...popular and represent the diversity of tools we share on behalf of scientists. 9. Plasmids 101: Positive...Joachim Goedhart Guest blogger Joachim Goedhart shares some strategies for generating GFP fusion proteins...proteins. From considering protein size and shape, linker sequences, and fusion location, there are many ...

...as spook, spookier, phantom, disembodied, shadow, and shade. Tongue-eating louse Cymothoa exigua is a ...(yes, Blugene made an appearance there too) and shared fascinatingly creepy science stories. Here are ...

... for their essential advice and service. Joanne shared a moving thank you email from a requestor in Iraq...was once a complicated process of requesting and sharing plasmids has become simplified through Addgene.... our impressive stats from 10 Years of Plasmid Sharing Or read more about what happens Inside Addgene ...

...greatest good you can do for another is not just to share your riches but to reveal to him his own." -Benjamin...fulfilling, productive relationships. I will be sharing what I’ve learned along the way in this ongoing...relationships with advisors and others who helped shape their experience. This led to the topic of “What...

...possible before,” said the Broad Institute's Ophir Shalem. “Whole regions of the genome which were not accessible...depends on your experiment and method of selection. Shalem and Sanjana say they kept a high representation... CRISPR-Cas9 Knockout Screening in Human Cells. Shalem O, Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelson...

...communication In between recurring meetings, you should share interesting results or ask questions to your collaborators... using rapid communication tools such as Slack. Share data and documents While having multiple scientists...may sound like something out of your nightmares, shared ownership of materials produced from your collaboration...

...Rachel Leeson. Here at Addgene, we like to help share useful new resources with the scientific community... can be adjusted based on query, reference, and shared allele search preferences to help you identify ... some surprising, and important, findings being shared. For instance, recently Cellosaurus received a ...

...Addgene fulfill its mission of helping scientists share information and, of course, plasmids. My primary...communication but thought that I would be best poised to share the stories and difficulties behind great discoveries... be therapeutic and I’ve certainly done my fair share of venting, I’m glad I did a PhD and even more glad...

...modeling and structure-guided direct evolution, Nathan Shaner and colleagues from The Scintillon Institute, Allele...mNeonGreen, mTFP1, or mWasabi that you would like to share with the scientific community, please deposit the...PMID: 18325109. PubMed Central PMCID: PMC2292683. Shaner, Nathan C., et al. "A bright monomeric green fluorescent...

...critique and figure out what to improve. You can share it with others and get their input as well. Giving...Candidates who have “started something” which they can share on their resumes have a definite advantage in our...with someone who has interesting experiences to share. The conversation builds a relationship for the ...

...built off of the concept of our AAV Data Hub) to share further validation data with you. “Affinity reagents...allow Addgene to further our mission of scientific sharing,” says Dr. Daniela Bourges, Addgene’s interim Executive...transparent resource where researchers can easily share, edit, and access the affinity reagents they need...

...six of us. We were initially strangers but we all shared a passion for DIY Biology and, on occasion, would...plant cell culture and transgenesis he wants to share his knowledge with the community by teaching a DIY...Interview with Darrell Kotton Learn How Plasmid Sharing Speeds Science Resources at Addgene.org Order...

...roles in fluorescent protein development by openly sharing their resources with the research community. To...scientists attend a conference of their choice to share their fluorescent protein based research. This year...thaliana - Pantoea agglomerans interaction. But the shape and metabolism of leaves can be changed by light...

...for further investigation of this pathogen. The sharing and distribution of cell lines from repositories...scientists are able to make when they have access to shared materials, and then pay it forward by making the...databases and resources Learn about Addgene's work to share plasmids to all scientists Resources on Addgene.org...

...class twice a week at Addgene HQ, but now we’re sharing links to online yoga classes. Get outdoors. Taking...activities like team lunches, a virtual soup lunch, and sharing photos of our pets. #SocialDistancing may send... and CV. Attend virtual workshops and webinars. Share your work online. Maybe you’ve always wanted to ...

... visiting scientist is distinct from equipment sharing or a collaboration – it requires re-location of...the conversation. Many researchers are open to sharing knowledge and establishing collaborations, so it...priority list of experiments you hope to complete. Share this plan with your host prior to arrival so that...

...else to follow them, to being open and willing to share with other researchers to help them troubleshoot... and understand. All of this communication and sharing fits together to make for a transparent and collaborative... for data management plans and for evidence of sharing for grant renewal—but there is clearly room to ...

... a radioactive tracer. As a solution, Mikhail Shapiro’s lab has developed four acoustic reporter gene ...water from entering their hollow interiors. The Shapiro lab discovered in 2014 that these gas vesicle proteins...immune cells has been a gap in the field. Arlene Sharpe’s group at the Harvard Medical School developed ...

... followed soon by GCE done in mammalian cells (Shandell et al., 2021).   How it works If you’re interested...their phosphoserine ncAA protocol, to optimize and share, they’re soliciting feedback from scientists on ...182–188. https://doi.org/10.1126/science.2649980 Shandell, M. A., Tan, Z., & Cornish, V. W. (2021). Genetic...

... navigate when mapping out a new project. We’ve shared some of the concerns that Revolution Bio had to...scientist, and how you were able to overcome them. Share your stories in the comments below! And if you have...developed a genetic tool for plant engineering, please share it with Addgene to both build the plant synthetic...