Skip to main content

We narrowed to 481 results for: SEC

Showing: 121 - 140 of 481 results
  1. CRISPR Guide Update

    Type
    Blog Post
    Published
    Oct. 10, 2024, 1:15 p.m.
    ... disposal. With this update, we have added new sections to share and describe some incredible new CRISPR...Highlights include a much-anticipated prime editing section and additions describing CRISPR’s ability to make...
  2. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ... primary antibody overnight at 4 °C and their secondary antibody for 1–2 hrs at room temperature, which...providing flexibility in assay design. Conjugated secondary antibodies are relatively cheap and easy to source...: Selecting the Right AntibodyAntibodies 101: Secondary Antibodies Resources on Addgene.org Addgene's ...
  3. 2021 Deck the Lab Winners!

    Type
    Blog Post
    Published
    Jan. 25, 2022, 2:15 p.m.
    ... any further ado, we present to you: our first, second, and third place winners!  1st Place: Krampus Saves...creativity, also receives an honorable mention for their second submission.    Our Cultured Warrior (made by Nate...
  4. New Educational Resource: The Addgene Videos Page

    Type
    Blog Post
    Published
    June 16, 2017, 2 p.m.
    ...about Addgene. The page is divided into three main sections: Protocols To complement our written protocol... see here? Give us suggestions in the comments section below! Additional Resources on the Addgene Blog...
  5. Science Communication Snapshot: DayCon 2015

    Type
    Blog Post
    Published
    June 17, 2015, 9:04 p.m.
    ... public and help make science more transparent. Second, SITN helps graduate students develop their communication...engage the public - let us know in the comments section! More information about SITN: DayCon event website...
  6. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ...eight modular repeats, each of which binds a consecutive base in its RNA target. By changing amino acids...specificity of other RNA binding domains depends on the secondary structure of RNA. As a result, targeting the RNA...
  7. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    Published
    April 27, 2017, 2:30 p.m.
    ...This is the second half of a two-part interview with Vini Mani and Amy Gilson from Science in the News... get involved in science communication. In this second half of our conversation with Vini Mani and Amy...
  8. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ...subtilis and the yeast Pichia pastoris. The improved secretory capacities of these microbes make them useful ...peptide (SP) that routes the protein through the secretory pathway to improve folding and disulfide bond ...
  9. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ...DNA—to cut, nick, or bind. DNA CRISPR array: The section of the bacterial chromosome that stores a genetic...trans-activating CRISPR RNA” base pairs to the repeat section of the crRNA, forming the repeat:antirepeat duplex...
  10. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...and there are unique benefits to using sdAbs as secondary antibodies. sdAbs, however, really shine in vivo...the Addgene blog Introduction to Antibodies  Secondary Nanobody Toolbox No Llamas Required - Synthetic...
  11. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...missed something hot? You can pitch a Hot Plasmids section (antibodies and viral preps welcome!) here. Here's...expressed from Plasmid 98926. See the Applications section on the Anti-c-Myc [9E10] page for more details....
  12. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    Published
    April 25, 2024, 1:15 p.m.
    ... beauty of serendipity often emerges at the intersections of disciplines. Cast your net wider, explore...Maintaining openness and looseness of spirit The second crucial element in harnessing the power of serendipity...
  13. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ... This response is immediate, on the scale of milliseconds, and can precisely link a neuron's activation...controlled Unique advantages Mapping the brainDissecting circuits Studying GPCR pathways   Acknowledgements...
  14. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...tissue takes a week of multiple people performing dissections. With CUT&RUN, we have had success with significantly... IgG, it is helpful to incubate with a rabbit secondary antibody when using a non-rabbit primary, prior...
  15. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ... family, he did not seem much different than my second son of similar age. When he came to visit us and...with Addgene and join us in the fight against FOP. Second, Addgene will be hosting the AJ’s Boston League...
  16. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...for the transduction step. Viral particles are secreted into the cellular media, making viral collection... of cell or organism context you are targeting? Second, what is the biological outcome you want to achieve...
Showing: 121 - 140 of 481 results