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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 836 results for: Eras

Showing: 691 - 700 of 836 results

  1. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...creating stable cell lines and are not used for general plasmid selection. Instead, reporter genes are ...material and will subsequently pass it on to future generations. Stably transfected cells may be considered an...

  2. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    Published
    Aug. 20, 2024, 1:15 p.m.
    ...(MFI) of the different populations. MFI is the average brightness from all cells that are positive for...background in protein mutagenesis and cancer immunotherapies. She is currently a Research Assistant in Arlene...

  4. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ... to pursue their scientific curiosity but can literally put their work on the shoulders of giants. The... and blueprints has the potential not only to accelerate scientific progress, but also to enable science...

  5. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ... of an antibody isotype? An isotype determines several key characteristics of an antibody as well as the...in their heavy chain constant regions result in several unique physical characteristics of these five isotypes...

  6. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...CRISPR gene editing methods relied solely on Cas9. Overall, these approaches have two main drawbacks: 1) ...plasmids; for multiple edits, single plasmids are generally more efficient. Pro-Tip Make sure to reverse the...

  7. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...MOI, often resulting in a drop in transduction coverage compared to just one vector.   Q2: Do you think...a desire for very high MOI on top of very high coverage. Please see this paper for more information about...

  8. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...expressing recombineering proteins Red/ET from a temperature-sensitive plasmid. Homologous recombination then.... Using commercially available high-fidelity polymerases such as KOD and Phusion, which are recommended...

  9. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...they examined gRNA expression levels and indel generation for two PTG constructs in rice protoplasts. Compared...human cells. Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK. Mol Cell. 2014 May 22;54(4):698-710. ...

  10. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...auxotrophic selection in E. coli? Additional considerations HIS3 L-hisitidine no S. cerevisiae  no yes...ade6+ purine (adenine)   S. pombe  no no   Considerations when using auxotrophic selection Of course,...
Showing: 691 - 700 of 836 results