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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 365 results for: erg

Showing: 121 - 140 of 365 results

  1. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    .... Find plasmids from this paper at Addgene. Gootenberg, Jonathan S., et al. "Nucleic acid detection ...plasmids from this paper at Addgene. Abudayyeh, Gootenberg, et al. “A cytosine deaminase for programmable...

  2. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...during exocytotic budding, as the viral particle emerges. Retrovirus life cycle Retroviruses go against...this is where viral vectors and natural viruses diverge. For the viral vector, its job as a tool is done...

  3. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...varieties must stay current with literature and emerging technologies to remain competitive and relevant...scientists that aims to help stay current on new and emerging work by collaboratively discussing recent publications...

  4. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...Hypothalamic CNTF volume transmission shapes cortical noradrenergic excitability upon acute stress." The EMBO journal...PMC4028491. Van Huijstee AN, Mansvelder HD. Glutamatergic synaptic plasticity in the mesocorticolimbic...

  5. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...find ways to tag and curate reagents from these emerging organisms so scientists can easily find the useful...electric fish are excellent systems to study for convergent evolution because electric organs arose in different...

  6. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...https://doi.org/10.1126/science.1231143  Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung....2014.02.001  Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova...

  7. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...positioned PAM without undesired bases nearby that may undergo unwanted editing. Just like with base editing, ... and antibody-activator fusion proteins. The Synergistic Activation Mediator complex, generally the strongest...

  8. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...pool. In addition, the final viral preparation undergoes PCR with primers targeting the transfer plasmid...https://doi.org/10.1089/hgtb.2013.131 Wang, Y., Bergelson, S., & Feschenko, M. (2018). Determination of ...

  9. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...symptoms of the disease. However, there are some emerging treatments that have shown some positive potential...as these new detection techniques and therapies emerge into mainstream use, they will have a much greater...

  10. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ... work she put into this. 5. Reddit forums are evergreen! With all the great questions that were posted...you’ll see below, it pretty nicely displays her energetic, truthful and helpful style: Joanne also provided...

  12. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ...generate a specific sequence change the cell must undergo homology-directed repair (HDR), a distinct cellular...developed a technology called CORRECT, which, by undergoing two rounds of gene-editing enables scarless introduction...

  13. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...sucrose into a toxic metabolite. Cells that have undergone a double crossover will not contain sacB, allowing...are those where the allelic exchange vector has undergone single crossover and integrated into the genome...

  14. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...genetics, and the nervous system. The fly has also emerged as an important human disease model with potential...and read about new systems being developed for emerging model organisms! References Brenner, Sydney. ...

  16. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...messenger cyclic di-AMP in Gram-positive bacteria (Kellenberger et al., 2013). Other variants of Spinach include... 25337688. PubMed Central PMCID: PMC4244833. Kellenberger, Colleen A., et al. "RNA-based fluorescent biosensors...

  18. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...10.1038/s41587-020-0513-4 Kellner MJ, Koob JG, Gootenberg JS, Abudayyeh OO, Zhang F (2019) SHERLOCK: nucleic...2020.04.20.052159 Zhang, F., Abudayyeh, O. O. & Gootenberg, J. S. A protocol for detection of COVID-19 using...

  20. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...Maesner C, Wu EY, Xiao R, Ran FA, Cong L, Zhang F, Vandenberghe LH, Church GM, Wagers AJ. In vivo gene editing...PMC4335351. 9. Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova...
Showing: 121 - 140 of 365 results