We narrowed to 291 results for: Pol

...2016 Jun 30;60(1):1-8. DOI: 10.1042/EBC20150001 Leopold, A. V., Shcherbakova D. M., Verkhusha, V. V., Fluorescent...

...whose work is focused on education and education policy. Additional Resources on the Addgene Blog Synthetic...

...exciting computational algorithms, such as SIFT or PolyPhen to approach this challenge, using attributes of...

...PMCID: PMC3773023. 7. Vouillot, L., Thélie, A. & Pollet, N. Comparison of T7E1 and surveyor mismatch cleavage...

...previously mentioned, enlist the help of your network to polish the final draft prior to submission. When in doubt...

.... Using commercially available high-fidelity polymerases such as KOD and Phusion, which are recommended...

...proximity with the target protein, leading to polyubiquitination of the target protein and proteasomal degradation...

...comment online! Want the general public (say, policymakers or community organizations) to use your work...

... have a higher conductance, so they can still depolarize neurons at lower MOIs. Figure 2: ChRgers...

...companies selling PCR reagents. Nowadays commercial polymerases are very efficient, but if you are having difficulties...

...vector. Full-length ssAAV vector genome of both polarities are rescued from the vector plasmid and individually...

...spectroscopic methods (based on fluorescence polarization) can be used to detect homodimerization. However...

...to detect variants such as single nucleotide polymorphisms. Fast facts – PAM requirement: none. Best ...

...used to create any program you can imagine. The Apollo 11 mission circuitry that took a spaceship to the...

...PhDs and postdocs posted by Toronto-based Jennifer Polk of From PhD to Life. She runs great tweet chats,...

...expression is activated through the  induction of T7 polymerase encoded in the E. coli genome (usually controlled...

... CRISPR human genome-wide library deposited by Leopold Parts. This library delivers 2 randomly paired ...

... P, Kocak DD, Vockley CM, Adler AF, Kabadi AM, Polstein LR, Thakore PI, Glass KA, Ousterout DG, Leong ...

... C. elegans 3’UTRs structures, alternative polyadenylation for all protein coding mRNAs, and annotations...

...is caused by a variety of single nucleotide polymorphisms (SNPs) in the TTR gene and is molecularly characterized...

...antibodies. You’ll want to set up your blot with a polyvinylidene difluoride (PVDF) membrane, which is sturdier...

... and biological. Even the most high-fidelity polymerases can make mistakes and lead to the accumulation...

... La to protect the end of the pegRNA. Adding 3′ polyU tracts to the end of pegRNAs (but not epegRNAs) ...

...technology park, it enjoys preferential governmental policies for a high-tech development zone so many science...

...-aggressives, who are usually antagonistic and impolite, Complainers, who spend most of their time moaning...

... (cap) genes. AAV replication uses the host’s polymerase, but requires Rep proteins to process a double-stranded...

...catalytically dead SpCas9 (dCas9) fused to the bacterial polymerase subunit ω. This construct is designed to activate...

...transporting proteins in yeast by using murine polyomavirus virus-like particles (MPyV VLPs ). This artificial...

...the surface of the protein where the nascent polypeptide emerges from the exit channel.   He then said...

...HaloTag, SpyTag). The Foerstemann lab developed a polymerase chain reaction (PCR)-based system that allows...