We narrowed to 468 results for: SEC
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TypeBlog PostPublishedJan. 25, 2022, 2:15 p.m.... any further ado, we present to you: our first, second, and third place winners! 1st Place: Krampus Saves...creativity, also receives an honorable mention for their second submission. Our Cultured Warrior (made by Nate...
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New Educational Resource: The Addgene Videos Page
TypeBlog PostPublishedJune 16, 2017, 2 p.m....about Addgene. The page is divided into three main sections: Protocols To complement our written protocol... see here? Give us suggestions in the comments section below! Additional Resources on the Addgene Blog... -
Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)
TypeBlog PostPublishedJuly 26, 2018, 12:46 p.m....with limited spectral overlap are used together. Second, KTRs are easy to design. The key determinants ... activity in specific subcellular compartments. Second, because KTRs shuttle between the nucleus and the... -
Science Communication Snapshot: DayCon 2015
TypeBlog PostPublishedJune 17, 2015, 9:04 p.m.... public and help make science more transparent. Second, SITN helps graduate students develop their communication...engage the public - let us know in the comments section! More information about SITN: DayCon event website... -
Designer PUF Proteins for Any RNA Target
TypeBlog PostPublishedJune 17, 2014, 7:57 p.m....eight modular repeats, each of which binds a consecutive base in its RNA target. By changing amino acids...specificity of other RNA binding domains depends on the secondary structure of RNA. As a result, targeting the RNA... -
Six Spooky Science Stories and Halloween at Addgene
TypeBlog PostPublishedOct. 31, 2019, 1:06 p.m....forces its insect host to dive into water. There, the worm emerges to find a mate, leaving the insect dead ... -
SciComm with the Experts at Science in the News Part 2
TypeBlog PostPublishedApril 27, 2017, 2:30 p.m....This is the second half of a two-part interview with Vini Mani and Amy Gilson from Science in the News... get involved in science communication. In this second half of our conversation with Vini Mani and Amy... -
Easing the Protein Purification Process with pCri
TypeBlog PostPublishedJune 19, 2015, 3:08 p.m....subtilis and the yeast Pichia pastoris. The improved secretory capacities of these microbes make them useful ...peptide (SP) that routes the protein through the secretory pathway to improve folding and disulfide bond ... -
Build Your CRISPR Vocabulary
TypeBlog PostPublishedJuly 2, 2024, 1:15 p.m....DNA—to cut, nick, or bind. DNA CRISPR array: The section of the bacterial chromosome that stores a genetic...trans-activating CRISPR RNA” base pairs to the repeat section of the crRNA, forming the repeat:antirepeat duplex... -
Year of the Camelid: Antibody Style
TypeBlog PostPublishedMay 9, 2024, 1:15 p.m....and there are unique benefits to using sdAbs as secondary antibodies. sdAbs, however, really shine in vivo...the Addgene blog Introduction to Antibodies Secondary Nanobody Toolbox No Llamas Required - Synthetic... -
Embracing Serendipity: A Crucial Element in the PhD Journey
TypeBlog PostPublishedApril 25, 2024, 1:15 p.m.... beauty of serendipity often emerges at the intersections of disciplines. Cast your net wider, explore...Maintaining openness and looseness of spirit The second crucial element in harnessing the power of serendipity... -
Hot Plasmids - October 2022
TypeBlog PostPublishedOct. 6, 2022, 1:15 p.m....missed something hot? You can pitch a Hot Plasmids section (antibodies and viral preps welcome!) here. Here's...expressed from Plasmid 98926. See the Applications section on the Anti-c-Myc [9E10] page for more details.... -
Chemogenetics vs. Optogenetics: Which Method Should I Choose?
TypeBlog PostPublishedJune 11, 2020, 1:34 p.m.... This response is immediate, on the scale of milliseconds, and can precisely link a neuron's activation...controlled Unique advantages Mapping the brainDissecting circuits Studying GPCR pathways Acknowledgements... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog PostPublishedMay 21, 2020, 1:15 p.m....ribozyme. In the second-generation SPARC2 system, HDVR is placed upstream of the second attP site to cleave... -
CUT&RUN: An Improved Method for Studying Protein-DNA Interactions
TypeBlog PostPublishedFeb. 13, 2018, 2:51 p.m....tissue takes a week of multiple people performing dissections. With CUT&RUN, we have had success with significantly... IgG, it is helpful to incubate with a rabbit secondary antibody when using a non-rabbit primary, prior... -
Running for Rare Disease, Running for FOP, Running for AJ
TypeBlog PostPublishedSept. 15, 2016, 2:30 p.m.... family, he did not seem much different than my second son of similar age. When he came to visit us and...with Addgene and join us in the fight against FOP. Second, Addgene will be hosting the AJ’s Boston League... -
Viral Vectors 101: Transductions
TypeBlog PostPublishedApril 30, 2024, 1:15 p.m....for the transduction step. Viral particles are secreted into the cellular media, making viral collection... of cell or organism context you are targeting? Second, what is the biological outcome you want to achieve... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog PostPublishedSept. 21, 2021, 1:15 p.m....a stronger promoter and different biochemical secondary energy sources to increase protein yields from...some of the new viral preps from recent months: Second generation GRAB dopamine sensors from Yulong Li... -
Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource
TypeBlog PostPublishedMay 25, 2021, 2:29 p.m....antibody-producing cell lines (hybridomas) that secrete antibodies in culture and provide renewable reagents...plasmid-based and offer numerous advantages, including more secure archiving, unambiguous molecular definition, and... -
CRISPR Challenges: Standardization and Homology Directed Repair
TypeBlog PostPublishedFeb. 14, 2018, 2:36 p.m....admit to being a DNA repair newbie - diagrams of resection and strand invasion are like a foreign language... tool or resource? Let us know in the comments section!...