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We narrowed to 729 results for: NSI

Showing: 71 - 80 of 729 results

  1. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...first-generation APEX suffered from inconsistent activity levels and limited sensitivity under the stringently low...extremely dim GFPs The availability of the three dimensional structure of GFP, its brighter mutant S65T-GFP...GFPs with diversity in fluorescence brightness, intensity, excitation and emission spectra. The lab of Dimitri... degradation. Chemical approaches may require expensive peptides or small molecules and exceed a physiologically...physiologically relevant exposure in intensity and/or duration. Both of these strategies carry the risk of...activate signaling cascades on exposure to low-intensity blue light. This approach relies on the incorporation...incorporation of light-oxygen-voltage (LOV) sensing domains from algae into chimeric Opto-RTKs. The LOV domains...

  2. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ...brain. Sensitivity In ex vivo studies on mouse brain slices, nLightG and GRABNE were more sensitive than...But this first generation of tools had limited sensitivity and speed and were only available as green probes... vivo experiments. nLight indicators are more sensitive to and selective for norepinephrine and faster...that red fluorescent proteins tend to be less sensitive than green fluorescent proteins. nLightR did perform...and may soon have a red tool which matches the sensitivity of nLightG. Many thanks to Mike Waring and Tommaso...Bohacek, J., Reiner, A., … Patriarchi, T. (2023). Sensitive multicolor indicators for monitoring norepinephrine...

  3. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...antibodies when possible. If cost is prohibitive, consider an approach that prioritizes more reproducible...with representative images showing the staining intensity in the KO population (pink histogram) compared...knockout experiments can be time-consuming and expensive, it may be helpful to find an antibody that has...RNA (siRNA). Knockdown caused by siRNA is also transient, and as such, the optimal knockdown of the protein... rate. If you need a more permanent knockdown, consider using shRNA vectors instead. With both siRNA and...understand the relationship between antibody staining intensity by flow cytometry, and mRNA expression.   ... at similar levels in your cell lines, you can consider a cell treatment approach. If there is a cell ...

  4. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    Published
    April 9, 2019, 1:13 p.m.
    ... This is an important downside of the technology and should always be considered when interpreting results...oligomerization of fluorescent proteins is a concern. The propensity of fluorescent proteins to oligomerize is known...brightness, photostability, maturation time, and acid sensitivity of various fluorescent proteins (Botman et al...coded by the multiple cloning site and therefore consist of random amino acid sequences or (ii) designed... an inert, unstructured peptide and therefore consisted of glycines, serines and other small, non-aliphatic...from Aequorea victoria GFP sticks out and can be considered a linker (figure 1). In fact, for several FRET...fluorescent protein fusions There are several choices to consider before generating a fusion protein, e.g. linker...

  5. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ... other ligands - almost A to Z! - from ATP to urotensin. The MTRIA sensors will be useful for investigating...displays enhanced brightness, expression, and sensitivity compared with earlier SF-iGluSnFR. Image from... response kinetics and increased brightness, sensitivity, and photostability under two-photon illumination...proteins (miRFP670nano and miRFP720) and the Ca2+-sensing domain from Twitch-2B (Matlashov et al. 2022). ...plasmids here!   Torres Cabán CC, et al. Tuning the Sensitivity of Genetically Encoded Fluorescent Potassium ...LiLac has improved response size and reduced sensitivity to pH and calcium compared to previous lactate...470-495 nm) and is capable of local temperature sensing with sub-millisecond resolution and sub-degree ...

  6. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ... Morgridge Institute for Research in Madison, Wisconsin. It is commonly cited that approximately one-third...modified through phosphorylation (1). However, the expansion of studies on protein phosphorylation in an array...The development of phosphoserine incorporation Extensive genomic sequencing of prokaryotic lineages revealed...specialized elongation factor that allows proper extension and translation of the pSer-incorporated polypeptide...available through Addgene, but we have worked most extensively worked with and optimized Sep-OTSλ and can recommend...the deletion of release factor 1, the protein responsible for terminating translation in response to the...between different proteins. Beyond these standard considerations, it is important to determine how much phosphorserine...

  7. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...integrating a SL-insensitive fluorophore (LSSmOrange) as an internal control and a SL-sensitive fluorophore...organoids By Ashley Waldron Organoids are three-dimensional cultures that mimic aspects of organ morphology...enzyme biosensors provide direct, specific and sensitive detection of the plant hormones strigolactones...the receptor which modulates the fluorescence intensity of the cpGFP without altering that of the internal...fluorophores, rather than on the fluorescence intensity, it provides a direct and quantitative detection...receptor-based fluorescent biosensor has a high sensitivity (50 nM-500nM range) and a high specificity for...

  8. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...temperature drops, single intensity should smoothly increase as the DNA amplicon transitions from single-stranded...detect a single copy of a transcript. But this sensitivity can sometimes cause as many issues as it solves...separate thermocycle allows you to measure the intensity of signal over an entire cycle. Since signals ...double-stranded DNA, when temperatures are high, signal intensity should be low, reflecting the presence of primarily...assumption that there is a smooth, single-step transition between ssDNA and dsDNA. This is not true for...one (Biassoni, 2014). At the bench As a highly sensitive technique, qPCR needs a steady pair of hands to...important as precision for relative quantification. A consistent 5% positive error across the plate is fine; a...

  9. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...should be consistent across all samples on the gel and should be observed as a band of consistent size and...These systems, however, require you to purchase expensive system-specific sandwiches.   Figure 2:...vendors will show that the antibody binds to transiently overexpressed protein, however, they should also...includes publications that cited the antibody, consider reviewing the materials and methods sections of...and density present in all samples. Uneven sample loading or incomplete protein transfer causes inconsistent...you require precise quantification of a protein, consider using an alternative method such as an enzyme-...

  10. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...ultimately responsible for outcomes. Be ready to support your team and take responsibility if things go...series on Management for scientists. Once you are responsible for managing others you will only be successful...you become a master at delegating tasks and responsibilities. The manager is not expected to DO all the...experience? Do you think you are giving this responsibility to someone who can do the job. It is good to...sure it goes well? As with most managerial responsibilities, good communication is at the heart of delegating...boundaries. Where are the lines of authority, responsibility and accountability? What types of issues do...
Showing: 71 - 80 of 729 results