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Showing: 211 - 240 of 285 results

  3. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...proximity with the target protein, leading to polyubiquitination of the target protein and proteasomal degradation...

  7. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...vector. Full-length ssAAV vector genome of both polarities are rescued from the vector plasmid and individually...

  8. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...to detect variants such as single nucleotide polymorphisms. Fast facts – PAM requirement: none. Best ...

  9. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...comment online! Want the general public (say, policymakers or community organizations) to use your work...

  16. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...is caused by a variety of single nucleotide polymorphisms (SNPs) in the TTR gene and is molecularly characterized...

  17. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ... and biological. Even the most high-fidelity polymerases can make mistakes and lead to the accumulation...

  18. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ... La to protect the end of the pegRNA. Adding 3′ polyU tracts to the end of pegRNAs (but not epegRNAs) ...

  23. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...the surface of the protein where the nascent polypeptide emerges from the exit channel.   He then said...

  28. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...rAAV from your supernatant, you can simply use polyethylene glycol (PEG). We add 25 mL of 40% PEG solution...

  29. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...endogenous CRBN E3 ligase complex which will polyubiquitinate the fusion protein. This will target the protein...

  30. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...transporting proteins in yeast by using murine polyomavirus virus-like particles (MPyV VLPs ). This artificial...
Showing: 211 - 240 of 285 results