We narrowed to 219 results for: sms

1. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

   Type

   Blog Post

   Published

   Dec. 10, 2019, 1:58 p.m.

   ...were larger than previously reported for other organisms, and the Gottschalk group hypothesizes that this...

2. Components of CRISPR/Cas9

   Type

   Blog Post

   Published

   Feb. 2, 2016, 5 p.m.

   ... cut the target. Cas9 homologs from different organisms or Cas9 mutants developed in a variety of labs...

3. Choosing Your Fluorescent Proteins for Multi-Color Imaging

   Type

   Blog Post

   Published

   Oct. 9, 2014, 3 p.m.

   ...no single fluorescent protein optimal for all organisms and that if you want the brightest signal, you...

4. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

   Type

   Blog Post

   Published

   Nov. 5, 2019, 1:28 p.m.

   ... specificity of Cas9s isolated from different organisms and engineered Cas9 variants that were able to...

5. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

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   Blog Post

   Published

   June 25, 2019, 12:54 p.m.

   ...) is the movement of genetic material between organisms. It plays a key role in bacterial evolution and...

6. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

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   Blog Post

   Published

   Dec. 6, 2018, 1:34 p.m.

   ...makes PL a viable method for proteomics in model organisms in which it was previously not an option, including...

7. Plasmids 101: Inducible Promoters

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   Blog Post

   Published

   Jan. 18, 2018, 2:34 p.m.

   ...transcription machinery differs between cell types or organisms, promoters are similarly variable. Bacterial promoters...

8. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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   Blog Post

   Published

   Aug. 6, 2024, 1:15 p.m.

   ...can direct the production of virus with various tropisms.  Packaging using helper-free packaging cell lines...

9. Hot Plasmids - May 2022

   Type

   Blog Post

   Published

   May 26, 2022, 1:15 p.m.

   ...proteomic mapping in both cell culture and whole organisms (see our 2019 overview). Until recently, the approach...

10. AAV Q&A with Tim Miles

    Type

    Blog Post

    Published

    May 17, 2022, 1:15 p.m.

    ...field has settled on ‘best practices’ for model organisms.    Q11: How consistent is expression is when ...

11. Viral Vectors 101: Viral Vector Elements

    Type

    Blog Post

    Published

    July 25, 2023, 1 p.m.

    ..., typically an expression construct, to cells/organisms. This is often cDNA, shRNA, or something similar...

12. Viral Vectors 101: The AAV Retrograde Serotype

    Type

    Blog Post

    Published

    April 27, 2023, 1:15 p.m.

    ...neurons reside.   Impact for scientists While the mechanisms by which retrograde AAV work are not yet understood...

13. Plasmids 101: Yeast Vectors

    Type

    Blog Post

    Published

    Feb. 25, 2014, 7:11 p.m.

    ...DNA transformation system.Unlike most other microorganisms, yeast have both a stable haploid and diploid...

14. Troubleshooting Your Plasmid Cloning Experiment

    Type

    Blog Post

    Published

    Sept. 24, 2019, 12:55 p.m.

    ...want to amplify sequences from a large genome organisms like mouse or human. There are several online ...

15. Plasmid Tools for Microbiome Studies

    Type

    Blog Post

    Published

    June 27, 2019, 12:25 p.m.

    ...Sequencing the 16S rRNA reveals the identity of the organisms present while sequencing of all transcripts gives...

16. RaPID Detection of RNA-protein Interactions

    Type

    Blog Post

    Published

    Aug. 16, 2018, 12:40 p.m.

    ...alternative option but it’s toxic to tissues and model organisms. Detecting RNA-protein interactions with RaPID...

17. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type

    Blog Post

    Published

    June 15, 2017, 2:30 p.m.

    ...Besides the data, new plasmids and/or cells or organisms that are engineered can be shared to simplify ...

18. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type

    Blog Post

    Published

    April 13, 2017, 2:30 p.m.

    ...conditions. Rosella makes it easier to study the mechanisms behind autophagy by tracking what’s being transported...

19. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type

    Blog Post

    Published

    March 3, 2020, 2:15 p.m.

    ...been used to construct plasmids for use in model organisms such as E. coli and yeast. Because this approach...

20. Summer SciComm: Preprints

    Type

    Blog Post

    Published

    Aug. 16, 2022, 1:15 p.m.

    ...scientists communicate with each other: from formal mechanisms like publishing a research article, presenting...