We narrowed to 153 results for: cycs

... tag (Figure 1c). Click chemistry (e.g., transcyclooctene–tetrazine)– Nonnatural amino acids can be ...

... different templates. All PCRs are run as an 18 cycle, 25 μl single-tube reaction. If you are synthesizing...

...dNTPs and then incubate with a standard PCR thermocycling program. One key difference is the plasmid DNA...

...researchers to study aspects of the EBOV replication cycle under BSL-2 conditions (4). What is a minigenome...

...Now, we could iterate our development and testing cycle faster due to the ease of jumping back and forth...

... plasmids for multicopy integration. (B) Anhydrotetracycline (aTc) induces expression transposases leading...

...dCas9-VPR repressor in hPSCs is also driven by a doxycycline inducible promoter. The gRNA piggyBac plasmids...

... in the 80s and 90s, Joe was that kid who used recycled notebook paper, despite the fact that it was gray...

...with Cas9 on an integrative plasmid containing tetracycline repressor TetR and resistance selection marker...

...hydra have asexual and sexual reproductive life cycles. One can inject plasmid DNA in the eggs to get ...

...while aptamers are more stable and can go through cycles of denaturation/renaturation. In vivo assays ...

...is probably “a normal day.” The way I broke this cycle was to just start writing. A word of advice: when...

...vector like pLenti CMV Puro DEST (w118-1) or the doxycycline-inducible pLIX_403. The chosen attR destination...

...Improved Properties of FLP Recombinase Evolved by Cycling Mutagenesis.” Nature biotechnology 16(7): 657–62...

...constant temperature, eliminating the need for thermocyclers. This is followed by in vitro transcription ...

...scientists then revise their hypotheses and the cycle begins again. No part of this process is inherently...

...observed no negative effects on cell viability, cell cycle progression, or apoptosis with Cas9/gRNA expression...

...identification of general heat-shock proteins using IP with CycT1 or PolII-CTD antibodies and unlabeled MS in DLD-...

...mCherry etc.), recombinases (Cre, Flp etc.), and tetracycline drug induction systems (ttA or rtTA) are between...

...to subject your membrane to several freeze/thaw cycles to facilitate cell lysis: alternating between 5...