We narrowed to 151 results for: cycs
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TypeBlog PostPublishedMay 12, 2016, 2:30 p.m....dNTPs and then incubate with a standard PCR thermocycling program. One key difference is the plasmid DNA...
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Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog PostPublishedMarch 17, 2016, 2:30 p.m....researchers to study aspects of the EBOV replication cycle under BSL-2 conditions (4). What is a minigenome... -
Hot Plasmids: Fall 2024
TypeBlog PostPublishedNov. 21, 2024, 1:30 p.m....with Cas9 on an integrative plasmid containing tetracycline repressor TetR and resistance selection marker... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog PostPublishedMay 2, 2019, 12:41 p.m....hydra have asexual and sexual reproductive life cycles. One can inject plasmid DNA in the eggs to get ... -
Hot Plasmids - August 2020
TypeBlog PostPublishedAug. 18, 2020, 1:15 p.m....dCas9-VPR repressor in hPSCs is also driven by a doxycycline inducible promoter. The gRNA piggyBac plasmids... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog PostPublishedOct. 19, 2017, 1:54 p.m....Improved Properties of FLP Recombinase Evolved by Cycling Mutagenesis.” Nature biotechnology 16(7): 657–62... -
Bioinformatics at Addgene
TypeBlog PostPublishedJuly 14, 2022, 1:15 p.m....Now, we could iterate our development and testing cycle faster due to the ease of jumping back and forth... -
CRISPR-based Genome Editing Systems For Editing in E. coli
TypeBlog PostPublishedApril 13, 2021, 1:15 p.m.... plasmids for multicopy integration. (B) Anhydrotetracycline (aTc) induces expression transposases leading... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog PostPublishedFeb. 18, 2020, 2:15 p.m.... in the 80s and 90s, Joe was that kid who used recycled notebook paper, despite the fact that it was gray... -
Fluorescent Proteins 101: Aptamer Fluorophores
TypeBlog PostPublishedApril 11, 2017, 2:30 p.m....while aptamers are more stable and can go through cycles of denaturation/renaturation. In vivo assays ... -
How to Write a Scientific Review Article
TypeBlog PostPublishedFeb. 16, 2017, 3:30 p.m....is probably “a normal day.” The way I broke this cycle was to just start writing. A word of advice: when... -
Plasmids 101: Gateway Cloning
TypeBlog PostPublishedJan. 12, 2017, 3:30 p.m....vector like pLenti CMV Puro DEST (w118-1) or the doxycycline-inducible pLIX_403. The chosen attR destination... -
SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas
TypeBlog PostPublishedMay 5, 2020, 1:15 p.m....constant temperature, eliminating the need for thermocyclers. This is followed by in vitro transcription ... -
March for Science
TypeBlog PostPublishedApril 21, 2017, 2:30 p.m....scientists then revise their hypotheses and the cycle begins again. No part of this process is inherently... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog PostPublishedMay 10, 2016, 2:30 p.m....observed no negative effects on cell viability, cell cycle progression, or apoptosis with Cas9/gRNA expression... -
Hot Plasmids: Spring 2025
TypeBlog PostPublishedJune 3, 2025, 1:15 p.m....identification of general heat-shock proteins using IP with CycT1 or PolII-CTD antibodies and unlabeled MS in DLD-... -
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models
TypeBlog PostPublishedApril 5, 2018, 12:42 p.m....mCherry etc.), recombinases (Cre, Flp etc.), and tetracycline drug induction systems (ttA or rtTA) are between... -
Technique: Probe Phage Genomes for Host Binding Proteins
TypeBlog PostPublishedSept. 29, 2016, 2:30 p.m....to subject your membrane to several freeze/thaw cycles to facilitate cell lysis: alternating between 5... -
Recombinase-based State Machines Enable Order-dependent Logic in vivo
TypeBlog PostPublishedJuly 28, 2016, 2:30 p.m....promoters: the BxbI gene downstream of the anhydrotetracycline (ATc)-inducible promoter (PLtetO), the TP901... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog PostPublishedMarch 6, 2018, 1:49 p.m....is present, the probes will ligate. Subsequent cycling through rounds of melting and annealing allows ...