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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 836 results for: Eras

Showing: 721 - 730 of 836 results

  1. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...process has changed with the introduction of next generation sequencing!  Choosing a BLAST program Of the...amount of time to perform the search as well as the overall alignment results. The default setting is “Optimize...

  2. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...7 mutations found in the REC2, REC3, and PAM interacting domains and allows for expanded PAM recognition...engineering was Cas12a (formerly Cpf1), a nuclease that generates double strand breaks in target genes resulting...

  3. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...remotely-tethered ligands (PORTLs). OptoXRs are chimeras of the extracellular domain of endogenous light-sensitive...Created with BioRender.com.   Important considerations for using optogenetic tools Once you’ve selected...

  4. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    .... During FACS, the sample stream oscillates to generate droplets that are charged as they pass through... cells. Once in the cells, amine reactive dyes interact with free amines in the cytoplasm causing dead...

  7. The 12 Days of CRISPR

    Type
    Blog Post
    Published
    Jan. 3, 2023, 2:15 p.m.
    ...variants for targeted RNA degradation with minimal collateral effects🎵 On the 1st day of CRISPR, Addgene gave...

  9. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    Published
    May 13, 2019, 12:01 p.m.
    ...CRISPR overview and history. Tuesday will focus on generating knockouts using CRISPR and Wednesday will highlight...

  10. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    Published
    Feb. 4, 2015, 3:58 p.m.
    ...adoption of the Cas9 technology was also greatly accelerated through a combination of open-source distributors...
Showing: 721 - 730 of 836 results