We narrowed to 291 results for: Pol

...III. Gene III is under the control of a T7 RNA polymerase and cannot be expressed until the base editor...

...broader impacts for their work in education and policy and to draw attention and support to work in their...

...Promoters control the binding of RNA polymerase and transcription factors. Since the promoter region ...

...previously mentioned, enlist the help of your network to polish the final draft prior to submission. When in doubt...

...whose work is focused on education and education policy. Additional Resources on the Addgene Blog Synthetic...

.... Using commercially available high-fidelity polymerases such as KOD and Phusion, which are recommended...

...vector. Full-length ssAAV vector genome of both polarities are rescued from the vector plasmid and individually...

...spectroscopic methods (based on fluorescence polarization) can be used to detect homodimerization. However...

...comment online! Want the general public (say, policymakers or community organizations) to use your work...

...proximity with the target protein, leading to polyubiquitination of the target protein and proteasomal degradation...

...companies selling PCR reagents. Nowadays commercial polymerases are very efficient, but if you are having difficulties...

... have a higher conductance, so they can still depolarize neurons at lower MOIs. Figure 2: ChRgers...

...to detect variants such as single nucleotide polymorphisms. Fast facts – PAM requirement: none. Best ...

...used to create any program you can imagine. The Apollo 11 mission circuitry that took a spaceship to the...

...PhDs and postdocs posted by Toronto-based Jennifer Polk of From PhD to Life. She runs great tweet chats,...

... and biological. Even the most high-fidelity polymerases can make mistakes and lead to the accumulation...

... La to protect the end of the pegRNA. Adding 3′ polyU tracts to the end of pegRNAs (but not epegRNAs) ...

...antibodies. You’ll want to set up your blot with a polyvinylidene difluoride (PVDF) membrane, which is sturdier...

...is caused by a variety of single nucleotide polymorphisms (SNPs) in the TTR gene and is molecularly characterized...

... P, Kocak DD, Vockley CM, Adler AF, Kabadi AM, Polstein LR, Thakore PI, Glass KA, Ousterout DG, Leong ...

...expression is activated through the  induction of T7 polymerase encoded in the E. coli genome (usually controlled...

... C. elegans 3’UTRs structures, alternative polyadenylation for all protein coding mRNAs, and annotations...

... CRISPR human genome-wide library deposited by Leopold Parts. This library delivers 2 randomly paired ...

...technology park, it enjoys preferential governmental policies for a high-tech development zone so many science...

...-aggressives, who are usually antagonistic and impolite, Complainers, who spend most of their time moaning...

...References and resources References Duret, G., Polali, S., Anderson, E. D., Bell, A. M., Tzouanas, C....

...rAAV from your supernatant, you can simply use polyethylene glycol (PEG). We add 25 mL of 40% PEG solution...

...endogenous CRBN E3 ligase complex which will polyubiquitinate the fusion protein. This will target the protein...

..., Cas9 forms a steric block that prevents RNA polymerase from efficiently transcribing the gene. This ...

...transporting proteins in yeast by using murine polyomavirus virus-like particles (MPyV VLPs ). This artificial...