We narrowed to 842 results for: Eras

...individual colors to 6. A gRNA with all three hairpins generates white light. All 6 gRNAs can be expressed in a...separate vector. Figure 2: CRISPRainbow generates 6 unique colors and a white composite. gRNAs with...

...plasmids, such as special growth instructions (temperature, strain, media supplements), suggested sequencing...re like us, you need a strategy for processing several sequencing reactions in parallel. We have a few...

...GBPb-GAL4 (DNA-binding domain); and a UAS-driven luciferase reporter construct. In the absence of GFP, no...create a complete transcription factor, activating luciferase transcription. Tang et al. coined this system...

...and synthetic biology Synthetic biologists have leveraged the power of Golden Gate cloning into a modular...cloning or Gibson assembly. Another important consideration is the design of flanking overhangs. Although...

...through programming cells. In the context of therapeutics, if you want to program a cell to identify and...Sometimes in CRISPR people talk about off-target interactions. That’s relevant if you want to make sure you...

...binding biotin), is conjugated to the enzyme horseradish peroxidase (HRP). Finally, the substrate of HRP... mx+b?). Figure 5: A standard curve is generated by plotting OD against known concentrations of...

...monoclonal hybridomas, but those techniques have several drawbacks. Polyclonal antibodies produced in animals...antibodies are monoclonal antibodies that are generated in vitro using synthetic genes that are typically...

... of society or devoid of social context. This “general public” audience comprises the taxpayers who fund...be affected by the knowledge and applications generated from this research. At both a practical and ethical...

...recognized that genome sequencing projects were generating huge amounts of data that could provide the basis... to genes in other species, but these studies generally leave questions about gene function wide open....

...folks at the makerspace comfortable with next-generation sequencing data and the bioinformatics pipeline...resources to pursue it. Since makerspaces are generally funded on a membership model - everyone contributes...