efficiency of homology-directed repair...making them a promising tool for cell therapy applications. Dual base editors What about combining the ...editors with increased activity and therapeutic application. Nature Biotechnology, 38(7), 892–900. https:.... (2017). Improving the DNA specificity and applicability of base editing through protein engineering ...

  7. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    Published
    Jan. 21, 2020, 2:30 p.m.
    ...results section, but you had to dig through the supplemental files to see the actual data. Now you’re wondering...datasets, which are often obscured in large supplementary data files, BioGRID ORCS makes it possible for...sgRNA-level sequence read scores. The original supplemental data tables available with the publication are...

  8. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...a custom, high-throughput screening platform to apply saturating mutagenesis on 21 positions in the protein...blot, but we are actively testing it in other applications! If you try it out in a new assay, let us know...GFP-smFP-myc expressed from Plasmid 98926. See the Applications section on the Anti-c-Myc [9E10] page for more...

  10. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...aggregation, it’s recommended for most imaging applications. What can you do with SunTag? Tanenbaum et al...flames,” it’s clear that the system is broadly applicable across many areas of research. Beyond fluorescence...activation of target genes, but we’ll save that application for another day! References 1. Tanenbaum, Marvin...

  11. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...to sdAbs becoming valuable tools for diverse applications. For example, RANbodies can be used as an alternative... excitement around using sdAbs in diagnostic applications. For example, fluorescently and radioactively...inspired this versatile family of tools, the applications they have already enabled, and the discoveries...

  12. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...various species and describe an assortment of applications. Despite the ubiquity of CRISPR/Cas toolkit,... multiple plasmids used for various platform applications. For more information on CRISPR/Cas, check out...for your research. Do you have a cool idea or application that involves CRISPR/Cas technology? Let us know...

  13. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...community about how the antibodies work in various applications, enabling you to better plan your experiments...the target antigen, and performance in various applications are included in the full webpage. Educational...recombinant antibody production as well as antibody applications. In addition, basic and advanced affinity reagents...

  14. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ... be used as a fusion tag for a wide range of applications from traditional imaging to FRET and stochastic...fluorescent protein: structural characterization and applications in fluorescence imaging." Biochemical Journal...identification of the molecular determinants of color and applications in fluorescence imaging." BMC biology 6.1 (2008...

  15. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...protein-only transduction method and its potential applications to CRISPR/Cas9 genome editing.   Protein-only...transducing primary cells. What if, instead of supplying a DNA construct, they could simply induce cells...Diego D'Astolfo. CRISPR/Cas9: a potential application of iTOP Having tested iTOP using Cre-lox recombination...

  16. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ...on the marketing or business background of the applicant. However, although this position did look for ...experience or a marketing interest that would be able to apply themselves immediately to the position. In case ...understand enough to relate it to the position I was applying for. After all I was only going to be interviewed...

  17. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...packaged into viral vectors for potential in vivo applications or multiplex CRISPR screening.   Using tRNAs...and efficient in plasmid and viral vectors. In applications when repetitive tRNA sequences must be avoided...construct DAP array for multiplex gene editing applications, we provide detailed manual and online design...

  18. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ... 3 Applied and Environmental Microbiology  1.6%  1.2% 0.75   2.3 4 2010 167  Applied and Environmental...Genetics & Heredity, Cell Biology, Biotechnology & Applied Microbiology, Biochemistry & Molecular Biology,...

  19. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...iontophoresis (applying small electric currents via inserted electrodes) may be applied, but these methods...intracellularly spread away from the site of application and aid in visualization of the morphology of...into muscle) as opposed to direct intracerebral application, which is more invasive and has a much higher...

  20. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...advantage of the extensive supply of transgenic reporter lines that supply a variety of GFP expression...Fishell   Optogenetics Optogenetics continues to supply neuroscience with ever more efficient tools for...
Showing: 121 - 140 of 548 results