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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 604 results for: REV

Showing: 121 - 140 of 604 results

  1. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...treatment regimen and were not preexisting. This was previously debated in the cancer field and was difficult...be amplified using the same sets of forward and reverse primers). The abundance of a particular barcode...replicate; prior to treatment, there are small (previously undetectable) populations of cancer cells that...that combination treatment with multiple drugs reveals even smaller preexisting populations with resistance...

  2. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...In previous posts for our Plasmids 101 series, we examined a number of important plasmid elements – promoters...and the region of DNA between the loxP sites is reversed.   Deletion: If the sites face in the same direction... cassette) upstream of a gene of interest will prevent gene expression in the absence of Cre. In the presence...recombination event in a single system. The Saccharomyces cerevisiae FLP-FRT recombination system is another site-...

  3. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...fluorogen A split fluorescent reporter with rapid and reversible complementation. Tebo AG, Gautier A. Nat Commun...options. While the BiFC interaction is typically irreversible and requires some time for chromophore maturation...maturation, splitFAST displays rapid and reversible complementation. TagBiFC (split HaloTag) provides maximum...important for their interaction. You can read our previous blog post for more on BiFC principles and experimental...

  4. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...low cost and quick point-of-care diagnosis kits (reviewed in Tsang and LaManna, 2020). To see even more ...uses loop-mediated isothermal amplification after reverse transcription (RT-LAMP) and a colorimetric readout...completed in just 45 minutes. The team deposited the reverse transcriptase, protease, and Bst polymerase constructs... this toolkit include ones for expressing MMLV reverse transcriptase and Taq polymerase that can be used...

  5. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ..., you will need to correct it). It’s easy for reviewers to spot when an application is rushed (whether...that you ask a PI or mentor (your supervisor/ a previous supervisor / someone else in the department) to... times and will have useful advice about what reviewers in your area are looking for. Remember though,...for grant success The two most important things reviewers will be looking for in your research proposal ...

  6. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    Published
    July 2, 2020, 12:40 p.m.
    ...broken during cross-link reversal (not shown in diagram). Cross-links are reversed so that DNA fragments ...cells (~5x107). Together, these two requirements prevent the use of CAPTURE with primary cells or rare cell...multiplexed CAPTURE 2.0 largely replicated interactions previously identified with the original CAPTURE method. ...

  7. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ...then dropped to a range in which the forward and reverse primers are stable. At this temperature the primers...can also be used to quantitate RNA levels using reverse transcription-qPCR (RT-qPCR). The first step of...process requires RNA to be converted to cDNA using reverse transcription and the cDNA is subsequently quantified...template is amplified using several forward and reverse primer sets.  Multiple template PCR - multiple...

  8. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ...new managers and I had a “Eureka” moment while reviewing the results of my Myers-Briggs test:  my undergrads...motivation, but he didn’t want to spend hours reviewing papers to piece together the best protocol;  he...associate feedback with end-of-the-year performance review, but feedback should be given throughout the year...References Becoming the boss. Harvard Business Review, January 2007. Three Rules for Managing a Successful...

  9. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ... are voltmeters for cells! In this blog we will review how these indicators work, their general applications...of the membrane, a concern with several of the previously described systems. Several adaptations of this...   Viral Vectors and Voltage Indicators As previously mentioned, cellular voltage is measured at the... Components of the types of voltage indicators reviewed.   References and Resources   References ...

  10. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...Cas13-based RNA editing system would likely be reversible and would avoid genomic off-targets or indels... RNA knockdown. In initial tests, Cas13b from Prevotella sep. P5-125 (PspCas13b) yielded 62.9% average...editing plasmids here! Future directions Building on previous RNA targeting work with Cas13, REPAIR is the first...edit RNA has multiple advantages, including reversibility and use in non-dividing cells. With the expanded...

  11. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...ambitious, that’s because it is. Zimmer, whose previous books focused primarily on fluorescent proteins...racism and sexism in science, problems with peer-reviewed publications and funding, bioethics in the age...unlikely to be surprised by many of Zimmer’s revelations. The issues he discusses have been making the...of his book today--the day you are reading this review--in the current period of rapid flux, the chapter...

  12. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ... functional copy of your plasmid of interest, preventing this issue. The genes and their antibiotics   ...produces beta-lactamase to degrade amp and therefore prevent cell death. One of the cool things about amp is...susceptible to degradation in agar plates. This prevents satellite colonies from forming while effectively...expressing a protein that directly binds to zeocin, preventing it from binding DNA. Zeocin is an all-in-one ...

  13. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...within a CRISPR guide can prevent the guide from binding to the genome or prevent Cas9 from cutting, both...genome could affect their analysis. Their work revealed that cell lines from individuals of African descent...Perturbation Platform to modify the CRISpick algorithm to prevent guides from being designed that target regions ...

  14. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...a different mechanism - the PhoCl protein is irreversibly cleaved when exposed to light. Robert Campbell...marker-free coselection, analogous to methods previously used in C. elegans, targets the sodium/potassium... the point mutations created by Agudelo et al. prevent ouabain binding to ATP1A1 but do not compromise...for TZAP, a Kruppel-like zinc finger protein (previously named ZBTB48) in regulating the upper-bound of...engineering that enables new applications, such as reversing the effects of dCas9 binding to a genomic locus...

  15. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...there are several parallel mechanisms in place (for review see Maday et al., 2014). It is currently unclear...neurotoxicity, and is lethal if left untreated (for review see Dietzschold et al., 2008). Herpes Simplex Virus...neuronal connection via a single synaptic step, thus preventing further widespread expansion of RABV. This improvement...combination with conditional transgenic mice, to reveal not only the anatomy of neuronal connections, but...Cell type-specific genetic and optogenetic tools reveal novel hippocampal CA2 circuits. Nat Neurosci 17...

  16. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...we give people the opportunity to speak out and prevent groupthink? Here are some ideas that I have found...a member of our Business Development team would review it. This survey generated 26 ideas, over half of...mistakes that kill your productivity. Harvard Business Review. Retrieved January 14, 2022, from https://hbr.org... spur innovation on your team. Harvard Business Review. Retrieved January 14, 2022, from https://hbr.org...

  17. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...participant in the training. In this blog we will review the preparation and planning you can do to build...different way of doing something then you’ve done previously) you can bring those questions to your training...questions throughout training, while others prefer to review their notes, process what they’ve just learned,...Show up prepared and take training seriously, as reviewed above. Offer to help clean up and/or make necessary...

  18. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ... the Wisconsin research community were to: (1) prevent vendor lock in by making it easy to get data out... to a selected Slack channel for discussion and review. It’s also possible to capture Slack conversations... and/or import and convert it into RSpace. The reverse flow is also supported, so it’s possible to re-...making research data available for public access, review, and query. Gates Open Research and Wellcome Open...

  19. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ... limited to 0-5%. This blog post covers a brief review of different basic BE technologies and their adaptation...making an original C-G pair converted to T-A or reverted to the original C-G by base excision repair mediated... by introducing a point mutation (H193Y). The reversion of this mutation by an adenine base editor would...in rice. In Genome Engineering: The CRISPR-Cas Revolution, Cold Spring Harbor Laboratory (2018). Additional...

  20. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...Previously, we provided a general overview of the QC process at Addgene. All plasmids go through this...the expected sequence, the QC team can manually revisit the assembly in a program called Geneious. Within...the assembly. Geneious will show how reads align, reveal multiple contigs, and highlight sequence identities...mismatch or indel flags the sequence for manual review by one of our QC team members. This process saves...
Showing: 121 - 140 of 604 results