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Showing: 121 - 140 of 694 results

  1. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ... Plasmids Find Additional Posts on Mol Bio Techniques Find Additional Posts on Plasmid Technologies ...This post was contributed by guest blogger Jon Backstrom, a biochemist in the Vanderbilt Eye Institute...

  2. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ...our CRISPR blog posts Download the CRISPR 101 eBook Read our genome engineering blog posts Resources on...This post was contributed by Kelly Hills, founding bioethicist of Rogue Bioethics. On November 25, the...

  3. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    Published
    Oct. 20, 2020, 1:15 p.m.
    ...on career skills development. Back in March, we posted these 25 great tips for scientists working at home... who they might suggest you speak with. See this post that’s all about informational interviews, for more... the Addgene blog Read our science career blog posts Check out how to use social media as a scientist...done the following types of things: led a grad or postdoc association, started a speaker series or biotech...

  5. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ...commonly prepares NGS samples using both pre- and post-amplification DNA and generates PCR sequencing products...plasmids have dropped out, and comparing pre- and post-amplification sequencing results, you should be ...determine the success of your amplification. I hope this post has helped clarify some of the important considerations...

  6. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...cloning post with a few quirks specific to the PCR cloning process - please check out that post if you ...same as those discussed in our restriction cloning post. However, even more than with standard restriction...

  7. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...This post was contributed by Kurt Thorn of the Nikon Imaging Center at UCSF. A common requirement for...photostability, and so on; see the previous blog post for more discussion of these factors). In addition...proteins, but these are beyond the scope of this post. Are your fluorescent proteins compatible with your...

  8. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...they're useful, so we've updated our cover letter blog post to help you write the best one possible!  Should...hiring managers In preparation for updating this post, I took an informal poll of Addgene hiring managers.... and now... you're ready to go! Good luck! This post was originally written by Maria Soriano-Carot in...Cover Letter Guide How to Write a Cover Letter Blog Post from Hubspot ...in the possibility of joining your team as a postdoctoral researcher. Dear Hiring Manager, I am writing...

  9. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ... downregulated, examine a protein’s stability or post-translational modifications, or study how a target...learn more about Western blotting check out our blog post The Basics of Western Immunoblotting.) For mass ...details of these methods are beyond the scope of this post.  We hope that this blog has provided you with a...Immunofluorescence View all of Addgene's antibody posts  Resources on Addgene.org: Find plasmids ...

  10. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...blog Read all of our Plasmids 101 blog posts Read our blog post on 5 factors to consider before choosing...This post was contributed by Jake Watson and Javier García-Nafría from the MRC Laboratory of Molecular...choosing a cloning method Check out all of our blog posts on plasmid cloning Resources on Addgene.org Read...Laboratory of Molecular Biology. Jake Watson is a postdoctoral researcher in neurophysiology at the MRC Laboratory...

  11. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...Avaliable at Addgene Read Our Blog Post on Protein Tags Read Other Posts on Fluorescent Proteins Browse Our... This post was contributed by guest bloggers Erik L. Snapp and Lindsey M. Costantini. "You underestimate...proteins within these compartments undergo significant post-translational modifications including glycosylation... addition of N-glycans [9]. Both of these posttranslational modifications can disrupt FP folding rendering...from Oregon Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz...subcellular environment. Currently, she is a SPIRE postdoctoral fellow at the University of North Carolina at...

  12. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...Hybrid Plasmids Read our Blog Post About Yeast Vectors Read our Blog Post About Tagging Yeast Genes ...including protein expression and correct folding, post-translational modification, protein degradation,...cells. If the problem is that your proteins are not post-translationally modified, co-expression of the enzyme...

  13. New Viral Vectors - Summer 2024

    Type
    Blog Post
    Published
    July 9, 2024, 1:15 p.m.
    ...ready-to-use viral vectors repository? Quite a bit! In this post, we'll share the 20 additions we've made to our ...viral vector services and resources!   This blog post was written by Rachel Leeson and Brian O'Neill.  ...

  14. Summer Fun at Addgene!

    Type
    Blog Post
    Published
    May 12, 2022, 1:15 p.m.
    ...answering all submitted questions via blog post, which we will be posting in the next few weeks.)  Finally, Addgenies...

  15. #DeckTheLab with Tinsel and Test Tubes, Fa La La La La La La La La

    Type
    Blog Post
    Published
    Dec. 2, 2021, 2:15 p.m.
    ...MattNeuro) December 4, 2020 Here’s how to enter: Post your picture or video for either contest on Twitter...the hashtag #DeckTheLab, and tag @Addgene in the post! In January, Addgenies will vote on the entries...

  16. Addgene’s Blog Gets a New Look!

    Type
    Blog Post
    Published
    Jan. 25, 2021, 5:54 p.m.
    ...articles from your favorite bloggers Like a blog post from a certain author and looking for more from ...can read more about the Addgenie behind the blog post and find more of their work. Articles from guest...

  19. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...This post was originally written in 2014 by Kendall Morgan and updated in 2022 by Lucie Wilson. Lucie...and RNA therapies. [6]. In 2014, we wrote a blog post on CRISPR’s potential for monogenetic diseases, ...complex genetic diseases. So we’re revisiting this post to look at CRISPR’s potential for use in treating...

  20. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...This post was originally written by Tyler Ford in 2018. It was updated by guest blogger Abhi Aggarwal...made it clear that it’s time to update our iGluSnFR post! Here, we look at the origins of the system and ... that have happened since our original 2018 blog post. The origins of iGluSnFR   iGluSnFR was developed...
Showing: 121 - 140 of 694 results