... Cas9 activity in my stable A549 cell pools. Using this virus, it’s expected that Cas9 activity, guided...Cas9 (see tip 2). Pro-Tips 1. To quantify Cas9 activity using this assay, you really need to use FACS ...described in Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9...

...Griesbeck, Heim, & Tsien, 1999), monitoring kinase activity (Ni, Titov, & Zhang, 2006), quantifying antibody...structural changes due to this bulky insertion are inevitable. Most FP fusions for FRET studies are at the ...commercially available as Lumio Green and Lumio Red from Invitrogen (now ThermoFisher Scientific). These reagents...strategies. FRET experimental design lends itself to creativity and innovation, valuable commodities in any NIH...

...these. Addgenies have been organizing Halloween festivities for years – searching our photo archives I found...bragging rights for the next 12 months. Your team’s creativity, cleverness, and crafting skills have surpassed...tribute to the changing seasons – and they also invited Father Time to the party. They won over our judges...

...annual Deck the Lab competition drew upon the creativity of the community, who delivered impressive science-themed...In third place is Matthijs Dorst’s Deck the Lab Nativity scene below his Christmas tree! It is that time...up at home!This year with a #DeckTheLab themed nativity scene, courtesy of our house pets.. 😁 pic.twitter.com...

...developed a platform called SHERLOCK (specific high-sensitivity enzymatic reporter unlocking) to detect single...further to provide multiplexed detection, higher sensitivity, and more. Read the paper in Science Find...dual-nuclease Cas9-Cas9 chimeras with higher target site activity and more predictable and precise deletion products...

... other projects, using stem cells to develop in vitro models for lung disease. Darrell strives to promote...reliable projects. Chapter 9: Using stem cells as in-vitro models of disease. How Darrell is using stem cells...reagents, cell lines, data, and knowledge without exclusivity. Chapter 11: The Stem Cell Bank (CReM Bank) at...

... a colorful makeover Even without its nuclease activity, catalytically dead Cas9 (dCas9) has many applications... Another caveat of the study is the method’s sensitivity. The authors estimate that a minimum of 150-200...necessary for a detectable signal, limiting the sensitivity of the technique. One potential solution is SunTag...

...can we shape our time in academia to maximize productivity and success? We asked leading professors for...suggest that you make time for extracurricular activities both to develop new skills and to discover what...about the benefits of pursuing extracurricular activities. Success evolves from passion True success evolves...

...substrate OR production of a product OR enzyme activity. Small molecule biosensors Addgene has many sensors...constructs are often used to monitor enzymatic activity or phosphorylation over time. You can also use...plate well). Use to track a process (e.g. enzyme activity) over time or under various conditions Localization...

...include fairground rides and other fun activities and even invite members of the public to attend. We thought...aim to support events and scientific community activities throughout Europe and the world. So get in touch...

...several important shortcomings including low processivity (ie. the probability of the polymerase continuing...heterogeneous RNA samples. Furthermore, such high processivity enzymes and the structural understanding thereof...to existing technologies based on polymerase processivity. Please find the MarathonRT plasmid here! Zhao...

...purity or infectivity of the final viral preparation. Through sugar detection assays, in vitro transduction...show that there is no difference in purity or infectivity between the untreated and sucrose treated viral...

...available allowing you to chemically control neuronal activity while studying your phenotype of interest. Optogenetic...Optogenetic tools for similarly controlling neuronal activity with light will be available soon. We’re also ...sharing will only help research advance. Remember: productivity is infectious!  Additional Resources on the...

...resource list available from this post. Use an activity to start a discussion. The Forced Choice Analysis... can use it to clarify and discuss values. The activity is just the start – it is the discussion after...mentoring blog posts, the "Forced Choice Analysis Activity", the "Peer Mentor Groups Guide", "Tips for Choosing...

...allow researchers to independently control the activity of two populations of neurons at once. Addgene...is a toolset that lets you turn the electrical activity of neurons on or off using pulses of light. By...both activators as well as silencers of neural activity. Since then, there has been a steady stream of...

...genome editing tool. However, Cas9’s endonuclease activity can be limited by the need for a specific PAM ...are ion channels that can be used to silence the activity of neurons. Earlier this year, the Hegemann lab...valuable optogenetics feature when studying neuronal activity. The researchers identified MerMAIDS from their...

... Chiasson is currently using VAMP-seq to study vitamin K epoxide reductase (VKOR) variants. VKOR is an...assays for measuring human serotonin transporter activity. Unger also plans to use the serotonin receptor...genotype the patient and rapidly test the variants in vitro against different drugs and dosages.  Michal Bernach...

...transduction profiles in different AAV serotypes both in vitro and in vivo when compared to the conventional AAVs...et al., 2017), exo-AAV2 in mouse retina after intravitreal injection to transduce bipolar cells and photoreceptors...robust gene delivery into the murine retina upon intravitreal injection." Scientific reports 7 (2017): 45329...

... on bacterial expression systems here) and the activity of the plant compounds on these proteins will ... be tested. Investigating changes in neuronal activity after ischemic brain injury Dr. Gohar Tsakanova...image cortical neurons and study changes in neural activity after ischemic brain injury. Additionally, optogenetic...

...we are missing is the unmeasurable skills and productivity coming from that casual discussion on the way...this is an underrated ritual that really drives creativity and novelty in scientific research and in any...springboard for future discussion. Collaboration and creativity are pivotal in writing publications/grants too...