...45), there are also initiatives to create more reliable reagents for future research. I’ve talked to many...many researchers who have complaints about the unreliability of antibodies. IPI is working to address this...repeat previously published results. This over-reliance on the perfect story for a “high impact” publication...

...with, making them the go-to choice for studying mammalian biology. Similar to any model, mice are not without...https://doi.org/10.1016/j.cell.2013.04.025 Zhang X, Liang P, Ding C, Zhang Z, Zhou J, Xie X, Huang R, Sun ...srep32565 Resources on the Addgene Blog CRISPR 101: Mammalian Expression Systems and Delivery Methods Learn ...

...mentioned above and is a popular RNAi trigger in the mammalian research community. Fortunately, many tools for...such as in the model flowering plant Arabidopsis thaliana (Li et al., 2013; Zhang et al., 2018) and the ...posts Resources on Addgene.org Find tools for mammalian RNAi Browse all plasmids for RNAi Check out Addgene's...

...is variable transduction of oligodendrocytes, microglia, astrocytes, neurons, and inhibitory neurons. ...Serotype Oligodendrocytes AAV8 > AAV2 & AAV6 Microglia AAV9 > AAV2 & AAV6 Astrocytes AAV8 > AAV2 & ...Anterograde axonal transport of AAV2-GDNF in rat basal ganglia." Molecular Therapy 19.5 (2011): 922-927. PubMed...

..., the Qi lab employed a gRNA-based design more familiar to those using CRISPR in eukaryotes (Qi et al....transcription of one or more genes in E. coli. and in a mammalian cell line (Qi et al., 2013). Two important features...two tandem copies of the gRNA cassette, one can reliably knockdown expression of multiple target transcripts...

...student researchers Arooba Ahmed, Jiachen Lee, and Jillian Parker who published their work on a protein required... Mark Springel, Arooba Ahmed, Jiachen Lee, and Jillian Parker! Special thanks to our sound engineer/producer...

...which are used to deliver Cas9 and other genes to mammalian cells. For this reason, the top requested plasmid...envelope protein under the control of the powerful mammalian CMV promoter. Envelope proteins are a key component...

...started with tissue culture The cultivation of mammalian cells in the lab, or tissue culture as it is commonly...called, is a critical tool for many scientists. Mammalian cells provide scientists with the means to study...

... Figure 3: Need to introduce plasmids into mammalian cells? Use transfection grade DNA. Transfection...the process of inserting an external plasmid in mammalian cells and it will likely require micrograms of...

...these…. 3. Expect to get 500-700 bases of clean reliable DNA sequence Anything less and you might suspect... Lianna Swanson...

...cloning vector. Lentiviral Plasmids -  If you're familiar with the pLKO plasmids you'll note that those ...expression and pLVTHM, a lentiviral plasmid for mammalian gene silencing. TALENs - Genome engineering methods...

...has been shown to work in plant, Drosophila and mammalian cells, and it may find wide use in biological ...efficiently photoconverted in plant, Drosophila, and mammalian cells. Photoconverted GFP is compatible with other...

...’t even answer your question. Does this sound familiar? Well, when it comes to working with Addgene, ...with our new Help Center! Created using the same reliable Zendesk system that powers our email support, ...

...possible to conduct systematic genetic screens in mammalian cells,” David Sabatini, a senior author of one...). Genome-Wide Recessive Genetic Screening in Mammalian Cells with a Lentiviral CRISPR-Guide RNA Library...

...it’s a probiotic strain that can colonize the mammalian intestinal tract and has been used for 100 years... for noninvasive imaging of microorganisms in mammalian hosts. Nature, 553, 86-90. PubMed PMID: 29300010...

...expression system within the Addgene repository is mammalian, but the largest source of unique gene sequences...frequent transfer of genes is from Proteobacteria to mammalian expression vectors. And more broadly, their data...

... can be used to visualize RNA localization in mammalian cells. These tools consist of cobalamin-binding...your own experiments, mCarmine is available in a mammalian expression vector (pcDNA3) and a bacterial expression...

... available for the calcium sensor jGCaMP7 from Janelia, including three jGCaMP7 plasmids now packaged ...iGluSnFR is a useful tool from Loren Looger’s lab at Janelia for neuroscientists to measure glutamate levels...

...were unable to show TtAgo-mediated cleavage in mammalian cells, likely due to high temperature requirements...cleave plasmid and genomic DNA in a variety of mammalian cell lines, with an efficiency similar to that...

...stem cells, dendritic cells, embryonic fibroblasts, glia cells and neurons, and cell death post-transduction... Introduction of macromolecules into cultured mammalian cells by osmotic lysis of pinocytic vesicles. ...