We narrowed to 842 results for: Eras

... to pursue their scientific curiosity but can literally put their work on the shoulders of giants. The... and blueprints has the potential not only to accelerate scientific progress, but also to enable science...

... of an antibody isotype? An isotype determines several key characteristics of an antibody as well as the...in their heavy chain constant regions result in several unique physical characteristics of these five isotypes...

...MOI, often resulting in a drop in transduction coverage compared to just one vector.   Q2: Do you think...a desire for very high MOI on top of very high coverage. Please see this paper for more information about...

...CRISPR gene editing methods relied solely on Cas9. Overall, these approaches have two main drawbacks: 1) ...plasmids; for multiple edits, single plasmids are generally more efficient. Pro-Tip Make sure to reverse the...

...expressing recombineering proteins Red/ET from a temperature-sensitive plasmid. Homologous recombination then.... Using commercially available high-fidelity polymerases such as KOD and Phusion, which are recommended...

...they examined gRNA expression levels and indel generation for two PTG constructs in rice protoplasts. Compared...human cells. Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK. Mol Cell. 2014 May 22;54(4):698-710. ...

...auxotrophic selection in E. coli? Additional considerations HIS3 L-hisitidine no S. cerevisiae  no yes...ade6+ purine (adenine)   S. pombe  no no   Considerations when using auxotrophic selection Of course,...

...  Prime editing improvements By Angela Holmes Several labs have recently shared some exciting plasmid...systems improve gene editing performance at therapeutically relevant gene targets and expand the application...

...allowing an influx of ions into the neuron which generates an action potential (Figure 1). This light-induced..., must be optimized simultaneously in order to generate a channelrhodopsin that’s successful in vivo. ...

...human embryos has been met with a storm of media coverage. But the paper published August 2nd in Nature ...present.   Another issue surrounding CRISPR-based therapy is the level of testing needed to define whether...