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Showing: 121 - 140 of 181 results

  1. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...the goal of ‘fixing’ mutations or introducing a desired edit, but this particular class of treatments has...

  3. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...reverse transcriptase (RT) template including the desired edit. Figure 1: Prime Editing schematic....

  4. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ... inhibitor response 1 (TIR1) expressed under a desired promoter with a specific spatiotemporal expression...

  10. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ...This will make it difficult to properly gate your desired cell population. By compensating between fluorescent...

  11. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...advantages over the alternative systems – CRISPR, siRNA, etc. Firstly, they are reversible mechanisms of...

  12. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...Optimized dicot prime editing enables heritable desired edits in tomato and Arabidopsis. Nature Plants,...

  14. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ... recombination can occur in multiple ways. The desired recombination event will remove NeoR only and leave...

  16. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...exchange will prevent your cultures from reaching the desired density. The volume of your flask or culture tube...

  17. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ... be independently validated to ensure that the desired modification produces the phenotype you screened...

  18. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...alternatives Depending on your sequencing result and desired analysis, BLAST may not always be your optimal ...

  19. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ... with secondary nanobodies conjugated with the desired fluorophores. In figure 4B below, triple colocalization...
Showing: 121 - 140 of 181 results